CAJ | 학술논문

目的探讨川芎嗪联用左旋精氨酸对心肌缺血-再灌注损伤时心肌细胞线粒体功能的影响.方法选用日本大耳白兔50只,随机分为正常对照组(A组)、心肌缺血-再灌注组(B组)、心肌缺血-再灌注+川芎嗪治疗组(C组)、心肌缺血-再灌注+左旋精氨酸治疗组(D组)和心肌缺血-再灌注+川芎嗪+左旋精氨酸治疗组(E组).观察心肌线粒体呼吸功能、Ca2+浓度、丙二醛浓度、超氧化物歧化酶活性和心肌组织三磷酸腺苷(ATP)和能荷的变化.结果与A组比较,B组线粒体呼吸控制率、Ⅲ态呼吸速率和超氧化物歧化酶明显降低,Ⅳ态呼吸速率、Ca2+浓度和丙二醛显著升高,心肌组织ATP和能荷明显降低.与B组比较,C组、D组和E组线粒体呼吸控制率、Ⅲ态呼吸速率和超氧化物歧化酶明显升高,Ⅳ态呼吸速率、Ca2+浓度、丙二醛显著降低,心肌组织ATP和能荷明显增高;且与A组比较,E组上述指标均无明显差异.结论川芎嗪联用左旋精氨酸可通过降低氧自由基水平和减轻钙超载,而改善缺血-再灌注损伤心肌的线粒体功能.
목적 탐토천궁진련용좌선정안산대심기결혈-재관주손상시심기세포선립체공능적영향.방법 선용일본대이백토50지,수궤분위정상대조조(A조)、심기결혈-재관주조(B조)、심기결혈-재관주+천궁진치료조(C조)、심기결혈-재관주+좌선정안산치료조(D조)화심기결혈-재관주+천궁진+좌선정안산치료조(E조).관찰심기선립체호흡공능、Ca2+농도、병이철농도、초양화물기화매활성화심기조직삼린산선감(ATP)화능하적변화.결과 여A조비교,B조선립체호흡공제솔、Ⅲ태호흡속솔화초양화물기화매명현강저,Ⅳ태호흡속솔、Ca2+농도화병이철현저승고,심기조직ATP화능하명현강저.여B조비교,C조、D조화E조선립체호흡공제솔、Ⅲ태호흡속솔화초양화물기화매명현승고,Ⅳ태호흡속솔、Ca2+농도、병이철현저강저,심기조직ATP화능하명현증고;차여A조비교,E조상술지표균무명현차이.결론 천궁진련용좌선정안산가통과강저양자유기수평화감경개초재,이개선결혈-재관주손상심기적선립체공능.
Aim To study the effect of ligustrazini (LGT) and L-arginine (L-Arg) on function of mitochondria in myocardial cell during myocardial ischemia-reperfusion injury (MIRI).Methods 50 animals were randomly divided into five groups (n=10 in each): control group(A), MIR group (B), MIR+LGT group (C), MIR+L-Arg group (D), MIR+LGT+L-Arg group (E). The mitochondrial respiratory function,Ca2+ concentration([Ca2+]m), malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were determined. Meanwhile, the contents of ATP and EC in the myocardial tissue were measured, respectively.Results It was found that mitochondrial respiratory control rate (RCR),state 3 (ST3), SOD, ATP and EC levels of myocardial tissue in C, D, E group were higher than those of B group, but state 4 (ST4), [Ca2+]m, MDA were lower. However, between E and A group, all parameters had no significant differences.Conclusion LGT and L-Arg can improve function of mitochondria in myocardial cells in the reperfusion injury after myocardial ischemia by decreasing oxygen free radical level and Ca2+ overload in the mitochondria.