中国地方病学杂志
中國地方病學雜誌
중국지방병학잡지
CHINESE JOURNAL OF ENDEMIOLOGY
2010年
4期
443-445
,共3页
秦迎旭%高建炜%田涛%马学平%卢世堂%张生龙%朱勇%孙学东%薛向阳%伏卫程%王红娅
秦迎旭%高建煒%田濤%馬學平%盧世堂%張生龍%硃勇%孫學東%薛嚮暘%伏衛程%王紅婭
진영욱%고건위%전도%마학평%로세당%장생룡%주용%손학동%설향양%복위정%왕홍아
鼠疫%抗体%血清流行病学研究
鼠疫%抗體%血清流行病學研究
서역%항체%혈청류행병학연구
Plague%Antibodies%Seroepidemiologic studies
目的 了解2007年和2008年宁夏鼠疫自然疫源地人群鼠疫F1抗体水平和分布情况.方法 采集宁夏鼠疫发生地和动物鼠疫流行疫源地的5个市(县)居民血样718份作为调查对象,采集非疫区居民血样475份作为对照组.用间接血凝试验、胶体金试验、酶联免疫吸附试验3种方法检测人群鼠疫F1抗体.两种以上方法检测为阳性者,判定为阳性血样;抗体滴度没有达到阳性判定标准者,判定为可疑血样.结果 检测718份血样,检出阳性血样9份(滴度在1:16～1:64),阳性率为1.25%(9/718);可疑血样28份,检出率为3.90%(28/718).3种方法检测非疫区人群血样475份,全部为阴性.疫区和非疫区人群血清鼠疫F1抗体阳性率比较,差异有统计学意义(χ2=4.44,P<0.05).间接血凝试验、胶体金试验、酶联免疫吸附试验3种检测方法的阳性率[1.25%(9/718)、1.25%(9/718)、2.51%(18/718)]比较,差异无统计学意义(χ2=1.91,P>0.05).结论 宁夏鼠疫自然疫源地内尚存在一定比例的鼠疫F1抗体阳性人群,均分布在近几年有动物鼠疫流行的地区.
目的 瞭解2007年和2008年寧夏鼠疫自然疫源地人群鼠疫F1抗體水平和分佈情況.方法 採集寧夏鼠疫髮生地和動物鼠疫流行疫源地的5箇市(縣)居民血樣718份作為調查對象,採集非疫區居民血樣475份作為對照組.用間接血凝試驗、膠體金試驗、酶聯免疫吸附試驗3種方法檢測人群鼠疫F1抗體.兩種以上方法檢測為暘性者,判定為暘性血樣;抗體滴度沒有達到暘性判定標準者,判定為可疑血樣.結果 檢測718份血樣,檢齣暘性血樣9份(滴度在1:16～1:64),暘性率為1.25%(9/718);可疑血樣28份,檢齣率為3.90%(28/718).3種方法檢測非疫區人群血樣475份,全部為陰性.疫區和非疫區人群血清鼠疫F1抗體暘性率比較,差異有統計學意義(χ2=4.44,P<0.05).間接血凝試驗、膠體金試驗、酶聯免疫吸附試驗3種檢測方法的暘性率[1.25%(9/718)、1.25%(9/718)、2.51%(18/718)]比較,差異無統計學意義(χ2=1.91,P>0.05).結論 寧夏鼠疫自然疫源地內尚存在一定比例的鼠疫F1抗體暘性人群,均分佈在近幾年有動物鼠疫流行的地區.
목적 료해2007년화2008년저하서역자연역원지인군서역F1항체수평화분포정황.방법 채집저하서역발생지화동물서역류행역원지적5개시(현)거민혈양718빈작위조사대상,채집비역구거민혈양475빈작위대조조.용간접혈응시험、효체금시험、매련면역흡부시험3충방법검측인군서역F1항체.량충이상방법검측위양성자,판정위양성혈양;항체적도몰유체도양성판정표준자,판정위가의혈양.결과 검측718빈혈양,검출양성혈양9빈(적도재1:16～1:64),양성솔위1.25%(9/718);가의혈양28빈,검출솔위3.90%(28/718).3충방법검측비역구인군혈양475빈,전부위음성.역구화비역구인군혈청서역F1항체양성솔비교,차이유통계학의의(χ2=4.44,P<0.05).간접혈응시험、효체금시험、매련면역흡부시험3충검측방법적양성솔[1.25%(9/718)、1.25%(9/718)、2.51%(18/718)]비교,차이무통계학의의(χ2=1.91,P>0.05).결론 저하서역자연역원지내상존재일정비례적서역F1항체양성인군,균분포재근궤년유동물서역류행적지구.
Objective To understand the level and distribution of antibody F1 against plague in population of Ningxia natural plague foci in 2007 and 2008. Methods Seven hundred and eighteen blood samples were collected in five major cities and counties of natural plague foci, and 475 blood samples were collected in nonplague area as control group. Conventional indirect hemagglutination, colloidal gold test, and enzyme-linked immunoassay were employed to test the antibody. If the result was tested positive by more than two methods used then the result was defined as positive. Antibody titer that did not reach the positive standard was defined as suspected samples. Results A total of 718 serum samples were tested, the results showed that 9 samples were positive (antibody titer was 1:16 - 1:64), the positive rate was 1.25%(9/718), suspected samples was 28, the detection rate was 3.90%(28/718). Four hundred and seventy-five serum samples in the non-plague area were all negative by the three methods. There was a significant difference of antibody F1 positive rate between residents in historical epidemic area and history nonepidemic area(χ2 = 4.44, P< 0.05). There was no statistical significance of the positive rate[1.25%(9/718), 1.25%(9/718),2.51%(18/718)]among the three methods used(χ2 = 1.91, P> 0.05). Conclusion There still exists a certain proportion of Fl antibody positive people in Ningxia natural plague foci, and these people are distributed in areas where several animal plague prevalent in recent years.
