亚热带植物科学
亞熱帶植物科學
아열대식물과학
SUBTROPICAL PLANT SCIENCE
2009年
4期
62-65
,共4页
达摩兰%茎段%组织培养%外植体
達摩蘭%莖段%組織培養%外植體
체마란%경단%조직배양%외식체
dharma orchid%stem%tissue culture%explant
以达摩兰的原种苗茎段为外植体进行组织培养研究,筛选出最适培养基(1)原球茎诱导:MS + 6-BA 0.5 mg/L + NAA 1.0 mg/L + 香蕉肉250 g/L;(2)丛生芽诱导:1/2MS + 6-BA 3 mg/L + NAA 2.0 mg/L + AC 2.5 g/L + 蔗糖3% + 香蕉肉200 g/L;(3)芽增殖:1/2MS + 6-BA 3 mg/L + NAA 2.0 mg/L + AC 2.5 g/L + 蔗糖3% + 香蕉肉150 g/L;(4)根诱导:1/2MS + 6-BA 0.3 mg/L + IBA 0.5 mg/L + NAA 0.5 mg/L + AC 2.5 g/L + 蔗糖3%.采用珍珠岩、细石与树皮的混合物为培养基质,移栽成活率达95%以上.
以達摩蘭的原種苗莖段為外植體進行組織培養研究,篩選齣最適培養基(1)原毬莖誘導:MS + 6-BA 0.5 mg/L + NAA 1.0 mg/L + 香蕉肉250 g/L;(2)叢生芽誘導:1/2MS + 6-BA 3 mg/L + NAA 2.0 mg/L + AC 2.5 g/L + 蔗糖3% + 香蕉肉200 g/L;(3)芽增殖:1/2MS + 6-BA 3 mg/L + NAA 2.0 mg/L + AC 2.5 g/L + 蔗糖3% + 香蕉肉150 g/L;(4)根誘導:1/2MS + 6-BA 0.3 mg/L + IBA 0.5 mg/L + NAA 0.5 mg/L + AC 2.5 g/L + 蔗糖3%.採用珍珠巖、細石與樹皮的混閤物為培養基質,移栽成活率達95%以上.
이체마란적원충묘경단위외식체진행조직배양연구,사선출최괄배양기(1)원구경유도:MS + 6-BA 0.5 mg/L + NAA 1.0 mg/L + 향초육250 g/L;(2)총생아유도:1/2MS + 6-BA 3 mg/L + NAA 2.0 mg/L + AC 2.5 g/L + 자당3% + 향초육200 g/L;(3)아증식:1/2MS + 6-BA 3 mg/L + NAA 2.0 mg/L + AC 2.5 g/L + 자당3% + 향초육150 g/L;(4)근유도:1/2MS + 6-BA 0.3 mg/L + IBA 0.5 mg/L + NAA 0.5 mg/L + AC 2.5 g/L + 자당3%.채용진주암、세석여수피적혼합물위배양기질,이재성활솔체95%이상.
The trial used the original seedling stems of dharma orchid for tissue culture technology study, selected out the optimum medium:(1) protocorm induced: MS + 6-BA 0.5 mg/L + NAA 1.0 mg/L + banana 250 g/L;(2) bud induced: 1/2 MS + 6-BA 3 mg/L + NAA2.0 mg/L + AC 2.5 mg/L + su 3% + banana 200g/L;(3) shoot proliferation: 1/2 MS + 6-BA3 mg/L + NAA 2.0 mg/L + AC 2.5 mg/L + su 3% + banana 150g/L;(4) rooting: 1/2 MS + 6-BA 0.3 mg/L + IBA 0.5 mg/L+NAA 0.5 mg/L + AC 2.5 mg/L + su 3%.Using mixture of perlite, fine stone and bark as culture substratum, the survival rate was up to 95%.
