中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2010年
4期
203-206
,共4页
邓辉%吴亚菲%丁一%苗棣%高雳%郭淑娟
鄧輝%吳亞菲%丁一%苗棣%高靂%郭淑娟
산휘%오아비%정일%묘체%고력%곽숙연
牙周炎%内皮细胞%细菌黏附%侵入
牙週炎%內皮細胞%細菌黏附%侵入
아주염%내피세포%세균점부%침입
Periodontitis%Endothelial cells%Bacterial adhesion%Invasion
目的 研究和比较4种常见牙周致病菌Pg33277、Pi25611、Aa29522和Fn10953黏附和侵入人脐静脉内皮细胞(human umbilical vein endothelial cells,HUVEC)的能力,为深入探讨牙周致病菌感染HUVEC在促进动脉粥样硬化发生、发展中的作用奠定初步基础.方法 建立牙周致病菌感染HUVEC的体外模型,采用扫描电镜、抗生素保护-菌落计数法观察以上牙周致病菌黏附和侵入HUVEC的能力.结果扫描电镜结果表明Pg33277、Pi25611、Aa29522和Fn10953均可黏附于HUVEC;抗生素保护法结果发现Pg33277、Pi25611、Aa29522和Fn10953均可侵入HUVEC,细菌侵入量分别为(0.8±0.1)×10~8、(4.1±0.5)×10~6、(1.6±0.3)×10~6、(5.0±0.4)×10~6CFU/L,侵入率分别为(0.400±0.050)%、(0.021±0.003)%、(0.008±0.002)%和(0.025±0.002)%,Pg33277侵入能力远较其他3种牙周致病菌强(P＜0.001),其余3种牙周致病菌间的侵入能力差异无统计学意义(P＞0.05).结论 4种常见牙周致病菌Pg33277、Pi25611、Aa29522和Fn10953均可黏附和侵入HUVEC,Pg33277侵入能力最强,这可能为其进一步发挥相关的生物学作用奠定基础.
目的 研究和比較4種常見牙週緻病菌Pg33277、Pi25611、Aa29522和Fn10953黏附和侵入人臍靜脈內皮細胞(human umbilical vein endothelial cells,HUVEC)的能力,為深入探討牙週緻病菌感染HUVEC在促進動脈粥樣硬化髮生、髮展中的作用奠定初步基礎.方法 建立牙週緻病菌感染HUVEC的體外模型,採用掃描電鏡、抗生素保護-菌落計數法觀察以上牙週緻病菌黏附和侵入HUVEC的能力.結果掃描電鏡結果錶明Pg33277、Pi25611、Aa29522和Fn10953均可黏附于HUVEC;抗生素保護法結果髮現Pg33277、Pi25611、Aa29522和Fn10953均可侵入HUVEC,細菌侵入量分彆為(0.8±0.1)×10~8、(4.1±0.5)×10~6、(1.6±0.3)×10~6、(5.0±0.4)×10~6CFU/L,侵入率分彆為(0.400±0.050)%、(0.021±0.003)%、(0.008±0.002)%和(0.025±0.002)%,Pg33277侵入能力遠較其他3種牙週緻病菌彊(P＜0.001),其餘3種牙週緻病菌間的侵入能力差異無統計學意義(P＞0.05).結論 4種常見牙週緻病菌Pg33277、Pi25611、Aa29522和Fn10953均可黏附和侵入HUVEC,Pg33277侵入能力最彊,這可能為其進一步髮揮相關的生物學作用奠定基礎.
목적 연구화비교4충상견아주치병균Pg33277、Pi25611、Aa29522화Fn10953점부화침입인제정맥내피세포(human umbilical vein endothelial cells,HUVEC)적능력,위심입탐토아주치병균감염HUVEC재촉진동맥죽양경화발생、발전중적작용전정초보기출.방법 건립아주치병균감염HUVEC적체외모형,채용소묘전경、항생소보호-균락계수법관찰이상아주치병균점부화침입HUVEC적능력.결과소묘전경결과표명Pg33277、Pi25611、Aa29522화Fn10953균가점부우HUVEC;항생소보호법결과발현Pg33277、Pi25611、Aa29522화Fn10953균가침입HUVEC,세균침입량분별위(0.8±0.1)×10~8、(4.1±0.5)×10~6、(1.6±0.3)×10~6、(5.0±0.4)×10~6CFU/L,침입솔분별위(0.400±0.050)%、(0.021±0.003)%、(0.008±0.002)%화(0.025±0.002)%,Pg33277침입능력원교기타3충아주치병균강(P＜0.001),기여3충아주치병균간적침입능력차이무통계학의의(P＞0.05).결론 4충상견아주치병균Pg33277、Pi25611、Aa29522화Fn10953균가점부화침입HUVEC,Pg33277침입능력최강,저가능위기진일보발휘상관적생물학작용전정기출.
Objective To investigate the adhesive and invasive ability of four common periodontal pathogens,Pg33277,Pi25611,Aa29522 and Fn10953 in human umbilical vein endothelial cells(HUVEC).Methods The model of infection of HUVEC by periodontal pathogens was established in vitro.The invasive ability of four periodontal pathogens in HUVEC was tested by scanning electron microscope(SEM)and antibiotic protection assays-colony-forming units(CFU).Results All of the four periodontal pathogens were found to adhere to HUVEC by SEM and invaded HUVEC at invasion numbers of(0.8±0.1)×10~8,(4.1±0.5)×10~6,(1.6±0.3)×10~6 and(5.0±0.4)×10~6 CFU/L respectively by antibiotic protection assays-CFU.The invasion efficiencies were(0.400±0.050)%,(0.021±0.003)%,(0.008±0.002)% and(0.025±0.002)%,respectively.The invasive ability of Pg33277 was significantly greater than those of the other three periodontal pathogens(P＜0.001).There was no difference in invasive abilities among Pi25611,Aa29522 and Fnl0953(P＞0.05).Conclusions All of the four common periodontal pathogens,Pg33277,Pi25611,Aa29522 and Fnl0953 could adhere to and invaded HUVEC,with Pg33277 being the strongest.
