中华内科杂志
中華內科雜誌
중화내과잡지
CHINESE JOURNAL OF INTERNAL MEDICINE
2011年
4期
311-315
,共5页
赵杰%蒋益%雷媛%陈立平%易烽明%王昌高%邹开芳%夏冰
趙傑%蔣益%雷媛%陳立平%易烽明%王昌高%鄒開芳%夏冰
조걸%장익%뢰원%진립평%역봉명%왕창고%추개방%하빙
结肠炎,溃疡性%多态性%单核苷酸%MICA-129基因%可溶性MICA
結腸炎,潰瘍性%多態性%單覈苷痠%MICA-129基因%可溶性MICA
결장염,궤양성%다태성%단핵감산%MICA-129기인%가용성MICA
Colitis,ulcerative%Polymorphism,single nucleotide%Major histocompatibility complex class Ⅰ chain-related antigens A -129%Soluble major histocompatibility complex class Ⅰ chain related antigens A
目的 探讨人类主要组织相容性复合体I类相关基因A(MICA)-129位点多态性及血清可溶性MICA(sMICA)水平与溃疡性结肠炎(UC)的关系.方法 采用聚合酶链反应-直接测序分型(PCR-SBT)方法 检测256例UC患者和460例正常对照者MICA-129的基因多态性;随机选取80例UC患者和90例正常对照者,采用ELISA法测定并比较血清sMICA的浓度差异.结果 UC组的MICA-129的G等位基因和GG基因型频率明显高于正常对照组(76.8%比72.2%,P=0.060;55.9%比46.3%,P=0.016);UC组sMICA水平明显高于正常对照组[(576.47±279.02)ng/L比(182.17±73.11)ng/L,P＜0.001].此外,携带GG基因型的UC患者的sMICA水平明显高于(GA+AA)基因型携带者[(638.87±347.15)ng/L比(507.51±152.87)ng/L,P=0.035].结论 MICA129基因多态性及sMICA水平与UC明显相关,MICA-129基因可能在UC的发病机制中发挥作用.
目的 探討人類主要組織相容性複閤體I類相關基因A(MICA)-129位點多態性及血清可溶性MICA(sMICA)水平與潰瘍性結腸炎(UC)的關繫.方法 採用聚閤酶鏈反應-直接測序分型(PCR-SBT)方法 檢測256例UC患者和460例正常對照者MICA-129的基因多態性;隨機選取80例UC患者和90例正常對照者,採用ELISA法測定併比較血清sMICA的濃度差異.結果 UC組的MICA-129的G等位基因和GG基因型頻率明顯高于正常對照組(76.8%比72.2%,P=0.060;55.9%比46.3%,P=0.016);UC組sMICA水平明顯高于正常對照組[(576.47±279.02)ng/L比(182.17±73.11)ng/L,P＜0.001].此外,攜帶GG基因型的UC患者的sMICA水平明顯高于(GA+AA)基因型攜帶者[(638.87±347.15)ng/L比(507.51±152.87)ng/L,P=0.035].結論 MICA129基因多態性及sMICA水平與UC明顯相關,MICA-129基因可能在UC的髮病機製中髮揮作用.
목적 탐토인류주요조직상용성복합체I류상관기인A(MICA)-129위점다태성급혈청가용성MICA(sMICA)수평여궤양성결장염(UC)적관계.방법 채용취합매련반응-직접측서분형(PCR-SBT)방법 검측256례UC환자화460례정상대조자MICA-129적기인다태성;수궤선취80례UC환자화90례정상대조자,채용ELISA법측정병비교혈청sMICA적농도차이.결과 UC조적MICA-129적G등위기인화GG기인형빈솔명현고우정상대조조(76.8%비72.2%,P=0.060;55.9%비46.3%,P=0.016);UC조sMICA수평명현고우정상대조조[(576.47±279.02)ng/L비(182.17±73.11)ng/L,P＜0.001].차외,휴대GG기인형적UC환자적sMICA수평명현고우(GA+AA)기인형휴대자[(638.87±347.15)ng/L비(507.51±152.87)ng/L,P=0.035].결론 MICA129기인다태성급sMICA수평여UC명현상관,MICA-129기인가능재UC적발병궤제중발휘작용.
Objective To investigate the association of the major histocompatibility complex class Ⅰ chain-related antigens A (MICA)-129 gene polymorphism and soluble MICA (sMICA) levels with ulcerative colitis (UC) in Hubei Han nationality. Methods The genetic polymorphism of MICA-129 was examined using a polymerase chain reaction-sequence based test (PCR-SBT) in 256 UC patients and 460 healthy controls. From the above subjects, 80 patients and 90 healthy individuals were randomly selected for determining serum sMICA concentrations by ELISA. Results The frequencies of variant allele (G) and genotype (GG) in MICA-129 gene were significantly higher in the UC patients than in the controls(76. 8%vs 72. 2%, P =0. 060; 55.9% vs 46. 3% ,P =0. 016). Serum sMICA levels were significantly elevated in the patients compared to the controls[(576. 47 ±279. 02) ng/L vs( 182. 17 ±73. 11 ) ng/L,P ＜0. 001]. In addition, the sMICA levels were higher in the patients carrying MICA-129 GG genotypes than in those carrying ( GA + AA) genotypes [( 638. 87 ± 347. 15 ) ng/L vs ( 507. 51 ± 152. 87 ) ng/L, P = 0. 035].Conclusions The genetic polymorphism of MICA-129 and sMICA levels are correlated with the UC patients in Hubei Han nationality. Our findings demonstrate that MICA-129 gene may contribute to the pathogenesis of UC.
