CAJ | 학술논문

目的探讨新疆维吾尔族人群HLA-DRB1等位基因多态性与HBV感染临床转归及HBV不同复制状态的相关性.方法采用序列特异性引物-PCR方法对70例慢性乙型肝炎患者(CHB组)、46例HBV携带者(携带组)、42例HBV感染后自然恢复者(恢复组)、80名健康献血员(对照组)进行HLA-DRB1等位基因分型比较,并比较HBV不同复制状态下DRB1多态性的分布频率及其差异.结果 HBV感染后恢复组与对照组HLA-DRB1* 0701等位基因频率分别为13.09％和13.75％,均分别高于CHB组(5.71％)及携带组(4.35％),差异有统计学意义(x2=4.06、6.03,P＜0.05,OR =0.36、0.34; x2=4.75、6.31,P＜0.05,OR=0.27、0.25)；恢复组DRB1* 1201-3等位基因频率16.67％,高于CHB组(7.86％),差异有统计学意义(x2=4.70,P＜0.05,OR=0.37)；DRB1* 1301-2基因在对照组和恢复组的频率分别9.38％、10.70％,显著高于CHB组,差异有统计学意义(x2=14.58、13.54,P＜0.01)；其他各等位基因频率在各组间差异无统计学意义；HBV不同复制状态HLA-DRB1等位基因分布频率差异无统计学意义(P＞0.05).结论新疆维吾尔族携带HLA-DRB1* 0701、DRB1* 1201-3、DRB1*1301-2基因型可能降低慢性乙型肝炎发生的风险,是HBV感染的抗性基因.
목적 탐토신강유오이족인군HLA-DRB1등위기인다태성여HBV감염림상전귀급HBV불동복제상태적상관성.방법 채용서렬특이성인물-PCR방법대70례만성을형간염환자(CHB조)、46례HBV휴대자(휴대조)、42례HBV감염후자연회복자(회복조)、80명건강헌혈원(대조조)진행HLA-DRB1등위기인분형비교,병비교HBV불동복제상태하DRB1다태성적분포빈솔급기차이.결과 HBV감염후회복조여대조조HLA-DRB1* 0701등위기인빈솔분별위13.09％화13.75％,균분별고우CHB조(5.71％)급휴대조(4.35％),차이유통계학의의(x2=4.06、6.03,P＜0.05,OR =0.36、0.34; x2=4.75、6.31,P＜0.05,OR=0.27、0.25)；회복조DRB1* 1201-3등위기인빈솔16.67％,고우CHB조(7.86％),차이유통계학의의(x2=4.70,P＜0.05,OR=0.37)；DRB1* 1301-2기인재대조조화회복조적빈솔분별9.38％、10.70％,현저고우CHB조,차이유통계학의의(x2=14.58、13.54,P＜0.01)；기타각등위기인빈솔재각조간차이무통계학의의；HBV불동복제상태HLA-DRB1등위기인분포빈솔차이무통계학의의(P＞0.05).결론 신강유오이족휴대HLA-DRB1* 0701、DRB1* 1201-3、DRB1*1301-2기인형가능강저만성을형간염발생적풍험,시HBV감염적항성기인.
Objective To investigate the associations of human leukocyte antigen (HLA)-DRB1 polymorphism with the outcome of HBV infection and the replication of HBV in Uygur population of Xinjiang.Methods Polymerase chain reaction-sequence specific primers (PCR-SSP) was used to genotype HLA-DRB1 gene.70 chronic hepatitis B patients (CHB group),46 chronic asymptomatic hepatitis B virus carriers( carrier groups),42 spontaneous rehabilitee after HBV infection(rehabilitee group)and 80 healthy controls were recruited.The frequency distributions of genotype among four groups were analyzed,and the frequency of that in different replication of HBV was also studied.Results The frequency of HLA-DRB1 * 0701 allele in rehabilitee group( 13.09％ ) and control group (13.75 ％ ) were significantly higher than that in CHB group(5.71％) (x2 =4.06,6.03,P ＜ 0.05,OR =0.36,0.34) and in carriers group (4.35％)( x2 =4.75,6.31,P ＜ 0.05,OR =0.27,0.25).The frequency of HLA-DRB1 * 1201-3 allele in rehabilitee group was higher than that in CHB group (16.67％:7.86％,x2 =4.70,P ＜0.05,OR =0.37).The frequencies of HLA- DRB1 * 1301-2 in control group and rehabilitee group were 9.38 ％ and 10.7 ％,significantly higher than that in CHB group( x2 =13.54,14.58,P ＜ 0.01).The frequencies of other DRB1 alleles were no significant differences among the groups,and the same result was observed in different replication of HBV( P ＞ 0.05).Conclusions Alleles of HLA-DRB1 * 0701,DRB1 * 1201-3 and DRB1 * 1301-2 are closely associated with HBV clearance,which may be the resistant genes for CHB in Uygur population of Xinjiang.