CAJ | 학술논문

目的观察E2F1对H2O2诱导的人乳腺癌细胞MDA-MB435凋亡的影响.方法通过脂质体法转染目的质粒pcDNA3.1(+)/E2F1进人人乳腺癌细胞株MDA-MB435,筛选稳定表达E2F1的细胞克隆,通过Western blot法鉴定高表达E2F-1的MB435细胞克隆C1、C2和C3,并应用锥虫蓝染色法检测100 μmol/L H2O2处理后细胞的存活率变化.结果在细胞克隆MB435/E2F1-C1、C2和C3中有高水平的E2F1表达,这些高表达E2F1的肿瘤细胞经100 μmol/L H2O2刺激后细胞存活率(分别为63.4％、54.2％和32.9％)相对于对照组(90.9％)均明显降低,且其降低的水平与E2F1的表达水平成正比,差异有统计学意义(P＜0.01).结论 E2F1对H2O2诱导的人乳腺癌细胞凋亡有促进作用.
목적 관찰E2F1대H2O2유도적인유선암세포MDA-MB435조망적영향.방법 통과지질체법전염목적질립pcDNA3.1(+)/E2F1진인인유선암세포주MDA-MB435,사선은정표체E2F1적세포극륭,통과Western blot법감정고표체E2F-1적MB435세포극륭C1、C2화C3,병응용추충람염색법검측100 μmol/L H2O2처리후세포적존활솔변화.결과 재세포극륭MB435/E2F1-C1、C2화C3중유고수평적E2F1표체,저사고표체E2F1적종류세포경100 μmol/L H2O2자격후세포존활솔(분별위63.4％、54.2％화32.9％)상대우대조조(90.9％)균명현강저,차기강저적수평여E2F1적표체수평성정비,차이유통계학의의(P＜0.01).결론 E2F1대H2O2유도적인유선암세포조망유촉진작용.
Objective To study the influence of E2F1 on signaling oxidative stress-induced apoptosis of MDA-MB435 cells.Methods pcDNA3.1 ( + )/E2F1 was transfected into MB435 cells by using LipofectAMINE and stable cell clones expressing high level E2F1 (MB435/E2F1-C1,C2,C3) were selected,and the expression level of E2F1 was detected by using Western blotting.E2Fl-mediated apoptosis of MDA-MB435 cells treated with 100 μmol/L H2O2 was examined by Trypan blue exclusion.Results There were high levels of E2F1 protein in the three stable clones expressing ectopic E2F1,and the viability of cells with high levels of E2F1 was greatly reduced after treatment with 100 μmol/L H2O2 as compared with the control cells (63.4％,54.2％ and 32.9％ vs.90.9％,P＜0.01).Conclusion E2F1 can increase apoptosis of breast cancer cells in response to H2O2 treatment.