CAJ | 학술논문

医用植人体的成功与否常常取决于器件植入后细胞与材料表面间的相互作用.采用生物体外测试法考察了声电化学法制备的磷酸钙涂层对炭织物的骨细胞附着、增殖能力的影响.借助MTS检测技术、扫描电子显微镜,选择人类成骨细胞(MG63)作为细胞模型,通过测定细胞与炭织物、磷酸钙涂覆炭织物、以及其各自的提取液作用后的存活能力,研究了细胞/材料的相互作用,并对基底材料的细胞毒性进行了评价.结果表明,炭织物、磷酸钙涂覆炭织物均不具有细胞毒性,且磷酸钙涂层可提高成骨细胞的附着和增殖.SEM图像显示,细胞形貌正常,与对照组相比较生长增殖情况相似.
의용식인체적성공여부상상취결우기건식입후세포여재료표면간적상호작용.채용생물체외측시법고찰료성전화학법제비적린산개도층대탄직물적골세포부착、증식능력적영향.차조MTS검측기술、소묘전자현미경,선택인류성골세포(MG63)작위세포모형,통과측정세포여탄직물、린산개도복탄직물、이급기각자적제취액작용후적존활능력,연구료세포/재료적상호작용,병대기저재료적세포독성진행료평개.결과표명,탄직물、린산개도복탄직물균불구유세포독성,차린산개도층가제고성골세포적부착화증식.SEM도상현시,세포형모정상,여대조조상비교생장증식정황상사.
The success or failure of medical implants often depends on the cell-surface behavior after implantation of the device. This study investigated the use of woven carbon fabric, which had been sonoelectrochemically coated with calcium phosphate, to enhance bone cell attachment and proliferation in vitro. Human osteoblast-like cells, MG63, were used to study the interactions between cells and the material and assess the cytotoxicity of the substrates. The cytotoxicity of the materials was assessed using an MTS ((3-(4,5-dimethylthiazol-2 -yl ) -5 - ( 3-carboxymethoxyphenyl)-2-(4-sulfo- phenyl)-2H-tetrazolium, inner salt)) assay to determine the viability of the osteoblast-like MG63 cells in direct contact with the carbon fabric or calcium phosphate coated carbon fabrics, and to assess the cytotoxicity of extracts from these materials. The morphology of the surface adherent cells was assessed by scanning electron microscopy (SEM). Results showed that neither carbon fabrics nor calcium phosphate coated materials were cytotoxic. Furthermore, cell attachment and proliferation were enhanced by coating carbon fabrics with calcium phosphate. SEM showed that the cells had a nor- mal morphology and were well spread similar to those seen in the tissue culture plate control. These flexible calcium phosphate coated fabrics could, therefore, have uses in the reconstruction of bone in orthopaedic and dental surgery.