中华创伤骨科杂志
中華創傷骨科雜誌
중화창상골과잡지
CHINESE JOURNAL OF ORTHOPAEDIC TRAUMA
2011年
10期
951-955
,共5页
王伟%李秀翠%张伟%程少文%林忠勤%宋世峰%李丹%毕龙%王军%李国臣%彭磊
王偉%李秀翠%張偉%程少文%林忠勤%宋世峰%李丹%畢龍%王軍%李國臣%彭磊
왕위%리수취%장위%정소문%림충근%송세봉%리단%필룡%왕군%리국신%팽뢰
柠檬酸钙%骨形态发生蛋白质量%成骨细胞
檸檬痠鈣%骨形態髮生蛋白質量%成骨細胞
저몽산개%골형태발생단백질량%성골세포
Calcium citrate%Bone morphogenetic protein%Osteoblasts
目的 研究柠檬酸钙与骨形态发生蛋白-2(BMP-2)复合后成骨的作用.方法 取2月龄雄性昆明小鼠15只,通过体外实验获取柠檬酸钙与BMP-2最佳比例,在体外将10 mg柠檬酸钙与2mg BMP-2复合后,植入小鼠股部肌肉上中,行肌袋实验.以柠檬酸钙与BMP-2复合物为实验组,单独BMP-2为对照组,术后2、4、6周取材进行大体标本观察与组织学观察,计算新骨形成面积比及新生骨密度比.结果 大体标本观察与组织学观察示实验组成骨明显高于对照组,成骨面积较对照组明显增多.2、4、6周时比较差异均有统计学意义(P＜0.05)；2、4、6周时骨密度测量实验组较对照组高,差异均有统计学意义(P＜0.05).实验组骨质2周时出现骨小梁结构,成熟度高,成骨细胞数量多,分化明显,成骨密度高；4周时骨的成熟度增高,更多粉红色骨质形成,骨质基本成熟,骨小梁增粗增大,出现大面积成骨区域；6周时血管化及骨髓腔形成充分,骨质密度高.结论 柠檬酸钙与BMP-2复合后具有良好的诱导成骨作用,可促进骨形成,是一种较理想的载体材料,可作为一种新型复合人工骨修复骨缺损.
目的 研究檸檬痠鈣與骨形態髮生蛋白-2(BMP-2)複閤後成骨的作用.方法 取2月齡雄性昆明小鼠15隻,通過體外實驗穫取檸檬痠鈣與BMP-2最佳比例,在體外將10 mg檸檬痠鈣與2mg BMP-2複閤後,植入小鼠股部肌肉上中,行肌袋實驗.以檸檬痠鈣與BMP-2複閤物為實驗組,單獨BMP-2為對照組,術後2、4、6週取材進行大體標本觀察與組織學觀察,計算新骨形成麵積比及新生骨密度比.結果 大體標本觀察與組織學觀察示實驗組成骨明顯高于對照組,成骨麵積較對照組明顯增多.2、4、6週時比較差異均有統計學意義(P＜0.05)；2、4、6週時骨密度測量實驗組較對照組高,差異均有統計學意義(P＜0.05).實驗組骨質2週時齣現骨小樑結構,成熟度高,成骨細胞數量多,分化明顯,成骨密度高；4週時骨的成熟度增高,更多粉紅色骨質形成,骨質基本成熟,骨小樑增粗增大,齣現大麵積成骨區域；6週時血管化及骨髓腔形成充分,骨質密度高.結論 檸檬痠鈣與BMP-2複閤後具有良好的誘導成骨作用,可促進骨形成,是一種較理想的載體材料,可作為一種新型複閤人工骨脩複骨缺損.
목적 연구저몽산개여골형태발생단백-2(BMP-2)복합후성골적작용.방법 취2월령웅성곤명소서15지,통과체외실험획취저몽산개여BMP-2최가비례,재체외장10 mg저몽산개여2mg BMP-2복합후,식입소서고부기육상중,행기대실험.이저몽산개여BMP-2복합물위실험조,단독BMP-2위대조조,술후2、4、6주취재진행대체표본관찰여조직학관찰,계산신골형성면적비급신생골밀도비.결과 대체표본관찰여조직학관찰시실험조성골명현고우대조조,성골면적교대조조명현증다.2、4、6주시비교차이균유통계학의의(P＜0.05)；2、4、6주시골밀도측량실험조교대조조고,차이균유통계학의의(P＜0.05).실험조골질2주시출현골소량결구,성숙도고,성골세포수량다,분화명현,성골밀도고；4주시골적성숙도증고,경다분홍색골질형성,골질기본성숙,골소량증조증대,출현대면적성골구역；6주시혈관화급골수강형성충분,골질밀도고.결론 저몽산개여BMP-2복합후구유량호적유도성골작용,가촉진골형성,시일충교이상적재체재료,가작위일충신형복합인공골수복골결손.
Objective To study the osteogenetic effect of the compound of calcium citrate and recombinant human bone morphogenetic protein(rhBMP).Methods Fifteen male Kunming mice,2months of age,were used to obtain the optimal composition ratio of calcium citrate and rhBMP in an in vitro experiment.A compound of 10 mg of calcium citrate plus 2 mg of rhBMP was implanted into the mouse thigh muscle pouch in the experimental group while in the control group BMP alone was implanted.Samples were harvested at 2,4,6 weeks postoperation for gross and histological observations.The areas and densities of new bone formation in both groups were counted.Results At all time points,the new bone areas were significantly larger and the new bone densities were significantly greater in the experimental group than in the control group ( P ＜ 0.05).In the experimental group,more mature trabecular bone appeared at 2 weeks,with higher destiny and more proliferated and differentiated osteoblasts.At 4 weeks,there was a larger area of newly formed pink bone and larger bone trabeculae.At 6 weeks,vascularization appeared and medullary cavities formed with increased bone destiny.Conclusion Since the compound of calcium citrate and th-BMP has a fine effect on osteoinduction and osteogenesis,it can be used as a new compound artificial bone to repair bone defects.
