CAJ | 학술논문

从连云港海滩晒虾蟹壳的泥土里筛选出一株产壳聚糖酶能力较高的菌株S-1(水解圈直径和菌落直径的比值高达12.5).根据其形态特征、生理生化以及16S rDNA鉴定,初步确定该菌为芽孢杆菌属(Bacillus);采用单因子试验方法,确定该菌的最佳培养基配方为(g/L):虾蟹壳粉15、硝酸铵10、酵母提取物3、K2HPO4·3H2O1.4、NaCl 5、MgSO4·7H2O 1.4、葡萄糖1.0:利用正交试验进行发酵条件的优化,确定最适宜发酵条件为初始pH 6.0,32℃,用500ml三角瓶装液量为100ml,发酵时间为72h,最终该菌的酶活力由复筛的2.13U/ml提高到6.4U/ml,从而使该菌株具有工业化应用的潜力.
종련운항해탄쇄하해각적니토리사선출일주산각취당매능력교고적균주S-1(수해권직경화균락직경적비치고체12.5).근거기형태특정、생리생화이급16S rDNA감정,초보학정해균위아포간균속(Bacillus);채용단인자시험방법,학정해균적최가배양기배방위(g/L):하해각분15、초산안10、효모제취물3、K2HPO4·3H2O1.4、NaCl 5、MgSO4·7H2O 1.4、포도당1.0:이용정교시험진행발효조건적우화,학정최괄의발효조건위초시pH 6.0,32℃,용500ml삼각병장액량위100ml,발효시간위72h,최종해균적매활력유복사적2.13U/ml제고도6.4U/ml,종이사해균주구유공업화응용적잠력.
Twenty chitosanase-preducing bacterium swains were isolated from Lianyungang seaside. Among them strain S-1 had the highest chitosanase-producing capacity and was identified as Bacillus sp. based on its morphological, physiological and biochemical properties as well as 16S rDNA sequence analysis. Single-factor method and orthogonal array design were adopted to optimize the medium composition and fermentation parameters to maximize chitosanase activity, respectively. The optimal medium for enzyme production consisted of 15 g/L shrimp- and crab-shell powder, 10 g/L NH4NO3, 3 g/L yeast extract, 1.4 g/L 100 ml in 500 ml flask. After cultivation at 32 ℃ for 72 h, the maximal chitosanase activity was observed to be 6.4 U/ml. This strain has promising potential in the industrial production of chitosanase.