中华泌尿外科杂志
中華泌尿外科雜誌
중화비뇨외과잡지
CHINESE JOURNAL OF UROLOGY
2009年
6期
379-381
,共3页
丁冠雄%华立新%徐郑%冯宁翰%宋宁宏%张炜%吴宏飞
丁冠雄%華立新%徐鄭%馮寧翰%宋寧宏%張煒%吳宏飛
정관웅%화립신%서정%풍저한%송저굉%장위%오굉비
前列腺肿瘤%基因多态性%巨噬细胞移动抑制因子
前列腺腫瘤%基因多態性%巨噬細胞移動抑製因子
전렬선종류%기인다태성%거서세포이동억제인자
Prostatic neoplasms%Genetic polymorphism%Macrophage migration inhibitory factor
目的 探讨前列腺癌(PCa)患者外周血中巨噬细胞移动抑制因子(MIF)基因-173位点单核苷酸多态性在PCa发生中的作用.方法采用病例对照研究方法提取259例PCa、301例非肿瘤非前列腺疾病患者外周血中基因组DNA,应用PCR-限制性片段长度多态性分析MIF基因-173位点的多态性,比较不同基因型与PCa易感性的关系,并分析基因多态性与年龄、吸烟情况、肿瘤家族史的关系.结果 PCa患者中携带*C等位基因比例为36%,显著高于对照组的15%;携带MIF-173*C等位基因的个体PCa发病风险为G/G基因型的2.96倍(OR=2.96,95%CI:1.92～4.57);年龄>70岁、浅吸烟、有肿瘤家族史人群携带MIF-173*C等位基因的个体PCa发病风险显著高于G/G基因型个体,校正OR值分别为3.66(95%CI:2.02～6.62),2.83(95%CI:1.07～7.45)和3.26(95%CI:1.24～8.55).结论 MIF-173*C等位基因可能与PCa发生有关,年龄、吸烟情况、肿瘤家族史是PCa发病中的重要影响因素.
目的 探討前列腺癌(PCa)患者外週血中巨噬細胞移動抑製因子(MIF)基因-173位點單覈苷痠多態性在PCa髮生中的作用.方法採用病例對照研究方法提取259例PCa、301例非腫瘤非前列腺疾病患者外週血中基因組DNA,應用PCR-限製性片段長度多態性分析MIF基因-173位點的多態性,比較不同基因型與PCa易感性的關繫,併分析基因多態性與年齡、吸煙情況、腫瘤傢族史的關繫.結果 PCa患者中攜帶*C等位基因比例為36%,顯著高于對照組的15%;攜帶MIF-173*C等位基因的箇體PCa髮病風險為G/G基因型的2.96倍(OR=2.96,95%CI:1.92～4.57);年齡>70歲、淺吸煙、有腫瘤傢族史人群攜帶MIF-173*C等位基因的箇體PCa髮病風險顯著高于G/G基因型箇體,校正OR值分彆為3.66(95%CI:2.02～6.62),2.83(95%CI:1.07～7.45)和3.26(95%CI:1.24～8.55).結論 MIF-173*C等位基因可能與PCa髮生有關,年齡、吸煙情況、腫瘤傢族史是PCa髮病中的重要影響因素.
목적 탐토전렬선암(PCa)환자외주혈중거서세포이동억제인자(MIF)기인-173위점단핵감산다태성재PCa발생중적작용.방법채용병례대조연구방법제취259례PCa、301례비종류비전렬선질병환자외주혈중기인조DNA,응용PCR-한제성편단장도다태성분석MIF기인-173위점적다태성,비교불동기인형여PCa역감성적관계,병분석기인다태성여년령、흡연정황、종류가족사적관계.결과 PCa환자중휴대*C등위기인비례위36%,현저고우대조조적15%;휴대MIF-173*C등위기인적개체PCa발병풍험위G/G기인형적2.96배(OR=2.96,95%CI:1.92～4.57);년령>70세、천흡연、유종류가족사인군휴대MIF-173*C등위기인적개체PCa발병풍험현저고우G/G기인형개체,교정OR치분별위3.66(95%CI:2.02～6.62),2.83(95%CI:1.07～7.45)화3.26(95%CI:1.24～8.55).결론 MIF-173*C등위기인가능여PCa발생유관,년령、흡연정황、종류가족사시PCa발병중적중요영향인소.
Objective To study the association of MIF-173 locus polymorphism and the risk of prostate cancer (PCa) in China. Methods A case control study including 259 PCa patients and 301 age-matched controls was conducted. The polymorphisms of MIF-173 locus were analyzed by poly-merase chain reaction restriction fragment length polymorphism (PCR-RFLP) technique using genomic DNA isolated from peripheral blood lymphocytes. The correlations between the susceptibility to PCa and different genotypes were compared. The effect of age, smoking method and family history of canc-er were also analyzed. Results The rate of the MIF-173 * C variant allele of the PCa patients(n=259) was significantly higher than that of the controls (n=301) (36.0% vs 15.0%). The MIF-173 *C variant allele could significantly increase the risk of PCa (OR=2.96,95%CI: 1.92-4.57). Peo-ple with older age (age>70) or family history of cancer, who carried MIF-173 * C allele demonstra-ted a significantly increased risk in comparison with those carrying wild genotype of G/G(OR=3.66, 95%CI=2.02-6.62;OR=3.26, 95%CI=1.24-8.55). Conclusion These results suggested that polymorphisms of MIF-173 locus appear to influence the risk of PCa and may have synergistic effect with age and family history of cancer.
