中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2012年
8期
528-531
,共4页
沈黎蔚%王介非%董晓君%宫钰%高婷%周婷婷%李淑婷%杨淑殷%李海
瀋黎蔚%王介非%董曉君%宮鈺%高婷%週婷婷%李淑婷%楊淑慇%李海
침려위%왕개비%동효군%궁옥%고정%주정정%리숙정%양숙은%리해
肝功能衰竭%乙型肝炎%单核细胞%细胞凋亡%细胞增殖%流式细胞术
肝功能衰竭%乙型肝炎%單覈細胞%細胞凋亡%細胞增殖%流式細胞術
간공능쇠갈%을형간염%단핵세포%세포조망%세포증식%류식세포술
Liver failure%Hepatitis B%Monocytes%Apoptosis%Cell proliferation%Flow cytometry
目的 研究免疫失衡状态下乙型肝炎慢加急性肝功能衰竭(ACLF)患者单核细胞在凋亡和抗原提呈方面的改变.方法 收集26例乙型肝炎ACLF患者、20例活动性慢性乙型肝炎(CHB)患者和18名健康对照者的外周血标本.应用流式细胞术检测单核细胞凋亡和增殖(Ki67)的改变,以及单核细胞表面抗原提呈分子人类白细胞抗原DR(HLA-DR)和B7分子(CD86)的表达水平.结果 ACLF组患者单核细胞膜联蛋白V的表达(64％)明显高于CHB组(28％)和健康对照组(20％),差异均有统计学意义(x2=11.75和27.23,P值均＜0.01),提示单核细胞凋亡增加.ACLF组单核细胞Ki67的表达(32％)低于健康对照组(61％)和CHB组(64％),差异均有统计学意义(x2=4.71和4.83,P值均＜0.05),提示活化的单核细胞减少.ACLF组患者单核细胞HLA-DR和CD86的平均荧光强度为22.85和11.63,明显低于健康对照组和CHB组,提示单核细胞抗原提呈能力受损.ACLF组死亡患者单核细胞膜联蛋白V阳性表达(63％)明显高于存活者(46％).结论 处于免疫失衡状态下的乙型肝炎ACLF患者外周血单核细胞的凋亡增加,活化细胞数量减少,从而导致单核细胞抗原提呈能力的下降.
目的 研究免疫失衡狀態下乙型肝炎慢加急性肝功能衰竭(ACLF)患者單覈細胞在凋亡和抗原提呈方麵的改變.方法 收集26例乙型肝炎ACLF患者、20例活動性慢性乙型肝炎(CHB)患者和18名健康對照者的外週血標本.應用流式細胞術檢測單覈細胞凋亡和增殖(Ki67)的改變,以及單覈細胞錶麵抗原提呈分子人類白細胞抗原DR(HLA-DR)和B7分子(CD86)的錶達水平.結果 ACLF組患者單覈細胞膜聯蛋白V的錶達(64％)明顯高于CHB組(28％)和健康對照組(20％),差異均有統計學意義(x2=11.75和27.23,P值均＜0.01),提示單覈細胞凋亡增加.ACLF組單覈細胞Ki67的錶達(32％)低于健康對照組(61％)和CHB組(64％),差異均有統計學意義(x2=4.71和4.83,P值均＜0.05),提示活化的單覈細胞減少.ACLF組患者單覈細胞HLA-DR和CD86的平均熒光彊度為22.85和11.63,明顯低于健康對照組和CHB組,提示單覈細胞抗原提呈能力受損.ACLF組死亡患者單覈細胞膜聯蛋白V暘性錶達(63％)明顯高于存活者(46％).結論 處于免疫失衡狀態下的乙型肝炎ACLF患者外週血單覈細胞的凋亡增加,活化細胞數量減少,從而導緻單覈細胞抗原提呈能力的下降.
목적 연구면역실형상태하을형간염만가급성간공능쇠갈(ACLF)환자단핵세포재조망화항원제정방면적개변.방법 수집26례을형간염ACLF환자、20례활동성만성을형간염(CHB)환자화18명건강대조자적외주혈표본.응용류식세포술검측단핵세포조망화증식(Ki67)적개변,이급단핵세포표면항원제정분자인류백세포항원DR(HLA-DR)화B7분자(CD86)적표체수평.결과 ACLF조환자단핵세포막련단백V적표체(64％)명현고우CHB조(28％)화건강대조조(20％),차이균유통계학의의(x2=11.75화27.23,P치균＜0.01),제시단핵세포조망증가.ACLF조단핵세포Ki67적표체(32％)저우건강대조조(61％)화CHB조(64％),차이균유통계학의의(x2=4.71화4.83,P치균＜0.05),제시활화적단핵세포감소.ACLF조환자단핵세포HLA-DR화CD86적평균형광강도위22.85화11.63,명현저우건강대조조화CHB조,제시단핵세포항원제정능력수손.ACLF조사망환자단핵세포막련단백V양성표체(63％)명현고우존활자(46％).결론 처우면역실형상태하적을형간염ACLF환자외주혈단핵세포적조망증가,활화세포수량감소,종이도치단핵세포항원제정능력적하강.
Objective To study apoptosis and antigen presentation changes of monocytes in HBV-related acute-on-chronic liver failure (ACLF) patients under immune dysfunction state.Methods Peripheral blood samples of 26 HBV-related ACLF patients (ACLF group),20 active chronic hepatitis B patients (CHB group) and 18 healthy individuals (control group) were collected.The changes of apoptosis and proliferation (Ki67) in monocytes and the expression of surface markers including human leukocyte antigen (HLA)-DR and B7 molecules (CD86) of monocytes were analyzed by flow cytometry. Results The percentage of Annexin V expressed monocytes of ACLF group (64％) was significantly higher than that of CHB group (28％) and control group (20％),and the difference was statistically significant (x2 value was 11.75 and 27.23 ; both P＜0.01),which indicated that monocytes apoptosis increased.The Ki67 expression in monocytes of ACLF group was lower than that of CHB group and control group,and the difference was statistically significant (x2 value was 4.71 and 4.83; both P＜ 0.05),which indicated that activated monocytes reduced. The mean fluorescence intensity (MFI) of HLA-DR and CD86 of monocytes in ACLF group was 22.85 and 11.63,which was significantly lower than that of CHB group and control group,indicating the antigen presentation ability of monocytes injured. The percentage of Annexin Ⅴ positive monocytes in survivals (62 ％ ) was significantly higher than that of dead patients (46 ％ ) in ACLF group.Conclusion In HBV-related ACLF patients under immune dysfunction state,the apoptosis of peripheral blood monocytes increased,and the quantity of activated cells reduced,resulting in the decline of the antigen presentation ability of monocytes.
