CAJ | 학술논문

目的观察昆布多糖对卵清蛋白(OVA)致敏激发哮喘小鼠气道炎症及重塑的影响.方法将32只昆明系小鼠被随机分为模型组、激素吸入组、昆布多糖组和对照组4组,每组8只.经OVA致敏激发制模成功后,模型组用生理盐水0.3 ml灌胃干预,激素吸入组雾化吸入布地奈德混悬液0.4 ml(200 μg)+生理盐水3.6 ml干预,昆布多糖组采用昆布多糖50 mg/kg灌胃干预,均每日1次.对照组采用生理盐水代替OVA致敏激发和灌胃干预.各组动物于42 d后麻醉,行右肺支气管肺泡灌洗留取灌洗液(BALF),并取右肺组织进行病理学观察.在光镜下计数BALF中细胞总数及分类;采用酶联免疫吸附法(ELISA)检测BALF中γ-干扰素(IFN-γ)、白细胞介素-4(IL-4)水平;肺组织病理学改变程度在苏木素-伊红(HE)染色后于光镜下进行评价;肺组织基质金属蛋白酶-9(MMP-9)及金属蛋白酶组织抑制剂-1(TIMP-1)的表达采用免疫组化法检测.结果与模型组比较,昆布多糖组及激素吸入组BALF中细胞总数及分类计数、IL-4、肺组织病理学评分均显著降低,IFN-γ显著升高(P均<0.01);激素吸入组MMP-9、TIMP-1及昆布多糖组TIMP-1均显著下降(P<0.05或P<0.01);与对照组比较,昆布多糖组各指标差异仍有统计学意义(P<0.05或P<0.01);与昆布多糖组比较,激素吸入组细胞总数及分类计数、IFN-γ、病理学评分、MMP-9、TIMP-1差异均有统计学意义(P<0.05或P<0.01).结论昆布多糖在一定程度上能抑制急性哮喘小鼠的气道炎症,减轻气道重塑的发生,可能是一种新的治疗哮喘的辅助药物.
목적 관찰곤포다당대란청단백(OVA)치민격발효천소서기도염증급중소적영향.방법 장32지곤명계소서피수궤분위모형조、격소흡입조、곤포다당조화대조조4조,매조8지.경OVA치민격발제모성공후,모형조용생리염수0.3 ml관위간예,격소흡입조무화흡입포지내덕혼현액0.4 ml(200 μg)+생리염수3.6 ml간예,곤포다당조채용곤포다당50 mg/kg관위간예,균매일1차.대조조채용생리염수대체OVA치민격발화관위간예.각조동물우42 d후마취,행우폐지기관폐포관세류취관세액(BALF),병취우폐조직진행병이학관찰.재광경하계수BALF중세포총수급분류;채용매련면역흡부법(ELISA)검측BALF중γ-간우소(IFN-γ)、백세포개소-4(IL-4)수평;폐조직병이학개변정도재소목소-이홍(HE)염색후우광경하진행평개;폐조직기질금속단백매-9(MMP-9)급금속단백매조직억제제-1(TIMP-1)적표체채용면역조화법검측.결과 여모형조비교,곤포다당조급격소흡입조BALF중세포총수급분류계수、IL-4、폐조직병이학평분균현저강저,IFN-γ현저승고(P균<0.01);격소흡입조MMP-9、TIMP-1급곤포다당조TIMP-1균현저하강(P<0.05혹P<0.01);여대조조비교,곤포다당조각지표차이잉유통계학의의(P<0.05혹P<0.01);여곤포다당조비교,격소흡입조세포총수급분류계수、IFN-γ、병이학평분、MMP-9、TIMP-1차이균유통계학의의(P<0.05혹P<0.01).결론 곤포다당재일정정도상능억제급성효천소서적기도염증,감경기도중소적발생,가능시일충신적치료효천적보조약물.
Objective To investigate the effect of okam (昆布) on inflammation and remodeling of airway in mice with ovalbumin (OVA) induced asthma. Methods Thirty-two mice of Kunming strain were divided into four groups randomly: model group, glucocorticoid inhalation group, okam group and control group, with 8 mice in each group. The asthmatic mice model was reproduced by combined injection and aerosol inhalation of OVA. The mice in model group received normal saline (0. 3 ml) gavage daily. The mice in glucocorticoid inhalation group received budesonide (0. 4 ml, 200 μg) and normal saline (3.6 ml) inhalation. The mice in okam group were gavaged with okam daily (50 mg/kg). The controls were given normal saline instead of OVA sensitization. All mice were sacrificed 42 days later, followed by lavage of tracheo-bronchial tree of the right lung, and the right lung was saved for pathological examination. The total cell number and differentiation in bronchoalveolar lavage fluid (BALF) were counted under microscope. The expression of interferon-γ (IFN-γ), interleukin-4 (IL-4) in BALF were assessed by enzyme linked immunosorbent asay (ELISA). The histological changes in the bronchi and alveoli were evaluated after hematoxylin and eosin (HE) staining. The expression of matrix metalloproteinase-9 (MMP-9) as well as the tissue inhibitor of metalloproteinase-1 (TIMP-1) were determined by immunohistochemistry. Results Compared with the model group, the total cell count and IL-4 level in BALF, and the score of pathological changes in the broncho-alveolar tissue in okam group or glucocorticoid inhalation group were lower significantly, and the IFN-γ level elevated markedly (all P ＜0.01). The MMP-9, TIMP-1 expression in glucocorticoid inhalation group and the TIMP-1 expression in okam group were decreased greatly (P＜0. 05 or P＜0. 01). All of above indexes showed marked differences between control group and okam group (P＜ 0. 05 or P＜0. 01). There were significant changes in the total cell count, IFN-γ, pathological changes, MMP-9 and TIMP-1 between the glucocorticoid inhalation group and the okam group (P＜0. 05 or P＜ 0. 01). Conclusion Okam may alleviate inflammation of the bronchia and degrade the development of airway remodeling to some degree.