CAJ | 학술논문

以中国对虾抗WSSV选育群体第四代雌虾和野生中国对虾雄虾为亲本,采用人工精荚移植方式产生F1代家系,家系内个体姊妹交获得F2家系材料,42尾F2家系个体采用口饲法进行WSSV(White Spot Syndrome Virus)攻毒实验,获得个体抗WSSV及其它相关数据.构建了中国对虾的AFLP(Amplified Fragment Length Polymorphism)分子标记遗传连锁图谱.利用MAPMAKER/QTL1.1软件进行了中国对虾体长、全长、体重及抗WSSV性状的QTL(Quantitative Traits Loci)定位分析,首次实现了中国对虾重要经济性状的QTL定位.在LOD值大于2.0的条件下,共检测到和体长相关的QTL位点1个,与全长相关的QTL位点2个,与体重相关的QTL位点2个,与抗WSSV性状相关的位点2个,分别位于3个连锁群上,位点变异解释率从26.6%-66.9%不等.在其中的1个连锁群上检测到了体重、全长和抗WSSV性状相关的三个QTL位点,1个连锁群上检测到了体重和抗WSSV性状相关的两个QTL位点,1个连锁群上检测到了全长和体长相关的两个QTL位点.表明在中国对虾在此生长阶段,抗WSSV性状和个体大小存在一定程度的正相关关系[动物学报54(6):1075-1081,2008].
이중국대하항WSSV선육군체제사대자하화야생중국대하웅하위친본,채용인공정협이식방식산생F1대가계,가계내개체자매교획득F2가계재료,42미F2가계개체채용구사법진행WSSV(White Spot Syndrome Virus)공독실험,획득개체항WSSV급기타상관수거.구건료중국대하적AFLP(Amplified Fragment Length Polymorphism)분자표기유전련쇄도보.이용MAPMAKER/QTL1.1연건진행료중국대하체장、전장、체중급항WSSV성상적QTL(Quantitative Traits Loci)정위분석,수차실현료중국대하중요경제성상적QTL정위.재LOD치대우2.0적조건하,공검측도화체장상관적QTL위점1개,여전장상관적QTL위점2개,여체중상관적QTL위점2개,여항WSSV성상상관적위점2개,분별위우3개련쇄군상,위점변이해석솔종26.6%-66.9%불등.재기중적1개련쇄군상검측도료체중、전장화항WSSV성상상관적삼개QTL위점,1개련쇄군상검측도료체중화항WSSV성상상관적량개QTL위점,1개련쇄군상검측도료전장화체장상관적량개QTL위점.표명재중국대하재차생장계단,항WSSV성상화개체대소존재일정정도적정상관관계[동물학보54(6):1075-1081,2008].
A virgin female Chinese shrimp Fetmeropenaeis chomemsos was selected from the fourth generation of a successively WSSV (White Spot Syndrome Virus)resistance population and mated with a wild male through artificial insemination to produce the F1 and F2 generation.These shrimp were challenged with WSSV and the F2 generation were collected and scored for WSSV resistance,standard length,total body length and body weight.AFLP(Amplified Fragment Length Polymorphism) markers were used to construct a linkage map of F.chinensis based on this F2 family.The MAPMAKER/QTL1.1 software was applied in QTL (Quantitative Traits Loci)mapping of standard length,total body length,weight and WSSV-resistant of F.chinensis based on interval mapping method(IM).At the criterion of LOD(Limit of Detection)>2.0,one QTL associated with standard length,two with total body length.two with body weight and two with WSSV-resistance were detected.These QTL were distributed on three linkage groups.The variance-explained value QTLs were between 26.6%and 66.9%.Three QTL for body weight,total body length and WSSV-resistance were detected on LC11,two QTLs for standard length and total body length shared a same LG(Linkage Group),and two QTL of WSSV-resistance and body weight were distributed on another LG.These results suggested that the WSSV-resistance and the body shape traits of F.chinensis are correlated [Acta Zoologica Sinica 54(6):1075-1081,2008 ].