CAJ | 학술논문

目的了解产肺炎克雷伯菌碳青霉烯酶-2型(KPC-2)肺炎克雷伯菌中16S rRNA甲基化酶基因的分布.方法收集37株产KPC-2肺炎克雷伯菌,使用琼脂稀释法测定其对阿米卡星、庆大霉素和奈替米星的最小抑菌浓度(MIC),PCR扩增6种16S rRNA甲基化酶基因:armA、rmtA、rmtB、rmtC、rmtD和npmA.结果产KPC-2肺炎克雷伯菌对阿米卡星、庆大霉素和奈替米星的耐药率均为97.3%(MIC50≥1024μg/mL),其中8株检出arms基因,25株检出rmtB基因,同时检出armA和rmtB基因的有4株,未检测到rmtA、rmtC、rmtD和npmA阳性菌株.16S rRNA甲基化酶基因总阳性率为78.4%(29/37).结论 16S rRNA甲基化酶基因armA和rmtB在产KPC-2肺炎克雷伯菌中广泛分布.
목적 료해산폐염극뢰백균탄청매희매-2형(KPC-2)폐염극뢰백균중16S rRNA갑기화매기인적분포.방법 수집37주산KPC-2폐염극뢰백균,사용경지희석법측정기대아미잡성、경대매소화내체미성적최소억균농도(MIC),PCR확증6충16S rRNA갑기화매기인:armA、rmtA、rmtB、rmtC、rmtD화npmA.결과 산KPC-2폐염극뢰백균대아미잡성、경대매소화내체미성적내약솔균위97.3%(MIC50≥1024μg/mL),기중8주검출arms기인,25주검출rmtB기인,동시검출armA화rmtB기인적유4주,미검측도rmtA、rmtC、rmtD화npmA양성균주.16S rRNA갑기화매기인총양성솔위78.4%(29/37).결론 16S rRNA갑기화매기인armA화rmtB재산KPC-2폐염극뢰백균중엄범분포.
Objective To investigate the distribution of 16S rRNA methylase genes in Klebsiella pneumoniae strains producing Klebsiella pneumoniae ealbapenenase type 2(KPC-2).Methods A total of 37 Klebsiella pneumoniae isolates producing KPC-2 were collected.The minimal inhibitory concentrations (MICs)of these strains to amikacin,gentamyein and netilmicin were determinated by agal dilution method.Six 16S rRNA methylase genes(armA,rmtA,rmtB,rmtC,rmtD and npmA)were detected by PCR.Results The resistant rates to amikacin,gentamycin and netilmicin were 97.3%(MIC50≥1024μg/mL).Among those resistant strains,8 were armr/A positive,25 were rmtB positive,4 were both armA and rmtB positive.and no other 16S rRNA methylase genes were found.The total positive rate of 16S rRNA methylase genes was 78.4%(29/37).Conclusion 16S rRNA methylase genes armA and rmtB ale prevalent in Klebsiella pneumoniae strains producing KPC-2.