CAJ | 학술논문

目的:研究棕叶茎叶乙醇提取物对己烯雌酚预处理后大鼠、小鼠、豚鼠、家兔离体子宫及家兔在体子宫平滑肌的兴奋作用.方法:采用常规方法制备动物离体子宫标本,运用BL-420生物信号系统记录离体与在体子宫平滑肌的活动,以子宫收缩的幅度、频率及收缩力作为指标,观察棕叶茎叶乙醇提取物对注射己烯雌酚后大鼠、小鼠、豚鼠、家兔离体子宫及家兔在体子宫平滑肌的影响,计算兴奋子宫的IC_(50),以缩宫素为阳性对照.结果:棕叶茎叶95%乙醇和50%乙醇提取物对大鼠、小鼠、豚鼠、家兔离体子宫平滑肌有明显地兴奋作用,可加快收缩频率、提高收缩张力(P<0.05,P<0.01),并呈量效关系.50%乙醇提取物对家兔在体子宫平滑肌也有显著必奋作用(P<0.01).结论:棕叶茎叶乙醇提取物对啮齿目动物子宫的兴奋作用表明民间使用棕叶花治疗功能性子宫出血是有药理依据的.
목적:연구종협경협을순제취물대기희자분예처리후대서、소서、돈서、가토리체자궁급가토재체자궁평활기적흥강작용.방법:채용상규방법제비동물리체자궁표본,운용BL-420생물신호계통기록리체여재체자궁평활기적활동,이자궁수축적폭도、빈솔급수축력작위지표,관찰종협경협을순제취물대주사기희자분후대서、소서、돈서、가토리체자궁급가토재체자궁평활기적영향,계산흥강자궁적IC_(50),이축궁소위양성대조.결과:종협경협95%을순화50%을순제취물대대서、소서、돈서、가토리체자궁평활기유명현지흥강작용,가가쾌수축빈솔、제고수축장력(P<0.05,P<0.01),병정량효관계.50%을순제취물대가토재체자궁평활기야유현저필강작용(P<0.01).결론:종협경협을순제취물대교치목동물자궁적흥강작용표명민간사용종협화치료공능성자궁출혈시유약리의거적.
AIM: To study the exciting effects of ethanol extract of capitate phrynium (Phrynium Capitatum Willd) stem and leaf on the uterine smooth muscle of rat,mice,guinea pig and rabbit pretreated by diethylstilbestrol. METHODS: Conventional method was adopted to prepare animal uterine specimens both in vivo and in vitro. Different doses of the extract were given to the specimens and recorded their tensility, frequency and activity of contraction with the help of BL-420 biological experimental system. IC_(50) of the extract was calculated, with oxytocin as standard comparison. RESULTS: 95% ethanol extract and 50% ethanol extract of capitate phrynium and leaf had obviously stimulating action on the uterine smooth muscle of rat, mice, guinea pig and rabbit in a dose-dependent manner in vitro, which include accelerating contraction frequency, raising tension (P<0.05, P<0.01). In addition, 50% ethanol extract also had a significant stimulating action on uterine smooth muscle of rabbit in vivo(P< 0.01). CONCLUSION: Ethanol extracts of capitate phrynium have an effect on rodent uterine. Smooth muscle indicates that clinical application of Chinese practitioner used to treat the dysfunctional uterine bleeding proves to be effective.