CAJ | 학술논문

目的探讨大鼠γ射线照射后唾液腺组织NBS1基因和蛋白的表达,及其在唾液腺上皮细胞放射性损伤修复中的调控作用.方法将80只大鼠按随机数字表法均分为健康对照组和照射组,各40只.照射组大鼠以60Co γ射线分次照射,每次3 Gy,隔天1次,共5次,累积剂量为3、6、9、12和15 Gy.应用电镜观察唾液腺细胞超微结构变化.用免疫组织化学技术(IHC)和反转录聚合酶链技术(RT-PCR)分别检测照射后2～4h的腮腺、颌下腺NBSl mRNA和蛋白的表达情况.结果照射组腮腺组织萎缩和损伤程度重于颌下腺.腮腺组织吸收剂量为9.12和15 Gy时、颌下腺组织吸收剂量为9和12 Gy时,NBSl mRNA表达水平明显降低(t=7.10、17.93、20.86,P ＜0.05;t=3.13和7.53,P ＜0.05).照射组腮腺浆液细胞吸收剂量为9.12和15 Gy时、导管上皮细胞剂量为12和15 Gy时,差异有统计学意义(t=4.29、17.91、91.29,P＜0.05；t =3.09和5.62,P＜0.05).颌下腺浆液细胞在12 Gy时,NBS1蛋白表达明显减少(t=4.61和11.84,P ＜0.05).颌下腺导管上皮细胞及黏液细胞其NBSI蛋白表达在整个照射过程中未见明显变化.结论 γ射线照射后大鼠唾液腺组织NBS1 mRNA和蛋白水平均出现下降,推测NBS1可能参与唾液腺放射损伤和修复的过程.
목적 탐토대서γ사선조사후타액선조직NBS1기인화단백적표체,급기재타액선상피세포방사성손상수복중적조공작용.방법 장80지대서안수궤수자표법균분위건강대조조화조사조,각40지.조사조대서이60Co γ사선분차조사,매차3 Gy,격천1차,공5차,루적제량위3、6、9、12화15 Gy.응용전경관찰타액선세포초미결구변화.용면역조직화학기술(IHC)화반전록취합매련기술(RT-PCR)분별검측조사후2～4h적시선、합하선NBSl mRNA화단백적표체정황.결과 조사조시선조직위축화손상정도중우합하선.시선조직흡수제량위9.12화15 Gy시、합하선조직흡수제량위9화12 Gy시,NBSl mRNA표체수평명현강저(t=7.10、17.93、20.86,P ＜0.05;t=3.13화7.53,P ＜0.05).조사조시선장액세포흡수제량위9.12화15 Gy시、도관상피세포제량위12화15 Gy시,차이유통계학의의(t=4.29、17.91、91.29,P＜0.05；t =3.09화5.62,P＜0.05).합하선장액세포재12 Gy시,NBS1단백표체명현감소(t=4.61화11.84,P ＜0.05).합하선도관상피세포급점액세포기NBSI단백표체재정개조사과정중미견명현변화.결론 γ사선조사후대서타액선조직NBS1 mRNA화단백수평균출현하강,추측NBS1가능삼여타액선방사손상화수복적과정.
Objective To investigate the expressions of NBS1 mRNA and protein in the salivary gland of irradiated rats and explore the role of NBS1 in the repair of radiation injury of salivary gland epithelial cells.Methods Eighty rats were randomly divided into two groups for radiation and control (n =40 each).The rats were fractionally exposed to 3 Gy of 60Co γ-rays once in two days,leading to an accumulation dose of 3,6,9,12,15 Gy.The sham-irradiated controls were anesthetized in parallel but without irradiation.After 2-4 h of irradiation,the rats were sacrificed,IHC and RT-PCR were used to detect the expressions of NBS1 protein and mRNA in parotid and submandibular glands,and the ultra-structural changes in the glands were observed by a transmission electron microscopy.Results After irradiation,the salivary glands became atrophy and the parotid gland cells were damaged more serious than the submandibular gland cells.Compared with the controls,with the groups of dose,at 9,12,15 Gy in parotid gland (t =7.10,17.93,20.86,P ＜ 0.05),at 12,15 Gy in the submandibular gland (t =3.13,7.53,P ＜0.05),the expression of NBS1 mRNA was reduced.With the groups of dose at 9,12,15 Gy in paretid gland (t =4.29,17.91,91.29,P ＜ 0.05 ),the dose at 12,15 Gy in submandibular gland ( t =4.61,11.84,P＜0.05),the expression of NBS1 protein in serous cells,and the dose at 12,15 Gy in parotid gland ductal epithelial cell ( t =3.09,5.62,P ＜ 0.05) were reduced.But in the ductal epithelial cells as well as muoass cells in the submandibualr gland were steadily.Conclusions After irradiation,NBS1 at both protein and mRNA levels was dropped in the salivary gland of rats,which might contribute to the repair of radiation injury of salivary gland.