CAJ | 학술논문

目的对2009年武汉市新分离的2株乙型脑炎（简称乙脑）病毒进行基因分型和序列分析，了解本地乙脑病毒株的分子生物学特性。方法将2009年从三带喙库蚊中分离的两株乙型脑炎病毒用RT-PCR法扩增E基因，将其进行测序，并用DNAstar and MegAlign软件与其他基因型代表株进行比对。结果 16组样品检出两株阳性（WHJX9-09、WHJX10-09），这两株阳性均属于GI型。两株新分离JEV之间的核苷酸和氨基酸同源性分别为98.9％和100％。同目前在武汉市使用的疫苗株SA-14-14-2相比，核苷酸同源性分别为87.4％、87.9％，氨基酸同源性为96.9％。共有15个氨基酸发生变异分布在3个不同结构域，中和位点没有变异但是神经毒力位点仍然存在。结论武汉市本地新分离乙脑病毒基因型为GI型，不同于1988年在武汉检出的GⅢ型的基因型，和疫苗株SA-14-14-2相比，其神经毒力并没有减弱，但疫苗产生的抗体对新出现的GI型乙脑病毒仍有中和作用。因此提高乙脑疫苗的接种率并配合防蚊灭蚊措施对控制乙脑疫情依然至关重要。同时有必要对本市蚊虫及乙脑患者进行长期的病原学监测工作，为乙脑预测预警体系的建立提供科学依据。
목적 대2009년무한시신분리적2주을형뇌염（간칭을뇌）병독진행기인분형화서렬분석，료해본지을뇌병독주적분자생물학특성。방법 장2009년종삼대훼고문중분리적량주을형뇌염병독용RT-PCR법확증E기인，장기진행측서，병용DNAstar and MegAlign연건여기타기인형대표주진행비대。결과 16조양품검출량주양성（WHJX9-09、WHJX10-09），저량주양성균속우GI형。량주신분리JEV지간적핵감산화안기산동원성분별위98.9％화100％。동목전재무한시사용적역묘주SA-14-14-2상비，핵감산동원성분별위87.4％、87.9％，안기산동원성위96.9％。공유15개안기산발생변이분포재3개불동결구역，중화위점몰유변이단시신경독력위점잉연존재。결론 무한시본지신분리을뇌병독기인형위GI형，불동우1988년재무한검출적GⅢ형적기인형，화역묘주SA-14-14-2상비，기신경독력병몰유감약，단역묘산생적항체대신출현적GI형을뇌병독잉유중화작용。인차제고을뇌역묘적접충솔병배합방문멸문조시대공제을뇌역정의연지관중요。동시유필요대본시문충급을뇌환자진행장기적병원학감측공작，위을뇌예측예경체계적건립제공과학의거。
Objective To analyze the molecular characteristics of the newly isolated two Japanese encephalitis virus strains(JEV) in Wuhan. Methods The mosquitoes were collected in Wuhan from April to October in 2009. The envelope (E) protein gene of JEV was detected using RT-PCR and sequenced.Sequence comparisons and phylogenetic analysis were conducted using DNAstar and MegAlign. Results Two Japanese encephalitis virus (JEV) strains (WHJX09-9, WHJX09-10 ) were isolated from Culex tritaeniorhynchus among 16 mosquito pools and identified as genotype I. The result showed that the homology of the two strains was 98. 9％ in nucleotides and 100％ in deduced amines. The comparison between the new genotype 1 JEV strains and live attenuated vaccine strain SA14-14-2 in E gene showed that the homology of nucleotide sequence was 87.4％ and 87.9％ ,the homology of amino acid was 96. 9％ (totol 15 amino acid were different) in E gene. The mutation sites of amino acid distributed among three different coding domain,but no antigen binding site and neurotoxin-involved site of amino acid were changed. Conclusion Wuhan had appeared a new genotype of JEV which was different from the former strain isolated in Wuhan,the new JEV strains still had neurotoxicity but had high homology with the vaccine strains adopted in Wuhan. The vaccine could still be adopted to prevent Japanese encephalitis if steps were take to eradicate mosquitos at the same time. laboratory surveillance were also an important task to build an early-warning mechanism against JEV.