CAJ | 학술논문

目的比较分析胆日同醇结石病人与正常人胆囊胆汁蛋白表达谱的差异,为阐明胆石成因提供实验依据.方法提取胆固醇结石病人和正常人胆囊胆汁总蛋白,构建相应的双向电泳蛋白质表达谱,应用lmageMaster图像分析软件获得差异蛋白点信息.结果胆固醇结石病人与正常人胆囊胆汁总蛋白浓度分别为(6.4824±0.3403)mg/ml和(2.3156±0.3064)mg/ml(P<0.01).两组组内蛋白质平均匹配点数分别为465±71和407±85,平均匹配率为71.70%和68.38%,组间269个蛋白质点相互匹配,匹配率为61.70%.胆固醇结石组蛋白表达谱显著差异点34个,16个表达上调,18个表达下调.结论初步建立了胆固醇结石病人和正常人胆囊胆汁蛋白表达谱.发现两者存在差异蛋白质点,为筛选胆石形成关键调控子提供基础.
목적 비교분석담일동순결석병인여정상인담낭담즙단백표체보적차이,위천명담석성인제공실험의거.방법 제취담고순결석병인화정상인담낭담즙총단백,구건상응적쌍향전영단백질표체보,응용lmageMaster도상분석연건획득차이단백점신식.결과 담고순결석병인여정상인담낭담즙총단백농도분별위(6.4824±0.3403)mg/ml화(2.3156±0.3064)mg/ml(P<0.01).량조조내단백질평균필배점수분별위465±71화407±85,평균필배솔위71.70%화68.38%,조간269개단백질점상호필배,필배솔위61.70%.담고순결석조단백표체보현저차이점34개,16개표체상조,18개표체하조.결론 초보건립료담고순결석병인화정상인담낭담즙단백표체보.발현량자존재차이단백질점,위사선담석형성관건조공자제공기출.
Objective To elucidate the mechanism of formation of cholesterol cholelithiasis, thisstudy is designed to establish two-dimensional polyacrylamide gel electrophoresis system for differenti-al analysis of the proteome of gallbladder bile from cholesterol cholelithiasis patients and control ones.Methods Total proteins of gallbladder bile were extracted from the subjects in two groups and the protein profiles were established by using the immobilized pH gradient two-dimensional electrophoresis respectively. These maps were analyzed by ImageMaster two-dimensional eleetrophoresis analysis software. Results The concentrations of bile proteins from cholesterol cholelithiasis patients and con-trol ones were (6.4824±0.3403)mg/ml and (2.3156±0.3064)mg/ml(P<0.01), respectively. The average matched protein spots were 465±71 and 407±85 in the two groups, respectively. The matched rates were 71.70% and 68.38% correspondingly. There were 269 matched spots in the two reference gels maps and the matched rate was 61.70%. A total of 34 differential protein spots were i-dentified, 16 expressed highly in cholesterol cholelithiasis patients but lowly in the control ones, while 18 expressed just in the opposite way. Conclusion The differential protein profiles of gallbladder bile between cholesterol cholelithiasis patients and controls have been established and several differential protein spots are found. The data may be a basis for further screening the key regulators of formation of cholesterol eholelithiasis.