中山大学学报（医学科学版）
中山大學學報（醫學科學版）
중산대학학보（의학과학판）
JOURNAL OF SUN YAT-SEN UNIVERSITY(MEDICAL SCIENCES)
2010年
2期
207-212
,共6页
田疆%周经姣%陈艺韵%梁瑜%晏辉钧%周俊梅%刘岩%付春云%高洪丽%方丹云%狄飚%江丽芳
田疆%週經姣%陳藝韻%樑瑜%晏輝鈞%週俊梅%劉巖%付春雲%高洪麗%方丹雲%狄飚%江麗芳
전강%주경교%진예운%량유%안휘균%주준매%류암%부춘운%고홍려%방단운%적표%강려방
甲型H1N1流感病毒%神经氨酸酶基因%遗传进化
甲型H1N1流感病毒%神經氨痠酶基因%遺傳進化
갑형H1N1류감병독%신경안산매기인%유전진화
influenza A/H1N1 virus%neuraminidase gene%genetic evolution
[目的]了解季节性H1N1流感病毒与2009年新型H1N1流感病毒神经氨酸酶(NA)基因的遗传进化关系,探讨甲型H1N1流感病毒的遗传变异规律.[方法]分别从2006年和2009年流感病人标本中分离并鉴定出季节性H1N1流感病毒和新型H1N1流感病毒,用RT-PCR技术扩增了病毒NA基因全序列,并对其分子进化和重要功能位点的遗传变异进行了分析.[结果]2009年新型H1N1流感病毒与2006年季节性H1N1流感病毒比较,NA基因的同源性较低(77.9%～78.8%),与世界各地不同年代代表株及WHO推荐的1979～2010年季节性流感疫苗株比较,NA基因的同源性也较低(78.1%～79.3%).但与WHO推荐的2009年新型H1N1流感疫苗株比较同源性则高达99%以上;系统进化分析结果表明,2009年新型H1N1流感病毒NA基因与欧亚猪流感病毒株A/swine/Belgium/1/1983的亲缘关系最近;并发现自2005年以来季节性H1N1流感病毒NA基因的某些抗原位点和神经氨酸酶活性位点已发生了变异.[结论]2009年新型H1N1流感病毒NA基因可能来源于欧亚猪流感病毒;接种季节性流感疫苗不能对本次流行的新型流感产生有效的免疫保护作用;季节性H1N1流感病毒在流行过程中NA基因已发生了一定的变异,有必要持续跟踪和监测病毒的变异情况.
[目的]瞭解季節性H1N1流感病毒與2009年新型H1N1流感病毒神經氨痠酶(NA)基因的遺傳進化關繫,探討甲型H1N1流感病毒的遺傳變異規律.[方法]分彆從2006年和2009年流感病人標本中分離併鑒定齣季節性H1N1流感病毒和新型H1N1流感病毒,用RT-PCR技術擴增瞭病毒NA基因全序列,併對其分子進化和重要功能位點的遺傳變異進行瞭分析.[結果]2009年新型H1N1流感病毒與2006年季節性H1N1流感病毒比較,NA基因的同源性較低(77.9%～78.8%),與世界各地不同年代代錶株及WHO推薦的1979～2010年季節性流感疫苗株比較,NA基因的同源性也較低(78.1%～79.3%).但與WHO推薦的2009年新型H1N1流感疫苗株比較同源性則高達99%以上;繫統進化分析結果錶明,2009年新型H1N1流感病毒NA基因與歐亞豬流感病毒株A/swine/Belgium/1/1983的親緣關繫最近;併髮現自2005年以來季節性H1N1流感病毒NA基因的某些抗原位點和神經氨痠酶活性位點已髮生瞭變異.[結論]2009年新型H1N1流感病毒NA基因可能來源于歐亞豬流感病毒;接種季節性流感疫苗不能對本次流行的新型流感產生有效的免疫保護作用;季節性H1N1流感病毒在流行過程中NA基因已髮生瞭一定的變異,有必要持續跟蹤和鑑測病毒的變異情況.
[목적]료해계절성H1N1류감병독여2009년신형H1N1류감병독신경안산매(NA)기인적유전진화관계,탐토갑형H1N1류감병독적유전변이규률.[방법]분별종2006년화2009년류감병인표본중분리병감정출계절성H1N1류감병독화신형H1N1류감병독,용RT-PCR기술확증료병독NA기인전서렬,병대기분자진화화중요공능위점적유전변이진행료분석.[결과]2009년신형H1N1류감병독여2006년계절성H1N1류감병독비교,NA기인적동원성교저(77.9%～78.8%),여세계각지불동년대대표주급WHO추천적1979～2010년계절성류감역묘주비교,NA기인적동원성야교저(78.1%～79.3%).단여WHO추천적2009년신형H1N1류감역묘주비교동원성칙고체99%이상;계통진화분석결과표명,2009년신형H1N1류감병독NA기인여구아저류감병독주A/swine/Belgium/1/1983적친연관계최근;병발현자2005년이래계절성H1N1류감병독NA기인적모사항원위점화신경안산매활성위점이발생료변이.[결론]2009년신형H1N1류감병독NA기인가능래원우구아저류감병독;접충계절성류감역묘불능대본차류행적신형류감산생유효적면역보호작용;계절성H1N1류감병독재류행과정중NA기인이발생료일정적변이,유필요지속근종화감측병독적변이정황.
[Objective]This study was designed to investigate the genetic evolution of the neuraminidase(NA)gene of seasonal A/H1N1 and 2009 novel A/H1N1 inflilenza virus,and discuss the genetic variation of influenza A virus.[Methods]The virus strains were separately isolated from the clinical samples collected in 2006 and 2009,and then identified as seasonal A/H1N1 and novel A/H1N1.The full length of the NA gene of these strains was amplified by RT-PCR.Then the genetic evolution and mutations of important functional sites were analyzed.[Results]The homology of NA gene between the 2009 novel A/H1N1 isolates and 2006 seasonal A/H1N1 isolates was low(77.9%～78.8%),so was the homology of NA gene between the 2009 novel A/H1N1 isolates and representative strains of different periods and 1979-2001 WHO recommended vaccine strains(78.1%～79.3%).But compared with the WHO recommended vaccine strains of 2009 novel A/H1N1,the homology reached more than 99%.The genetic evolution analysis revealed that NA gene of 2009 novel A/H1N1 had the closest genetic relationship with the swine influenza A virus(A/swine/Belgium/1/1983)from Eurasian Iineage,and some of the antigenic sites and neuraminidase active sites of NA gene of seasonal A/H1N1 were mutated after 2005.[Conclusion]The NA gene of 2009 novel A/H1N1 may originate from Eurasian Iineage of swine influenza virus.The variation of NA gene of seasonal A/H1N1 has occurred in a certain degree.Hence,it is very necessary to continuously monitor the variant of influenza A virus.
