CAJ | 학술논문

目的探讨腺苷A_(2A)受体基因敲除小鼠在瘢痕形成中的作用及其机制.方法 4周大腺苷A_(2A)受体基因敲除小鼠和同窝野生型小鼠各12只,制作瘢痕模型,利用HE染色观察瘢痕组织厚度、横截面积变化情况.采用比色氯胺T法测量组织羟脯氨酸含量,利用Western免疫印迹测量TGF-β表达.结果野生型组小鼠瘢痕增生明显,其厚度、横截面积比自身对照增加倍数显著大于腺苷A_(2A)受体基因敲除组小鼠(P<0.01),腺苷A_(2A)受体基因敲除小鼠瘢痕增生轻,羟脯氨酸含量与同窝野生型组小鼠瘢痕含量相比差异有统计学(P<0.01),野生型组小鼠瘢痕TGF-β表达比自身对照增加倍数显著大于腺苷A_(2A)受体基因敲除小鼠组(P<0.01).结论腺苷A_(2A)受体基因敲除小鼠瘢痕TGF-β表达降低,瘢痕增生显著减轻.
목적 탐토선감A_(2A)수체기인고제소서재반흔형성중적작용급기궤제.방법 4주대선감A_(2A)수체기인고제소서화동와야생형소서각12지,제작반흔모형,이용HE염색관찰반흔조직후도、횡절면적변화정황.채용비색록알T법측량조직간포안산함량,이용Western면역인적측량TGF-β표체.결과 야생형조소서반흔증생명현,기후도、횡절면적비자신대조증가배수현저대우선감A_(2A)수체기인고제조소서(P<0.01),선감A_(2A)수체기인고제소서반흔증생경,간포안산함량여동와야생형조소서반흔함량상비차이유통계학(P<0.01),야생형조소서반흔TGF-β표체비자신대조증가배수현저대우선감A_(2A)수체기인고제소서조(P<0.01).결론 선감A_(2A)수체기인고제소서반흔TGF-β표체강저,반흔증생현저감경.
Objective To discuss the mechanism of scar hypertrophy in adenosine receptor A_(2A) (A_(2A) R) knockout mice. Methods Animal models of hypertrophic scar were established in 12 A_(2A)R knockout mice and 12 wild-type mice as control. The thickness and the size of transverse section of the hypertrophic scar were observed by H-E staining. The hydroxyproline ( HYP) in the scar was measured colorimetrically. The TGF-p expression was tested by Western blotting method. Results The hypertrophic scar in wild-type mice was more severe than that in knockout mice. Compared with self-control , the increase of the thickness and the size of transverse section of hypertrophic scar was markedly higher in wild-type group than in the knockout group( P < 0. 01) . There was significant difference in HYP content between the two groups (P < 0. 01 ). Compared with self-control, the increase of TGF-p expression in wild-type group was much more than that in knockout group (P < 0. 01 ). Conclusions The TGF-p expression decreases in the A_(2A) R knockout mice. The scar hypertrophy is also much less in the A_(2A) R knockout mice.