CAJ | 학술논문

目的探讨早幼粒细胞白血病蛋白(PML)和P53在食管鳞状细胞癌(ESCC)及癌前病变发生发展中的作用.方法应用组织芯片技术和免疫组织化学方法检测PML和P53在241例ESCC及癌前病变中的差异表达,分析蛋白不同表达模式与临床病理参数的相关性.结果在ESCC及癌前病变中,PML和P53呈阳性或强阳性表达,而在正常食管鳞状黏膜上皮中,呈阴性或仅在副基底层细胞有少量阳性表达.PML表达水平与淋巴结转移(X2=15.226,P<0.001)、浸润深度(X2=29.461,P<0.001)及临床分期(X2=26.956,P<O.001)等有关.结论PML和P53在ESCC及癌前病变的发生发展中有重要作用.PML可作为一新的生物标记物,在ESCC及癌前病变的发生和演进中具有预警作用.
목적탐토조유립세포백혈병단백(PML)화P53재식관린상세포암(ESCC)급암전병변발생발전중적작용.방법응용조직심편기술화면역조직화학방법검측PML화P53재241례ESCC급암전병변중적차이표체,분석단백불동표체모식여림상병리삼수적상관성.결과재ESCC급암전병변중,PML화P53정양성혹강양성표체,이재정상식관린상점막상피중,정음성혹부재부기저층세포유소량양성표체.PML표체수평여림파결전이(X2=15.226,P<0.001)、침윤심도(X2=29.461,P<0.001)급림상분기(X2=26.956,P<O.001)등유관.결론PML화P53재ESCC급암전병변적발생발전중유중요작용.PML가작위일신적생물표기물,재ESCC급암전병변적발생화연진중구유예경작용.
Objective To assess the role of promyelocytic leukemia protein (PML)and P53 in the progression of esophageal squamous cell carcinoma(ESCC)and its precursor lesions. Methods Different expression patterns of PML and P53 of 241 cases of ESCC combined with adjacent precursors were analyzed by tissue array and immunohistochemistry and correlated with clinicopathological parameters. Results In ESCC and its precursor lesions, PML and P53 displayed positive or strong positive, while in normal esophageal epithelia, these proteins showednegative or stained positive only in parabasal cell layer. The expression level of PML was correlated with the depth of invasion of esophageal carcinomas (X2=29.461,P<0.001),lymph metastasis status(X2=15.226,P<0.001)and pTNMs(x2=26.956,P<o.001).Conclusion The abnormal expression levels of PML and P53 may play important roles in the carcinogenesis and evolution of ESCC and its precursors. The result suggests that PML, as a novel molecular biomarker, may be used to predict the development and progression of ESCC and its precursors.