上海医学检验杂志
上海醫學檢驗雜誌
상해의학검험잡지
SHANGHAI JOURNAL OF MEDICAL LABORATORY SCIENCES
2001年
1期
8-9,12
,共3页
多发性骨髓瘤%免疫固定电泳%免疫分型
多髮性骨髓瘤%免疫固定電泳%免疫分型
다발성골수류%면역고정전영%면역분형
目的 探讨多发性骨髓瘤(multiplemyeloma,MM)的血清免疫学特征。 方法 对28例MM患者的血清进行琼脂糖凝胶电泳及电泳扫描,血清蛋白和免疫球蛋白定量,以及免疫固定电泳。 结果 27例(96.4%)MM患者血清蛋白电泳可显示M带,IgG型、IgM型多在γ区,IgA型多在β区。不同型别MM免疫固定电泳图谱各异,IgG、IgA、IgM和单纯游离轻链的比例分别为46.4%、21.4%、17.9%、10.7%。在轻链型别中,IgG、IgM类M蛋白以κ型为主,单纯轻链型以λ型为主。免疫球蛋白定量显示M蛋白所属免疫球蛋白部分显著增高,非M蛋白所属免疫球蛋白显著降低。 结论 通过上述四种免疫化学综合实验对MM进行免疫分型,可对MM作出准确的实验室诊断。
目的 探討多髮性骨髓瘤(multiplemyeloma,MM)的血清免疫學特徵。 方法 對28例MM患者的血清進行瓊脂糖凝膠電泳及電泳掃描,血清蛋白和免疫毬蛋白定量,以及免疫固定電泳。 結果 27例(96.4%)MM患者血清蛋白電泳可顯示M帶,IgG型、IgM型多在γ區,IgA型多在β區。不同型彆MM免疫固定電泳圖譜各異,IgG、IgA、IgM和單純遊離輕鏈的比例分彆為46.4%、21.4%、17.9%、10.7%。在輕鏈型彆中,IgG、IgM類M蛋白以κ型為主,單純輕鏈型以λ型為主。免疫毬蛋白定量顯示M蛋白所屬免疫毬蛋白部分顯著增高,非M蛋白所屬免疫毬蛋白顯著降低。 結論 通過上述四種免疫化學綜閤實驗對MM進行免疫分型,可對MM作齣準確的實驗室診斷。
목적 탐토다발성골수류(multiplemyeloma,MM)적혈청면역학특정。 방법 대28례MM환자적혈청진행경지당응효전영급전영소묘,혈청단백화면역구단백정량,이급면역고정전영。 결과 27례(96.4%)MM환자혈청단백전영가현시M대,IgG형、IgM형다재γ구,IgA형다재β구。불동형별MM면역고정전영도보각이,IgG、IgA、IgM화단순유리경련적비례분별위46.4%、21.4%、17.9%、10.7%。재경련형별중,IgG、IgM류M단백이κ형위주,단순경련형이λ형위주。면역구단백정량현시M단백소속면역구단백부분현저증고,비M단백소속면역구단백현저강저。 결론 통과상술사충면역화학종합실험대MM진행면역분형,가대MM작출준학적실험실진단。
Objective To study the seroimmunological features of mutiplemyeloma(MM). Methods 28 serum samples of MM patients were analyzed by agarose gel electrophoresis and electrophoretic scanning, quantitative measurement of serum protein and immunoglubulins, and immunofixation electrophoresis. Results M bands appeared in 96.4% serum sample, in which IgG、 IgM appeared in γ zone and IgA appeared in β zone. The figure of immunofixation electrophoresis varied from types to types of MM, IgG, IgA, IgM and free light chain accounted for 46.4%, 21.4%, 17.9% and 10.7% respectively in all of the M protein. By classification of the light chain types, M proteins of IgG and IgM were mainly κ type, and the light chain types were λ type. Part of the concentration of immonoglobulin which contains monoclonal component increased significantly. Conclusions An overall consideration of the laboratory results from above methods is helpful in making a more accurate laboratory diagnosis for patients with multiple myeloma.