CAJ | 학술논문

目的探讨季节性流感疫苗在小鼠中的免疫原性及其使50%小鼠血凝抑制抗体滴度(HI)达到40的血凝素(HA)有效剂量(ED50a).方法以我国2008-2009年度季节性流感裂解疫苗为模式,将该疫苗中针对甲型流感病毒H1N1与H3N2两种组分按照HA不同剂量免疫小鼠,通过比较1针免疫与2针免疫所产生的HI抗体强度探讨季节性流感疫苗在小鼠中的免疫原性,确定疫苗免疫程序;此后,观察两种疫苗组分免疫小鼠后的HI抗体动力学,确定HI抗体产生的高峰期;最后,使用HA不同剂量免疫小鼠,在HI抗体高峰期测定使50%小鼠HI抗体滴度达到40的HA有效剂量(ED50a).结果季节性流感疫苗1针与2针免疫结果显示,两种疫苗组分1针免疫可在小鼠中产生HI抗体滴度范围为10～120,2针免疫可以使HI抗体滴度为1针免疫的10～100倍;HI抗体动力学研究表明,两种疫苗组分1针免疫后第28～35天为HI抗体产生的高峰期;该高峰期的ED50a测定结果表明,两种疫苗组分使50%小鼠HI抗体滴度达到40的HA有效剂量(ED50a)均为1.5μg.结论季节性流感疫苗在小鼠中具有良好的免疫原性,1针免疫后第28～35天为抗体产生的高峰期,使50%小鼠HI达到40的HA有效剂量为1.5μg,为建立以季节性流感疫苗为参考的免疫保护评价体系奠定了基础.
목적 탐토계절성류감역묘재소서중적면역원성급기사50%소서혈응억제항체적도(HI)체도40적혈응소(HA)유효제량(ED50a).방법 이아국2008-2009년도계절성류감렬해역묘위모식,장해역묘중침대갑형류감병독H1N1여H3N2량충조분안조HA불동제량면역소서,통과비교1침면역여2침면역소산생적HI항체강도탐토계절성류감역묘재소서중적면역원성,학정역묘면역정서;차후,관찰량충역묘조분면역소서후적HI항체동역학,학정HI항체산생적고봉기;최후,사용HA불동제량면역소서,재HI항체고봉기측정사50%소서HI항체적도체도40적HA유효제량(ED50a).결과 계절성류감역묘1침여2침면역결과현시,량충역묘조분1침면역가재소서중산생HI항체적도범위위10～120,2침면역가이사HI항체적도위1침면역적10～100배;HI항체동역학연구표명,량충역묘조분1침면역후제28～35천위HI항체산생적고봉기;해고봉기적ED50a측정결과표명,량충역묘조분사50%소서HI항체적도체도40적HA유효제량(ED50a)균위1.5μg.결론 계절성류감역묘재소서중구유량호적면역원성,1침면역후제28～35천위항체산생적고봉기,사50%소서HI체도40적HA유효제량위1.5μg,위건립이계절성류감역묘위삼고적면역보호평개체계전정료기출.
Objective To evaluate the seasonal influenza spilt vaccine's immunogenicity and the 50% effective dose ( ED50a ) of hemagglutin ( HA ) that can make 50% of the mice hemagglutination inhibition antibody ( HI ) titers to 40. Methods The 2008-2009 seasonal influenza spilt vaccine's two components, with HA from H1N1 and H3N2 influenza virus respectively, were used as a model. Mice were immunized once or twice with different doses, and the HI antibody titers were tested to determine the immunization procedure and to evaluate the immugenicity of seasonal influenza spilt vaccine in mice;Consequently, HI antibody response kinetics of the two components were observed to determine the time point when the HI antibody titer reached the peak point; Finally, mice were immunized with different doses of HA to evaluate the ED50a that can make 50% of mice HI titers reach 40. Results Immunization procedures study showed that one-dose of seasonal influenza vaccine induced the HI antibody titem ranged from 10 to 120, while two-dose of influenza vaccine improved the HI antibody titer 10-100 times as compared with one dose; antibody kinetics study suggested that the time point of HI antibody produced to peak is 28-35 days post one dose immunization ; and the ED50a detection results indicated that one dose of 1.5 μg HA could make 50% of the mice HI antibody titer reach 40. Conclusion Seasonal influenza spilt vaccine is very immunogenic in mouse; the time point of HI antibody produced to peak is 28-35 days post one dose immunization; and the ED50a of HA is 1.5 μg, which can make 50% of the mice HI titer reach 40. The experimental results provided foundation for the establishment of influenza vaccine evaluation system based on seasonal influenza vaccine.