中国危重病急救医学
中國危重病急救醫學
중국위중병급구의학
CHINESE CRITICAL CARE MEDICINE
2010年
8期
473-476
,共4页
陶炳东%张锦%佟冬仪%魏会霞%于泓波
陶炳東%張錦%佟鼕儀%魏會霞%于泓波
도병동%장금%동동의%위회하%우홍파
内毒素休克%肺动脉%多巴胺%血管反应性
內毒素休剋%肺動脈%多巴胺%血管反應性
내독소휴극%폐동맥%다파알%혈관반응성
Endotoxic shock%Pulmonary artery%Dopamine%Vascular reactivity
目的 比较不同浓度多巴胺对大肠杆菌内毒素脂多糖(LPS)孵育的离体兔肺动脉、体动脉血管张力的影响.方法 选择6只雄性大耳白兔,制备离体肺动脉环和体动脉环各36个.把36个肺动脉环随机分为6组,测试不同浓度多巴胺(4×10-5、8×10-5、16×10-5 μmol/L)对正常肺动脉张力的影响(分别为PN-DOPA4、PN-DOPA8、PN-DOPA16组)及对LPS孵育后肺动脉张力的影响(分别为PL-DOPA4、PL-DOPA8、PL-DOPA16组).体动脉分组与肺动脉分组方法相同,包括正常组(SN-DOPA4、SN-DOPA8、SN-DOPA16)及内毒素组(SL-DOPA4、SL-DOPA8、SL-DOPA16).结果 ①多巴胺对PN-DOPA4及SN-DOPA4组中的血管环均有一定的舒张作用;对PN-DOPA8、PN-DOPA16、SN-DOPA8和SN-DOPA16组的血管环均有收缩作用,随着浓度的加大而升高.②LPS孵育后,多巴胺对PL-DOPA4和SL-DOPA4组血管环舒张作用消失,变为收缩[(22.60±6.68)%比-(2.25±0.58)%,(3.80±0.52)%比-(3.65±0.75)%,P<0.05和P<0.01],对PL-DOPA8组收缩幅度较PN-DOPA8组减少(14.52±0.59)%(P<0.05);对SL-DOPA8组收缩幅度较SN-DOPA8组增高(25.90±1.75)%(P<0.05);对PL-DOPA16组和SL-DOPA16组的张力无显著影响.③LPS孵育后,DOPA4组的肺动脉张力变化(PL/PN)较体动脉张力变化(SL/SN)明显(-10.90±5.06比-1.00±0.24,P<0.05);在DOPA8组SL/SN较明显(1.80±0.35比0.48±0.17,P<0.01).结论 低浓度的多巴胺对正常肺动脉及体动脉血管环有舒张作用;在LPS孵育后,低浓度的多巴胺对肺动脉及体动脉环的作用由舒张变为收缩,且对肺动脉环的张力变化影响最大;不同浓度的多巴胺对LPS孵育后的肺动脉及体动脉血管环均有收缩作用.
目的 比較不同濃度多巴胺對大腸桿菌內毒素脂多糖(LPS)孵育的離體兔肺動脈、體動脈血管張力的影響.方法 選擇6隻雄性大耳白兔,製備離體肺動脈環和體動脈環各36箇.把36箇肺動脈環隨機分為6組,測試不同濃度多巴胺(4×10-5、8×10-5、16×10-5 μmol/L)對正常肺動脈張力的影響(分彆為PN-DOPA4、PN-DOPA8、PN-DOPA16組)及對LPS孵育後肺動脈張力的影響(分彆為PL-DOPA4、PL-DOPA8、PL-DOPA16組).體動脈分組與肺動脈分組方法相同,包括正常組(SN-DOPA4、SN-DOPA8、SN-DOPA16)及內毒素組(SL-DOPA4、SL-DOPA8、SL-DOPA16).結果 ①多巴胺對PN-DOPA4及SN-DOPA4組中的血管環均有一定的舒張作用;對PN-DOPA8、PN-DOPA16、SN-DOPA8和SN-DOPA16組的血管環均有收縮作用,隨著濃度的加大而升高.②LPS孵育後,多巴胺對PL-DOPA4和SL-DOPA4組血管環舒張作用消失,變為收縮[(22.60±6.68)%比-(2.25±0.58)%,(3.80±0.52)%比-(3.65±0.75)%,P<0.05和P<0.01],對PL-DOPA8組收縮幅度較PN-DOPA8組減少(14.52±0.59)%(P<0.05);對SL-DOPA8組收縮幅度較SN-DOPA8組增高(25.90±1.75)%(P<0.05);對PL-DOPA16組和SL-DOPA16組的張力無顯著影響.③LPS孵育後,DOPA4組的肺動脈張力變化(PL/PN)較體動脈張力變化(SL/SN)明顯(-10.90±5.06比-1.00±0.24,P<0.05);在DOPA8組SL/SN較明顯(1.80±0.35比0.48±0.17,P<0.01).結論 低濃度的多巴胺對正常肺動脈及體動脈血管環有舒張作用;在LPS孵育後,低濃度的多巴胺對肺動脈及體動脈環的作用由舒張變為收縮,且對肺動脈環的張力變化影響最大;不同濃度的多巴胺對LPS孵育後的肺動脈及體動脈血管環均有收縮作用.
목적 비교불동농도다파알대대장간균내독소지다당(LPS)부육적리체토폐동맥、체동맥혈관장력적영향.방법 선택6지웅성대이백토,제비리체폐동맥배화체동맥배각36개.파36개폐동맥배수궤분위6조,측시불동농도다파알(4×10-5、8×10-5、16×10-5 μmol/L)대정상폐동맥장력적영향(분별위PN-DOPA4、PN-DOPA8、PN-DOPA16조)급대LPS부육후폐동맥장력적영향(분별위PL-DOPA4、PL-DOPA8、PL-DOPA16조).체동맥분조여폐동맥분조방법상동,포괄정상조(SN-DOPA4、SN-DOPA8、SN-DOPA16)급내독소조(SL-DOPA4、SL-DOPA8、SL-DOPA16).결과 ①다파알대PN-DOPA4급SN-DOPA4조중적혈관배균유일정적서장작용;대PN-DOPA8、PN-DOPA16、SN-DOPA8화SN-DOPA16조적혈관배균유수축작용,수착농도적가대이승고.②LPS부육후,다파알대PL-DOPA4화SL-DOPA4조혈관배서장작용소실,변위수축[(22.60±6.68)%비-(2.25±0.58)%,(3.80±0.52)%비-(3.65±0.75)%,P<0.05화P<0.01],대PL-DOPA8조수축폭도교PN-DOPA8조감소(14.52±0.59)%(P<0.05);대SL-DOPA8조수축폭도교SN-DOPA8조증고(25.90±1.75)%(P<0.05);대PL-DOPA16조화SL-DOPA16조적장력무현저영향.③LPS부육후,DOPA4조적폐동맥장력변화(PL/PN)교체동맥장력변화(SL/SN)명현(-10.90±5.06비-1.00±0.24,P<0.05);재DOPA8조SL/SN교명현(1.80±0.35비0.48±0.17,P<0.01).결론 저농도적다파알대정상폐동맥급체동맥혈관배유서장작용;재LPS부육후,저농도적다파알대폐동맥급체동맥배적작용유서장변위수축,차대폐동맥배적장력변화영향최대;불동농도적다파알대LPS부육후적폐동맥급체동맥혈관배균유수축작용.
Objective To compare the vasoactive effects of dopamine (DOPA) of different concentrations on isolated rabbit pulmonary and systemic arteries after incubation with lipopolysaccharide (LPS). Methods Six white male rabbits were used. Thirty-six pulmonary arterial rings and 36 systemic arterial rings were prepared. The 36 pulmonary arterial rings were divided into six groups to determine the effect of different concentrations of DOPA (4×10-5,8×10-5,16×10-5 μmol/L) on the tension of the normal pulmonary artery (PN-DOPA4, PN-DOPA8, PN-DOPA16 groups, respectively), and the tension of the pulmonary artery rings after being incubated with LPS (PL-DOPA4, PL-DOPA8, PL-DOPA16 groups, respectively). The 36 systemic arterial rings were also divided into six groups as the pulmonary arterial rings, including normal groups (SN-DOPA4, SN-DOPA8, SN-DOPA16) and LPS groups (SL-DOPA4, SL-DOPA8, SL-DOPA16). Results ① DOPA relaxed the arterial rings in PN-DOPA4 and SN-DOPA4 groups, while it produced contraction in PN-DOPA8, PN-DOPA16, SN-DOPA8 and SN-DOPA16 groups, and the contraction was more marked with the increase in concentration of DOPA. ② After preincubation with LPS, the relaxation property of DOPA in PL-DOPA4 and SL-DOPA4 groups was observed to be reversed to contraction (22.60±6.68)% vs.-(2.25±0.58)%, (3.80±0.52)% vs. -(3.65±0.75)%, P<0.05 and P<0.01]; the contraction response of DOPA in PL-DOPA8 group decreased compared with PN-DOPA8 group by (14.52±0.59)% (P<0.05), while increased by (25.90±1.75)% in SL-DOPA8 group compared with SN-DOPA8 group (P<0.05), and no response was observed in PL-DOPA16 and SL-DOPA16 groups. ③After reincubation with LPS, changes in pulmonary arterial tension (PL/PN) in DOPA4 group were more obvious than those in systemic arterial tension (SL/SN, -10.90±5.06 vs. -1.00±0.24, P<0.05), while the SL/SN in DOPA8 group were more obvious (1.80±0.35 vs. 0.48±0.17, P<0.01).Conclusion DOPA in low concentrations had the function of relaxation on the pulmonary arterial and systemic arterial rings. After the arterial rings are preincubated with LPS, the relaxation response of DOPA of low concentrations is changed to be vaso-contraction, and the changes in pulmonary arterial rings are most marked. DOPA of different concentrations all produce contraction effect on LPS-preincubated arterial rings.
