基础医学与临床
基礎醫學與臨床
기출의학여림상
BASIC MEDICAL SCIENCES AND CLINICS
2001年
1期
85-87
,共3页
阴彬%白龙川%袁建刚
陰彬%白龍川%袁建剛
음빈%백룡천%원건강
自身引物—模板PCR%串联体
自身引物—模闆PCR%串聯體
자신인물—모판PCR%천련체
建立“自身引物—模板”PCR方法,并应用此方法成功地扩增了多拷贝HIV-1 TAR片段的同向串联体。此方法简便,省力,可用于多拷贝基因探针、多拷贝反义基因片段、多拷贝抗原表位的扩增和克隆。
建立“自身引物—模闆”PCR方法,併應用此方法成功地擴增瞭多拷貝HIV-1 TAR片段的同嚮串聯體。此方法簡便,省力,可用于多拷貝基因探針、多拷貝反義基因片段、多拷貝抗原錶位的擴增和剋隆。
건립“자신인물—모판”PCR방법,병응용차방법성공지확증료다고패HIV-1 TAR편단적동향천련체。차방법간편,성력,가용우다고패기인탐침、다고패반의기인편단、다고패항원표위적확증화극륭。
Primer pair was designed as self-template during the amplification by PCR. It’s an easy way to construct a series of multi-copy genes. This method can be available for the amplification of DNA probes and the development of vaccines. It’s considered as a convenient and simple method for the cloning of multi-copy genes.