CAJ | 학술논문

以花脸蘑蛋白粗提液为对象,研究了其诱导烟草对TMV的抗性,对TMV的体外钝化作用及对TMV引起的番茄病毒病的室内控制作用;探讨了浓度、温度、pH值对花脸蘑蛋白粗提液抗TMV活性的影响.结果表明:当粗提液浓度为100 μg/mL时,诱导烟草抗TMV的侵染枯斑抑制率可达83.97%,对TMV的体外钝化作用的枯斑抑制率为74.57%,对番茄病毒病的室内控制效果达到82.70%,表明花脸蘑蛋白粗提液通过诱导抗病性和对TMV的体外钝化作用达到了控制TMV的作用;花脸蘑蛋白粗提液具有很好的温度稳定性,在50 ℃的条件下处理30 min,抗TMV侵染的枯斑抑制率仍达66.30%;花脸蘑蛋白粗提液也具有很好的pH值稳定性,pH敏感性试验表明,在pH为4.0～10.0的条件下,都具有抗TMV活性,在中性(pH=8)的条件下,活性最强可达81.50%.
이화검마단백조제액위대상,연구료기유도연초대TMV적항성,대TMV적체외둔화작용급대TMV인기적번가병독병적실내공제작용;탐토료농도、온도、pH치대화검마단백조제액항TMV활성적영향.결과표명:당조제액농도위100 μg/mL시,유도연초항TMV적침염고반억제솔가체83.97%,대TMV적체외둔화작용적고반억제솔위74.57%,대번가병독병적실내공제효과체도82.70%,표명화검마단백조제액통과유도항병성화대TMV적체외둔화작용체도료공제TMV적작용;화검마단백조제액구유흔호적온도은정성,재50 ℃적조건하처리30 min,항TMV침염적고반억제솔잉체66.30%;화검마단백조제액야구유흔호적pH치은정성,pH민감성시험표명,재pH위4.0～10.0적조건하,도구유항TMV활성,재중성(pH=8)적조건하,활성최강가체81.50%.
The crude protein extracted from Lepista sordida was studied,which can induce tobacco resistance to TMV, anti-TMV in vitro and control tomato virus disease caused by TMV. The effect on the anti-TMV activity of the protein by its concentration, temperature and pH were also studied. The results showed that when the concentration is 100μg/mL, the inhibition ratio of induced resistance to TMV and anti-TMV in vitro were 83.97% and 74.57%, respectively, the control ration of tomato virus disease was 82.70% in the greenhouse. When the crude protein was bathed at 50 ℃ for 30 min, the inhibition ratio of anti-TMV was 66.30%, and the results of pH test showed that crude protein from Lepista sordida can keep a high activity of anti-TMV between pH4.0-10.0, and its inhibition ratio of anti-TMV is 81.50% at pH8.0, which is the optimum pH.