基因组学与应用生物学
基因組學與應用生物學
기인조학여응용생물학
GENOMICS AND APPLIED BIOLOGY
2010年
1期
164-169
,共6页
巴西橡胶树%胶乳%磷脂酰肌醇转移蛋白%RACE%序列分析
巴西橡膠樹%膠乳%燐脂酰肌醇轉移蛋白%RACE%序列分析
파서상효수%효유%린지선기순전이단백%RACE%서렬분석
Hevea brasiliensis%Latex%Phosphatidylinositol transfer protein%RACE%Sequence analysis
本文从巴西橡胶树(Hevea brasiliensis)差减cDNA文库中筛选到一个与磷脂酰肌醇转移蛋白(phos-phatidylinositol transfer protein)同源性较高的基因片段,并根据该基因片段序列信息,设计特异性引物,采用cDNA末端快速扩增技术RACE(rapid amplification of cDNA ends)进行差异片段的5'和3'端的扩增,并获得长度为1 08 1 bp的全长cDNA克隆R291(GenBank登陆号:AY589690).序列分析表明,该基因包含702 bp的开放阅读框,编码234个氨基酸,推测其蛋白质的分子量为26.8 kD,等电点为6.51,有一个的跨膜螺旋区(氨基酸位点为83～103).R291基因含有一个脂质结合保守区(Sec14p-like lipid-binding domain),具有CRAL-TRIO脂质结合结构域,推测该基因是一个磷脂酰肌醇转移蛋白基因.该基因的克隆将为橡胶树磷脂酰肌醇代谢的研究奠定了基础,将有助于进一步了解磷脂酰肌醇代谢与胶乳再生之间的关系.
本文從巴西橡膠樹(Hevea brasiliensis)差減cDNA文庫中篩選到一箇與燐脂酰肌醇轉移蛋白(phos-phatidylinositol transfer protein)同源性較高的基因片段,併根據該基因片段序列信息,設計特異性引物,採用cDNA末耑快速擴增技術RACE(rapid amplification of cDNA ends)進行差異片段的5'和3'耑的擴增,併穫得長度為1 08 1 bp的全長cDNA剋隆R291(GenBank登陸號:AY589690).序列分析錶明,該基因包含702 bp的開放閱讀框,編碼234箇氨基痠,推測其蛋白質的分子量為26.8 kD,等電點為6.51,有一箇的跨膜螺鏇區(氨基痠位點為83～103).R291基因含有一箇脂質結閤保守區(Sec14p-like lipid-binding domain),具有CRAL-TRIO脂質結閤結構域,推測該基因是一箇燐脂酰肌醇轉移蛋白基因.該基因的剋隆將為橡膠樹燐脂酰肌醇代謝的研究奠定瞭基礎,將有助于進一步瞭解燐脂酰肌醇代謝與膠乳再生之間的關繫.
본문종파서상효수(Hevea brasiliensis)차감cDNA문고중사선도일개여린지선기순전이단백(phos-phatidylinositol transfer protein)동원성교고적기인편단,병근거해기인편단서렬신식,설계특이성인물,채용cDNA말단쾌속확증기술RACE(rapid amplification of cDNA ends)진행차이편단적5'화3'단적확증,병획득장도위1 08 1 bp적전장cDNA극륭R291(GenBank등륙호:AY589690).서렬분석표명,해기인포함702 bp적개방열독광,편마234개안기산,추측기단백질적분자량위26.8 kD,등전점위6.51,유일개적과막라선구(안기산위점위83～103).R291기인함유일개지질결합보수구(Sec14p-like lipid-binding domain),구유CRAL-TRIO지질결합결구역,추측해기인시일개린지선기순전이단백기인.해기인적극륭장위상효수린지선기순대사적연구전정료기출,장유조우진일보료해린지선기순대사여효유재생지간적관계.
In this paper, a cDNA clone was isolated related to lipid transportation and metabolism by screening of a subtracted latex cDNA library of Hevea brasiliensis. According to its sequences information, a novel full-length cDNA termed R291 (GenBank number: AY589690) was obtained by using rapid amplification of cDNA ends (RACE). R291 was 1 081 bp long containing a 702 bp ORF, encoding 234 amino acids with a theoretical molecu lar weight of 26.8 kD and an isoeleetric point of 6.51. In addition, hydropathy and transmembrane motif analysis of de-duced amino acid sequences indicated that R29I possessed a transmembrane spanning domain (from the amino acid site from 83 to 103). The result of the conserved domains analysis demonstrated that R291 had a Sec14p-like lipid-binding domain and a CRAL/TRIO region. Multialignment presumed that R291 might be a phosphatidylinos-itol transfer protein gene. The cloning of this gene would establish the basic for metabolisim ofHevea brasiliensis phosphatidylinositol, and would help to further understand relation between metabolisim of phosphatidylinositol and latex regeneration.
