CAJ | 학술논문

[目的]研究绿菇（Russula virescens（Schaeff．）Fr．）对灰霉菌的拮抗作用。[方法]采用对峙培养测定法研究了绿菇菌丝体，发酵液及菌丝体提取液对灰霉菌的拈抗效果。[结果]绿菇对灰霉菌的拈抗作用主要表现为菌丝体重寄生和代谢产物生长抑制作用，对峙生长抑制率介于46．9％～52．3％，重寄生现象明显。PDB培养基培养绿菇获得的发酵液生长抑制作用最强，对辣椒灰霉菌生长抑制率最高，为61．59％；发酵液热稳定性良好；绿菇菌丝提取液对供试灰霉菌无生长抑制作用。[结论]该研究为灰霉菌的分化和药敏感性研究提供了理论依据。
[목적]연구록고（Russula virescens（Schaeff．）Fr．）대회매균적길항작용。[방법]채용대치배양측정법연구료록고균사체，발효액급균사체제취액대회매균적념항효과。[결과]록고대회매균적념항작용주요표현위균사체중기생화대사산물생장억제작용，대치생장억제솔개우46．9％～52．3％，중기생현상명현。PDB배양기배양록고획득적발효액생장억제작용최강，대랄초회매균생장억제솔최고，위61．59％；발효액열은정성량호；록고균사제취액대공시회매균무생장억제작용。[결론]해연구위회매균적분화화약민감성연구제공료이론의거。
[Objective] The paper was to study the antagonistic effect of Russula virescens （Schaeff.） Ft. against Bortrytis cinerea. [Method] Using the confrontation culture method, the antagonistic effect of mycelium, fermentation broth and mycelial extract of R. virescens against B. cinerea was studied. [Result] The antagonistic ef- fect of R. vivesscens against B. cinerea was mainly manifested as hyperparasitism of mycelium and inhibition effect of metabolites. The inhibition rate of confrontation growth was between 46.9% and 52.3%, and hyperparasitism phenomenon was obvi- ous. The fermentation broth of R. virescens cultured by PDB medium had the strongest growth inhibition effect, and the growth inhibition rate against B. cinerea was the highest of 61.59%. The thermal stability of fermentation broth was good, the mycelial extract of R. virescens had no inhibition effect against the growth of B. cinerea. [Conclusion] The study provided theoretical basis for subsequent differentiation and drug sensitivity of B. cinerea.