CAJ | 학술논문

目的了解北京市性传播HIV-1感染者流行毒株亚型特点和流行规律.方法随机采集北京市2008年新确证性传播HIV感染者的抗凝全血标本100份,分离血浆,提取病毒RNA,用套式聚合酶链反应扩增病毒gag基因,并进行序列测定和亚型分析.结果系统进化分析确定北京市性传播HIV-1感染者流行毒株存在8个亚型或流行重组型,分别为B亚型22份,B'亚型8份,C亚型1份,CRF01_AE 38份,CRF02_AG 2份,CRF07 BC 9份,CRF08_BC 3份,疑似C/CRF01_AE重组型1份.结论 CRF01_AE和B亚型分别占45.2%和26.2%,为性传播感染者主要的亚型,应该加强我市HIV-1亚型流行情况的监测.
목적 료해북경시성전파HIV-1감염자류행독주아형특점화류행규률.방법 수궤채집북경시2008년신학증성전파HIV감염자적항응전혈표본100빈,분리혈장,제취병독RNA,용투식취합매련반응확증병독gag기인,병진행서렬측정화아형분석.결과 계통진화분석학정북경시성전파HIV-1감염자류행독주존재8개아형혹류행중조형,분별위B아형22빈,B'아형8빈,C아형1빈,CRF01_AE 38빈,CRF02_AG 2빈,CRF07 BC 9빈,CRF08_BC 3빈,의사C/CRF01_AE중조형1빈.결론 CRF01_AE화B아형분별점45.2%화26.2%,위성전파감염자주요적아형,응해가강아시HIV-1아형류행정황적감측.
Objective To investigate the subtype distribution and sequence characteristics of HIV-1 strains prevalent among sexual infectors in Beijing. Methods We collected the blood samples from 100HIV sexual infectors in Beijing during 2008 and separated plasma specimens. RNA was extracted from the plasma and the gag gene was amplified by RT-PCR and nest-PCR. The PCR products were sequenced directly and phylogenetic analyses of gag gene was performed using the MEGA4 software. Results Among 100 HIV-1 plasma samples,84 gag gene fragments were amplified and analyzed. Eight HIV subtypes including B(22 strains), B'(8 strains),C( 1 strain) ,CRF01_AE (38 strains) ,CRF02_AG (2 strains) ,CRF07_BC(9 strains) ,CRF08_BC(3 strains) and C/CRF01_AE recombinant like strain( 1 strain) were identified circulating in Beijing. Conclusion CRF01 _AE and subtype B were predominant in Beijing account for 45.2% and 26.2% and the surveillance of HIV gene variation should be paid more attention.