CAJ | 학술논문

目的了解长春地区四种主要腹泻病毒病原构成及流行病学特点.方法收集长春市儿童医院5岁以下住院患儿腹泻样本共460例,轮状病毒采用ELISA试剂盒检测,杯状病毒、星状病毒采用逆转录-聚合酶链反应( RT-PCR)法,腺病毒采用聚合酶链反应(PCR)法进行鉴定.结果 460份标本中轮状病毒占35.22％( 162/460);杯状病毒占20.43％( 94/460),星状病毒占9.78％(45/460),腺病毒占3.70％(17/460),混合感染达7.17％(33/460),发病患儿以2岁以下婴幼儿为主.对162份轮状病毒阳性标本进行G/P分型,结果示G1P[8]为主要流行株,杯状病毒以GⅡ-4亚型为主要流行株,星状病毒为Ⅰ型,腺病毒为Ad41.结论长春地区婴幼儿病毒性腹泻的病原中轮状病毒是最主要病原,其次为杯状病毒、星状病毒和腺病毒.
목적 료해장춘지구사충주요복사병독병원구성급류행병학특점.방법 수집장춘시인동의원5세이하주원환인복사양본공460례,륜상병독채용ELISA시제합검측,배상병독、성상병독채용역전록-취합매련반응( RT-PCR)법,선병독채용취합매련반응(PCR)법진행감정.결과 460빈표본중륜상병독점35.22％( 162/460);배상병독점20.43％( 94/460),성상병독점9.78％(45/460),선병독점3.70％(17/460),혼합감염체7.17％(33/460),발병환인이2세이하영유인위주.대162빈륜상병독양성표본진행G/P분형,결과시G1P[8]위주요류행주,배상병독이GⅡ-4아형위주요류행주,성상병독위Ⅰ형,선병독위Ad41.결론 장춘지구영유인병독성복사적병원중륜상병독시최주요병원,기차위배상병독、성상병독화선병독.
Objective To study the pathogen and characteristics of viral diarrhea in children in Changchun area.Methods 460 stools specimens were collected from children with acute diarrhea cured in the childrens,hospital of Changchun in 2010.Rotavirus were detected by ELISA,caliceverus and astrovirus were detected by reverse transcription-polymerase chain reactions (RT-PCR),adenovirus were detected by polymerase chain reactions (PCR).Results A total of 460 specimens were detected.The detection rate of rotavirus,caliceverus,astrovious,adenovious respectively is 35.22％,20.43％,9.78％, 3.70％,the detectablerate of mixed infection is 7.61％,children under 2 years old were the major patient.The main genotypes of the virus:rotavirus ( G3P [ 8 ] ),caliceverus ( GⅡ-4 ),astrovious ( typeⅠ ),adenovious ( Ad41 ).Conclusion Rotavirus is the main pathogen in Changchun.Followed by caliceverus,astrovious,adenovious.