华北农学报
華北農學報
화북농학보
ACTA AGRICULTURAE BOREALI-SINICA
2009年
z2期
176-179
,共4页
过氧化物酶%酯酶%淀粉酶%远缘杂交%优势组合
過氧化物酶%酯酶%澱粉酶%遠緣雜交%優勢組閤
과양화물매%지매%정분매%원연잡교%우세조합
Peroxidase%Esterase%Amylase%Distant hybridization%Superiority combination
本试验探索了甘薯过氧化物酶、酯酶、淀粉酶同工酶电泳的最佳条件,利用这3种同工酶对本课题组保存的优势组合甘薯野生材料Y-6和栽培品种冀21-2及其由其杂交选育出的系列品种(品系)冀98、冀99、冀79、冀71、冀52进行深人的分析,结果显示:优势组合Y-6与21-2的杂交后代中出现了杂种优势的特征酶谱类型--杂种型、偏亲本型等等,各材料生产上的实际表现证实了同工酶分析的可靠性;表明:远缘组合Y-6与冀21-2属于强优势组合,并且同工酶分析技术可以用于筛选鉴定甘薯优势组合及其杂交后代早期辅助选择,加快育种进程.
本試驗探索瞭甘藷過氧化物酶、酯酶、澱粉酶同工酶電泳的最佳條件,利用這3種同工酶對本課題組保存的優勢組閤甘藷野生材料Y-6和栽培品種冀21-2及其由其雜交選育齣的繫列品種(品繫)冀98、冀99、冀79、冀71、冀52進行深人的分析,結果顯示:優勢組閤Y-6與21-2的雜交後代中齣現瞭雜種優勢的特徵酶譜類型--雜種型、偏親本型等等,各材料生產上的實際錶現證實瞭同工酶分析的可靠性;錶明:遠緣組閤Y-6與冀21-2屬于彊優勢組閤,併且同工酶分析技術可以用于篩選鑒定甘藷優勢組閤及其雜交後代早期輔助選擇,加快育種進程.
본시험탐색료감서과양화물매、지매、정분매동공매전영적최가조건,이용저3충동공매대본과제조보존적우세조합감서야생재료Y-6화재배품충기21-2급기유기잡교선육출적계렬품충(품계)기98、기99、기79、기71、기52진행심인적분석,결과현시:우세조합Y-6여21-2적잡교후대중출현료잡충우세적특정매보류형--잡충형、편친본형등등,각재료생산상적실제표현증실료동공매분석적가고성;표명:원연조합Y-6여기21-2속우강우세조합,병차동공매분석기술가이용우사선감정감서우세조합급기잡교후대조기보조선택,가쾌육충진정.
This paper explored the best condition of the POD, EST, AMY, and analysised the wild sweetpotato Y-6, Ji21-2 and its five hybrid progenies including Ji98, Ji99, Ji79, Ji71 ,Ji52, its result showed that the reliability of the isozymes analysis in sweetpotato was validated from two aspects of existing zymogram types of the heterosis characteristics in the filial generation, such as the hybrid type, paternal or maternal type, combined with the performance of corresponding euhivar in the production. The cross combination wild sweetpotato Y-6 Ji21-2 belongs to the strong heterosis one, and the isozyme technique can be used to screen the strong combination and the early generation of the filial generation, and speeding up the breeding process.