CAJ | 학술논문

目的观察人类快速延迟整流性钾通道基因表达的钾离子通道蛋白( HERG1)和血管内皮生长因子(VEGF)在胃癌组织中的表达,探讨两者与胃癌预后的关系.方法应用免疫组织化学方法(SP法)、逆转录－聚合酶链反应(RT-PCR)技术方法对80例胃癌组织标本及对应正常胃黏膜组织中的HERG1蛋白和VEGF的表达进行检测,并进行回顾性随访.结果免疫组织化学、RT-PCR检测胃癌组织中HERG1的阳性表达率分别为60％、90％,VEGF的阳性表达率分别为55％、85％；HERG1蛋白表达与淋巴结转移,远处转移关系密切；VEGF的表达与胃癌的浸润程度、TNM分期、分化程度、淋巴结转移明显相关(P＜0.05),而与性别、肿瘤大小、部位无明显相关.结论 HERG1蛋白和VEGF的过度表达与胃癌的发生发展有关,在肿瘤侵袭、转移过程中起重要作用,其共位表达有助于判断胃癌预后.
목적 관찰인류쾌속연지정류성갑통도기인표체적갑리자통도단백( HERG1)화혈관내피생장인자(VEGF)재위암조직중적표체,탐토량자여위암예후적관계.방법 응용면역조직화학방법(SP법)、역전록－취합매련반응(RT-PCR)기술방법대80례위암조직표본급대응정상위점막조직중적HERG1단백화VEGF적표체진행검측,병진행회고성수방.결과 면역조직화학、RT-PCR검측위암조직중HERG1적양성표체솔분별위60％、90％,VEGF적양성표체솔분별위55％、85％；HERG1단백표체여림파결전이,원처전이관계밀절；VEGF적표체여위암적침윤정도、TNM분기、분화정도、림파결전이명현상관(P＜0.05),이여성별、종류대소、부위무명현상관.결론 HERG1단백화VEGF적과도표체여위암적발생발전유관,재종류침습、전이과정중기중요작용,기공위표체유조우판단위암예후.
Objective To investigate the expression of HERG1 protein and vascular endothelial growth factor (VEGF) in gastric carcinomas and the correlation between HERG K+ channels and VEGF.Methods Reverse transcription and PCR (RT-PCR) and immunohistochemistry (IHC) were used to detect the expression of HERG1 gene and VEGF in 80 gastric carcinomas and the patients were followed-up.Results The positive expression of HERG1 and VEGF was detected in 48 cases (60％) and 44 cases (55％) by immunohistochemistry,and in 72 cases (90％) and 70 cases (85％) by RT-PCR,respectively.There was a significant correlation between the positive expression of VEGF and depth of invasion,TNM stage,differention,lymphatic and distant matastasis in gastric carcinomas ( P ＜ 0.05 ).The positive expression of HERG1 was correlated with lymphatic and distant metastasis.Conclusion HERG K + channels and VEGF overexpression in gastric carcinomas appeared to be correlated with tumor genesis and development,and may play an important role in the tumor invasion and metastasis.The co-expression of HERG K + channels and VEGF could be well-characterized indicator to prognosis of gastric cancer.