农业科学与技术（英文版）
農業科學與技術（英文版）
농업과학여기술（영문판）
AGRICULTURAL SCIENCE & TECHNOLOGY
2009年
4期
61-64,74
,共5页
向倩%周兰英%万静%张旭%雷宝盛%金银春%冯毅%于绪任%赵晓英
嚮倩%週蘭英%萬靜%張旭%雷寶盛%金銀春%馮毅%于緒任%趙曉英
향천%주란영%만정%장욱%뢰보성%금은춘%풍의%우서임%조효영
Jatropha curcas Linn%cpSSR-PCR%Optimization of reaction system
[Objective] The aim of this study was to establish the optimum cpSSR-PCR system for Jatropha curcas Linn. [Method] cpSSR-PCR amplification system for Jatropha curcas Linn influenced by five factors including Taq DNA polymerase, Mg2+, DNA template, dNTP and primer were optimized from several levels. [Result] The optimum concentration of 20 μl reaction system was 10×Buffer, 2.00 mmol/L Mg2+, 2 U/μl Taq DNA polymerase, 0.2 mmol/L dNTP, 0.2 μmol/L primer and 35 ng/μl DNA template. [Conclusion] The optimum annealing temperature for cpSSR-PCR reaction system is 52 ℃, and the cpSSR reaction system is steady and reproducible.
