CAJ | 학술논문

目的本实验通过维甲酸对乳腺癌细胞的钠碘转运体(NIS)基因及蛋白表达和摄碘功能的影响,探讨乳腺癌细胞NIS的表达特点和调控因素.方法我们采用乳腺癌细胞MDA-MB453进行培养,分别给予不同浓度的维甲酸(RA)进行刺激72 h.采用RT-PCR方法检测细胞中NIS的mRNA表达;用WesternBloot方法检测NIS蛋白水平;采用125I测定乳腺癌细胞摄碘功能的变化.结果乳腺癌细胞MDA-MB453在经过RA刺激后,细胞中NIS的mRNA表达比对照组有明显增加,并且与RA浓度成正比.细胞中NIS的蛋白表达比对照组也有明显增加,并且与RA浓度相关.经过RA刺激的乳腺癌细胞摄碘能力明显增加.结论维甲酸对乳腺癌细胞MDA-MB453的NIS基因和蛋白表达均有促进作用,而且促进乳腺癌细胞的摄碘,这提示维甲酸可能通过调节乳腺癌NIS基因和蛋白的表达,从而影响乳腺癌细胞对放射性碘治疗的敏感性.
목적본실험통과유갑산대유선암세포적납전전운체(NIS)기인급단백표체화섭전공능적영향,탐토유선암세포NIS적표체특점화조공인소.방법아문채용유선암세포MDA-MB453진행배양,분별급여불동농도적유갑산(RA)진행자격72 h.채용RT-PCR방법검측세포중NIS적mRNA표체;용WesternBloot방법검측NIS단백수평;채용125I측정유선암세포섭전공능적변화.결과유선암세포MDA-MB453재경과RA자격후,세포중NIS적mRNA표체비대조조유명현증가,병차여RA농도성정비.세포중NIS적단백표체비대조조야유명현증가,병차여RA농도상관.경과RA자격적유선암세포섭전능력명현증가.결론유갑산대유선암세포MDA-MB453적NIS기인화단백표체균유촉진작용,이차촉진유선암세포적섭전,저제시유갑산가능통과조절유선암NIS기인화단백적표체,종이영향유선암세포대방사성전치료적민감성.
Objective: To investigate the effect of retionic acid on gene and protein expression of sodium-iodide symporter (NIS) and on the activity of iodide uptake in breast cancer cell (MDA-MB453). Methods: The breast cancer cell MDA-MB453 was cultured with negative control culture or with different concentration of retinoic acid for 72 hours. NIS gene mRNA in breast cancer cells was determined by reverse transcriptase-polymerase chain reaction (RT-PCR). NIS protein was checked by Western Blot. And the activity of iodide uptake was also assessed. Results:Gene mRNA and protein of sodium iodide symporter could be found increasing along with the concentration of retinoic acid. And augment of the activity of iodide uptake had also been indetified. Conclusion: Retionoic acid may play a role in iodide-uptake modulating in breast cancer cells by the effect on NIS gene and protein expression.