临床麻醉学杂志
臨床痳醉學雜誌
림상마취학잡지
THE JOURNAL OF CLINICAL ANESTHESIOLOGY
2009年
5期
431-432
,共2页
氟比洛芬酯%切口疼痛模型%反相高效液相法
氟比洛芬酯%切口疼痛模型%反相高效液相法
불비락분지%절구동통모형%반상고효액상법
Flurbiprofen axetil%Incision pain model%High peformanee liquid chromatography
目的 探讨氟比洛芬酯在大鼠手术切口组织中靶向分布,并探讨其可能的作用机制.方法 32只250 g左右的SD大鼠随机分为四组,每组8只.K2、K4、K8、K16组分别注入氟比洛芬酯2、4、8、16 mg/kg.使用反相高效液相法测定上清液中氟比洛芬的含量.结果 各组手术侧大鼠足底肌肉组织中氟比洛芬浓度均较未手术侧大鼠足底肌肉组织中氟比洛芬浓度明显升高(P<0.01),K16组最高.各组随着氟比洛芬酯给药量的增加,手术侧大鼠足底肌肉组织中氟比洛芬浓度也随着增高(P<0.05或P<0.01);未手术侧大鼠足底肌肉组织中氟比洛芬浓度K2、K4、K8组两两组间比较无统计学意义,但K16组未手术侧大鼠足底肌肉组织中氟比洛芬浓度与K2、K4、K8组两两组间比较有升高(P<0.01).结论 氟比洛芬酯在手术切口组织中具有一定的靶向分布;静脉注射较高剂量(16 mg/kg)的氟比洛芬酯时,大鼠正常肌肉组织中氟比洛芬的浓度显著升高.
目的 探討氟比洛芬酯在大鼠手術切口組織中靶嚮分佈,併探討其可能的作用機製.方法 32隻250 g左右的SD大鼠隨機分為四組,每組8隻.K2、K4、K8、K16組分彆註入氟比洛芬酯2、4、8、16 mg/kg.使用反相高效液相法測定上清液中氟比洛芬的含量.結果 各組手術側大鼠足底肌肉組織中氟比洛芬濃度均較未手術側大鼠足底肌肉組織中氟比洛芬濃度明顯升高(P<0.01),K16組最高.各組隨著氟比洛芬酯給藥量的增加,手術側大鼠足底肌肉組織中氟比洛芬濃度也隨著增高(P<0.05或P<0.01);未手術側大鼠足底肌肉組織中氟比洛芬濃度K2、K4、K8組兩兩組間比較無統計學意義,但K16組未手術側大鼠足底肌肉組織中氟比洛芬濃度與K2、K4、K8組兩兩組間比較有升高(P<0.01).結論 氟比洛芬酯在手術切口組織中具有一定的靶嚮分佈;靜脈註射較高劑量(16 mg/kg)的氟比洛芬酯時,大鼠正常肌肉組織中氟比洛芬的濃度顯著升高.
목적 탐토불비락분지재대서수술절구조직중파향분포,병탐토기가능적작용궤제.방법 32지250 g좌우적SD대서수궤분위사조,매조8지.K2、K4、K8、K16조분별주입불비락분지2、4、8、16 mg/kg.사용반상고효액상법측정상청액중불비락분적함량.결과 각조수술측대서족저기육조직중불비락분농도균교미수술측대서족저기육조직중불비락분농도명현승고(P<0.01),K16조최고.각조수착불비락분지급약량적증가,수술측대서족저기육조직중불비락분농도야수착증고(P<0.05혹P<0.01);미수술측대서족저기육조직중불비락분농도K2、K4、K8조량량조간비교무통계학의의,단K16조미수술측대서족저기육조직중불비락분농도여K2、K4、K8조량량조간비교유승고(P<0.01).결론 불비락분지재수술절구조직중구유일정적파향분포;정맥주사교고제량(16 mg/kg)적불비락분지시,대서정상기육조직중불비락분적농도현저승고.
Objective To study target-distribution of flurbiprofen axetil in operative incision tissue in incision-induced rats. Methods Thirty-two-250 g-weight rats were randomly divided into 4 groups. The incision pain model was established by being operated according to Brennan's method. Two hours after vena caudalis injection, all the rats were anesthetized deeply by pentobarbital sodium-perito injection 100 mg/kg,muscles of both hind paws were dissected, homogenated, centrifuged and supernatant fluids were dissociate. The concentration of flurbiprofen were detected by reversed phase high peformance liquid chromatography(RP-HPLC). Results In these groups of different dosage, the concentration of flurbiprofen in operative incision notably increased compared to that in the non-operative incision, especially in group K16. The concentration of flurbiprofen in operative incision of different dosage increased in dose-dependent manner. The difference of concentration of flurbiprofen in non-operative incisions of K2, K4, K8 was statistically insignificant, but the concentration of flurbiprofen in non-operative incision of K6 increased compared to that of K2, K4 and K8. Conclusion The distribution of flurbiprofen axeti in operative incision was targeted. When rats were injected flurbiprofen axetil at 16 mg/kg by vena caudalis, The concentration of flurbiprofen in the non-operative incision increased notably.
