中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2009年
3期
171-173
,共3页
尤凤兴%居丽雯%马广源%张敬平%施强%吉杏生%吴家林%凌霞%肖勇%沙丹%吴庆刚%季亚勇
尤鳳興%居麗雯%馬廣源%張敬平%施彊%吉杏生%吳傢林%凌霞%肖勇%沙丹%吳慶剛%季亞勇
우봉흥%거려문%마엄원%장경평%시강%길행생%오가림%릉하%초용%사단%오경강%계아용
正粘病毒科%监测,免疫学%血凝素类%基因%变异(遗传学)
正粘病毒科%鑑測,免疫學%血凝素類%基因%變異(遺傳學)
정점병독과%감측,면역학%혈응소류%기인%변이(유전학)
Orthomyxovixidae%Monitoring,Immune%Hemagglutinins%Genes%Variation (Genetics)
目的 监测无锡地区2005年至2008年季节性流感型与亚型分布并了解2008年部分A/H3N2分离株血凝素(8A)基因变异状况.方法 无锡地区医院门诊流感样患者及集体单位聚集性流感样暴发患者采集鼻咽拭标本,接种MDCK细胞,采用标准抗血清鉴定阳性分离株型与亚型,并对2008年部分H3亚型流感病毒进行HA全基因测序,分析HA基因变异状况.结果 2005年至2008年9月,在无锡地区流感样患者中共分离到435株流感病毒,其中164株为A/H1N1亚型,80株为MH3N2亚型,B型191株.型与亚型有明显季节性分布强弱特征.H3亚型HA序列分析,无锡地区9株分离株与上海地区同期分离株接近,有很多序列相互穿插归属于进化树的同一分支,与WHO 2008-2009年疫苗推荐株相近.结论 近年无锡地区散发和局部暴发流感病毒感染仍主要为A/H1N1、A/H3N2和B型,A/H3N2 HA基因与上海地区同期分离株接近,与WHO 2008-2009年疫苗推荐株相近.
目的 鑑測無錫地區2005年至2008年季節性流感型與亞型分佈併瞭解2008年部分A/H3N2分離株血凝素(8A)基因變異狀況.方法 無錫地區醫院門診流感樣患者及集體單位聚集性流感樣暴髮患者採集鼻嚥拭標本,接種MDCK細胞,採用標準抗血清鑒定暘性分離株型與亞型,併對2008年部分H3亞型流感病毒進行HA全基因測序,分析HA基因變異狀況.結果 2005年至2008年9月,在無錫地區流感樣患者中共分離到435株流感病毒,其中164株為A/H1N1亞型,80株為MH3N2亞型,B型191株.型與亞型有明顯季節性分佈彊弱特徵.H3亞型HA序列分析,無錫地區9株分離株與上海地區同期分離株接近,有很多序列相互穿插歸屬于進化樹的同一分支,與WHO 2008-2009年疫苗推薦株相近.結論 近年無錫地區散髮和跼部暴髮流感病毒感染仍主要為A/H1N1、A/H3N2和B型,A/H3N2 HA基因與上海地區同期分離株接近,與WHO 2008-2009年疫苗推薦株相近.
목적 감측무석지구2005년지2008년계절성류감형여아형분포병료해2008년부분A/H3N2분리주혈응소(8A)기인변이상황.방법 무석지구의원문진류감양환자급집체단위취집성류감양폭발환자채집비인식표본,접충MDCK세포,채용표준항혈청감정양성분리주형여아형,병대2008년부분H3아형류감병독진행HA전기인측서,분석HA기인변이상황.결과 2005년지2008년9월,재무석지구류감양환자중공분리도435주류감병독,기중164주위A/H1N1아형,80주위MH3N2아형,B형191주.형여아형유명현계절성분포강약특정.H3아형HA서렬분석,무석지구9주분리주여상해지구동기분리주접근,유흔다서렬상호천삽귀속우진화수적동일분지,여WHO 2008-2009년역묘추천주상근.결론 근년무석지구산발화국부폭발류감병독감염잉주요위A/H1N1、A/H3N2화B형,A/H3N2 HA기인여상해지구동기분리주접근,여WHO 2008-2009년역묘추천주상근.
Objective To monitor the seasonal distribution of influenza types and subtypes in Wuxi area during 2005-2008, and to investigate the variation in hemagglutinin (HA) genes of MH3N2 strains in 2008. Methods Nose-throat swab specimens were collected in Wuxi area from flu-like patients from outpatient departments of hospitals as well as from clustering flu-like outbreak patients from workspace, followed by MDCK cell inoculation. Types and subtypes of positive influenza isolates were identified using standard antiserum. We then sequenced the HA genes for H3 subtype influenza viruses isolated from 2008 specimens to investigate the variation in HA genes. Results During 2005 and September 2008, 435 strains of influenza viruses were isolated from flu-like patients in Wuxi Area, among which 164 isolates are of A/H1N1 subtype, 80 isolates are of MH3N2 subtype, and 191 isolates are of B type. These types/subtypes have significant seasonal distributions. Sequences of HA genes for 113 subtype show that the 9 strains isolated in Wuxi area are similar to those of strains isolated in Shanghai within the same period. Many of the sequences belong to the same branch of the phylogenetic tree, and are similar to sequences of vaccine strains in WHO 2008-2009 repositories. Conclusion A/H1N1, MH3N2 and B still attribute to most of the sporadic and local outbreaks of influenza infection in Wuxi area in recent years. HAgenes of A/H3N2 strains isolated in Wuxi area are similar to those of strains isolated in Shanghai in the same period, and also similar to those of vaccine strains recommended by WHO for 2008-2009.
