CAJ | 학술논문

鉴定了一种新发生的大豆茎枯病病原菌.在酸化PDA培养基上所有病原菌分离物菌落呈白色,气生菌丝呈密集的卷毛状,有时部分区域显黄绿色;培养基背面开始无色,随后产生大的、扩展的黑色子座.在接种的大豆茎秆上产生大小不等的黑色子座和分散的分生孢子器.共产生2类分生孢子,其中α型分生孢子丰富,无色,单孢,椭圆至纺锤状形,大小4.05～7.57 μm×1.48～3.25 μm,含2个油滴;β型分生孢子极少见,无色,线形.在培养基及大豆茎秆上未发现有性态.致病性测定表明选择的分离物引起合丰25大豆100%植株发病和种子腐烂.用Alu I、Rsa I 和Hha I酶切通用引物对ITS4/ITS5扩增的rDNA-ITS产物,所有分离物都产生与大豆拟茎点种腐病菌Phomopsis longicolla一致的酶切谱带;序列比对发现测序的2个分离物ITS序列与GenBank中9个P.longicolla分离物ITS序列100%同源.这是我国首次报道大豆田间发现P.longicolla,而且能引发严重的大豆茎枯病.
감정료일충신발생적대두경고병병원균.재산화PDA배양기상소유병원균분리물균락정백색,기생균사정밀집적권모상,유시부분구역현황록색;배양기배면개시무색,수후산생대적、확전적흑색자좌.재접충적대두경간상산생대소불등적흑색자좌화분산적분생포자기.공산생2류분생포자,기중α형분생포자봉부,무색,단포,타원지방추상형,대소4.05～7.57 μm×1.48～3.25 μm,함2개유적;β형분생포자겁소견,무색,선형.재배양기급대두경간상미발현유성태.치병성측정표명선택적분리물인기합봉25대두100%식주발병화충자부란.용Alu I、Rsa I 화Hha I매절통용인물대ITS4/ITS5확증적rDNA-ITS산물,소유분리물도산생여대두의경점충부병균Phomopsis longicolla일치적매절보대;서렬비대발현측서적2개분리물ITS서렬여GenBank중9개P.longicolla분리물ITS서렬100%동원.저시아국수차보도대두전간발현P.longicolla,이차능인발엄중적대두경고병.
The pathogen causing soybean stem blight disease was newly identified.The isolate produced floccose dense white colonies with occasional green-yellowish areas on acidified PDA medium was newly identified.The stromata were large,black and widespread.It was observed that the pycnidia produced both alpha- and beta-conidia when inoculated on soybean stem.Alpha-conidia were hyaline,ellipsoidal to fusiform,and guttulate with sizes of 4.05 to 7.57μm ×1.48to 3.25μm.Beta-conidia were rare,hyaline,filiform and hamate.Four isolates selected caused seeds rotten and 12-day-old seedlings diseased when inoculated on soybean cultivar Hefeng 25.To indentified the pathogen,rDNA-ITS fragment was amplified using universal primer ITS4 and ITS5,and followed by digesting with Alu I,Rsa I and Hha I restriction enzymes. Phomopsis longicolla characteristic band spectrum was shown in every isolate PCR products.Two isolated ITS sequences were identical to 9 isolates of P.longicolla in GenBank.The results indicated that the pathogen of severe soybean stem blight was just P.longicolla.It was the first report of P.longicolla being isolated from soybean in China.