国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2008年
18期
1106-1108
,共3页
60Coγ照射%深低温保存%大鼠同种带瓣主肺动脉%CD4+T细胞%CD8+T细胞%免疫原性
60Coγ照射%深低溫保存%大鼠同種帶瓣主肺動脈%CD4+T細胞%CD8+T細胞%免疫原性
60Coγ조사%심저온보존%대서동충대판주폐동맥%CD4+T세포%CD8+T세포%면역원성
60Co γ irradiation%Cryopreservation%Rat valved pulmonary homograft conduits%CD4+ Tcell%CD8+ T cell%Immunogenicity
目的 本实验应用60Co γ射线照射加液氮深低温保存大鼠同种带瓣主肺动脉(valvedpulmonary homograft conduits,VPHC),并建立大鼠VPHC颈总动脉移植模型,观察不同照射剂量对同种带瓣主肺动脉免疫原性的作用,寻找合适照射剂量,为进一步基础研究及临床应用提供理论依据.方法 1月龄封闭群Wistar大鼠50只作为供体鼠,雌雄不拘,随机分为单纯深低温保存组(实验对照组)、300 Gy照射组、600 Gy照射组、900 Gy照射组和1200 Gy照射组;成年雄性SD大鼠50只作为受体鼠,随机分为5组;另取10只SD大鼠行颈总动脉切断吻合术,作为空白对照组.应用流式细胞术对VPHC移植术后1周、2周、3周受体鼠外周血行CD4+T细胞及CD8+T细胞检测.结果 照射组VPHC移植术后1周、2周、3周CD4+T细胞、CD8+T细胞百分率及CD4+/CD8+与空白对照组相比差异有统计学意义(P<0.05),与实验对照组相比差异有统计学意义(P<0.05).结论 应用60Co γ对VPHC进行照射在保持VPHC活性的同时明显降低了其免疫原性.
目的 本實驗應用60Co γ射線照射加液氮深低溫保存大鼠同種帶瓣主肺動脈(valvedpulmonary homograft conduits,VPHC),併建立大鼠VPHC頸總動脈移植模型,觀察不同照射劑量對同種帶瓣主肺動脈免疫原性的作用,尋找閤適照射劑量,為進一步基礎研究及臨床應用提供理論依據.方法 1月齡封閉群Wistar大鼠50隻作為供體鼠,雌雄不拘,隨機分為單純深低溫保存組(實驗對照組)、300 Gy照射組、600 Gy照射組、900 Gy照射組和1200 Gy照射組;成年雄性SD大鼠50隻作為受體鼠,隨機分為5組;另取10隻SD大鼠行頸總動脈切斷吻閤術,作為空白對照組.應用流式細胞術對VPHC移植術後1週、2週、3週受體鼠外週血行CD4+T細胞及CD8+T細胞檢測.結果 照射組VPHC移植術後1週、2週、3週CD4+T細胞、CD8+T細胞百分率及CD4+/CD8+與空白對照組相比差異有統計學意義(P<0.05),與實驗對照組相比差異有統計學意義(P<0.05).結論 應用60Co γ對VPHC進行照射在保持VPHC活性的同時明顯降低瞭其免疫原性.
목적 본실험응용60Co γ사선조사가액담심저온보존대서동충대판주폐동맥(valvedpulmonary homograft conduits,VPHC),병건립대서VPHC경총동맥이식모형,관찰불동조사제량대동충대판주폐동맥면역원성적작용,심조합괄조사제량,위진일보기출연구급림상응용제공이론의거.방법 1월령봉폐군Wistar대서50지작위공체서,자웅불구,수궤분위단순심저온보존조(실험대조조)、300 Gy조사조、600 Gy조사조、900 Gy조사조화1200 Gy조사조;성년웅성SD대서50지작위수체서,수궤분위5조;령취10지SD대서행경총동맥절단문합술,작위공백대조조.응용류식세포술대VPHC이식술후1주、2주、3주수체서외주혈행CD4+T세포급CD8+T세포검측.결과 조사조VPHC이식술후1주、2주、3주CD4+T세포、CD8+T세포백분솔급CD4+/CD8+여공백대조조상비차이유통계학의의(P<0.05),여실험대조조상비차이유통계학의의(P<0.05).결론 응용60Co γ대VPHC진행조사재보지VPHC활성적동시명현강저료기면역원성.
Objective The method of keeping valved pulmonary homograft conduits(VPHC) by using 60Co γ irradiation and cryopreservation is used in this experiment and the transplantation model of VPHC is built. Different effects to the immunogenicity in VPHC rat caused by different dosage of 60 Co iracliation are observed. To determine the suitable dosage of 60 Co γ used prior to cryopreservation in order to reduce the immunogenicity yet maintain the viability of VPHC. And to provide academic basis for further basic research and clinical application. Methods Fifty one-month old Wistar rats were used as donors and were divided into five groups (randomly,leave out sex ). Fifty SD adult rats were used as recipients and were divided into five groups respectively. Four experimental groups were irradiated with 60 Co γ of different dosages prior to cryopreservation:300 Gy group, 600 Gy group, 900 Gy group, 1200 Gy group. The experimental control group received cryopreservation only. And ten SD rats underwent right carotid artery end-to-end anastomosis were used as a control group. Flow cytometry was used to test CD4+ ,CD8+ T cells in peripheral blood of recipient rats at weekly interval of 1,2, 3 weeks postoperatively. Results There is significant difference between experimental group and control group in percentages of CD4+ , CD8+ T cells and CD4+/CD8+ ( P<0.05). Conclusions 60 Co γ irradiation can keep the activity of VPHC while reduce immunogenicity of VPHC at the same time in rats.
