海洋学报(中文版)
海洋學報(中文版)
해양학보(중문판)
ACTA OCEANOLOGICA SINICA
2010年
1期
130-138
,共9页
吕振明%李焕%吴常文%樊甄姣%张建设
呂振明%李煥%吳常文%樊甄姣%張建設
려진명%리환%오상문%번견교%장건설
地理群体%短蛸%遗传变异
地理群體%短蛸%遺傳變異
지리군체%단소%유전변이
geographycal population%Octopus ocellatus%genetic variation
采用线粒体COI基因测序技术分析了我国沿海重要经济头足类短蛸(Octopus ocellatus)6个地理群体的遗传结构及其变异,结果表明我国短蛸资源的遗传多样性相对丰富,在654 bp长度的核苷酸片段中共检测到42个多态位点,多态位点比例达6.42%,检测的60个个体中共获23个单倍型,单倍型多样性指数为0.200~0.867,平均核苷酸多样性指数为0.000 3~0.009 7,平均核苷酸差异数为0.200~6.311,高于多数海洋头足类的遗传变异.对6个群体的遗传结构进行检测表明,我国的短蛸群体间除部分群体外均存在显著的遗传分化(P<0.05),遗传结构基本符合脚踏石模型.聚类分析表明6群体已明显分化为两个类群,一个由大连、青岛、连云港群体组成,另一个由舟山、上海和广州群体组成,两类群间存在20个固定位点核苷酸差异,编码的蛋白质存在2个固定氨基酸残基的差异.两类群的分化系数达0.860 2(P<0.01),基因流大大小于1;AMOVA检测显示仅有12.72%的遗传差异存在于群体内部,而87.28%的遗传差异存在于群体间.短蛸遗传变异的研究成果可为今后我国短蛸资源更好的开发和管理提供理论基础.
採用線粒體COI基因測序技術分析瞭我國沿海重要經濟頭足類短蛸(Octopus ocellatus)6箇地理群體的遺傳結構及其變異,結果錶明我國短蛸資源的遺傳多樣性相對豐富,在654 bp長度的覈苷痠片段中共檢測到42箇多態位點,多態位點比例達6.42%,檢測的60箇箇體中共穫23箇單倍型,單倍型多樣性指數為0.200~0.867,平均覈苷痠多樣性指數為0.000 3~0.009 7,平均覈苷痠差異數為0.200~6.311,高于多數海洋頭足類的遺傳變異.對6箇群體的遺傳結構進行檢測錶明,我國的短蛸群體間除部分群體外均存在顯著的遺傳分化(P<0.05),遺傳結構基本符閤腳踏石模型.聚類分析錶明6群體已明顯分化為兩箇類群,一箇由大連、青島、連雲港群體組成,另一箇由舟山、上海和廣州群體組成,兩類群間存在20箇固定位點覈苷痠差異,編碼的蛋白質存在2箇固定氨基痠殘基的差異.兩類群的分化繫數達0.860 2(P<0.01),基因流大大小于1;AMOVA檢測顯示僅有12.72%的遺傳差異存在于群體內部,而87.28%的遺傳差異存在于群體間.短蛸遺傳變異的研究成果可為今後我國短蛸資源更好的開髮和管理提供理論基礎.
채용선립체COI기인측서기술분석료아국연해중요경제두족류단소(Octopus ocellatus)6개지리군체적유전결구급기변이,결과표명아국단소자원적유전다양성상대봉부,재654 bp장도적핵감산편단중공검측도42개다태위점,다태위점비례체6.42%,검측적60개개체중공획23개단배형,단배형다양성지수위0.200~0.867,평균핵감산다양성지수위0.000 3~0.009 7,평균핵감산차이수위0.200~6.311,고우다수해양두족류적유전변이.대6개군체적유전결구진행검측표명,아국적단소군체간제부분군체외균존재현저적유전분화(P<0.05),유전결구기본부합각답석모형.취류분석표명6군체이명현분화위량개류군,일개유대련、청도、련운항군체조성,령일개유주산、상해화엄주군체조성,량류군간존재20개고정위점핵감산차이,편마적단백질존재2개고정안기산잔기적차이.량류군적분화계수체0.860 2(P<0.01),기인류대대소우1;AMOVA검측현시부유12.72%적유전차이존재우군체내부,이87.28%적유전차이존재우군체간.단소유전변이적연구성과가위금후아국단소자원경호적개발화관리제공이론기출.
Studies on genetic variation in marine organisms are an important component of successful and sustainable management of marine fishery resources.Genetic variation of six Octopus ocellatus populations in China's coastal waters were investigated using the mitochondrial gene(COI) sequencing method.Results show that,compared with other cephalopod species,O.ocellatus shows higher genetic diversity in populations.42 polymorphic sites were detected in 654 bp aligned sequence,which reprented for 6.42% of the total length of sequenced COI gene.23 hapotypes were detected out of 60 individuals from six populations.The hapotype diversity,mean nucleotide diversity and average number of nucleotide differences reached 0.200~0.867,0.000 3 ~ 0.009 7,0.200~6.311,respectively.Molecular variance analysis revealed strong genetc sturcture in six O.ocellatus populations(P<0.05).A pattern of genetic-isolation-by-distance,comforming to a stepping stone model,was detected when plotting gene flow against geographic distance.UPGMA tree constructed shows that six O.ocellatus populations studied can be divided into two lineages.One is consisited of Dalian,Qingdao,Lianyungang plulations.The other is consisted of other three populations.Between them,20 fixed nucleotide sites and two fixed amino acid sites subtitute were detected along the gene sequence and coded protein,respectively.The Fst between them reached 0.8602 (P<0.01) and the gene flow between them turned out to be much lower than 1.AMOVA test,which showing 12.72% of the genetic variation lies within populations and 87.28% lies between populations,further suggested the genetic differentiation between these two lineages.These findings in the aspect of genetic variation of O.ocellatus will contribute to better exploitment and refined management of this precious cephalopod resources along the coast of China in future.