CAJ | 학술논문

目的比较三种不同手术方法制作大鼠永久性脑缺血模型的效果,包括死亡率、神经功能评分、脑梗死体积、手术效率.方法将采用不同手术方法制备脑缺血模型的大鼠随机分为三组.1组在术中分别结扎颈总动脉(CCA)、颈外动脉(ECA)、枕动脉、翼腭动脉,并且用动脉夹对颈内动脉(ICA)进行临时夹闭;2组在术中分别结扎颈总动脉、颈外动脉,暴露枕动脉和翼腭动脉但不结扎,用丝线悬挂颈内动脉而不是用动脉夹夹闭,线栓在显微镜直视下插入颈内动脉越过翼腭动脉起始点至大脑中动脉分叉处;3组只暴露颈总动脉、颈外动脉和颈内动脉,结扎颈总动脉、颈外动脉,丝线悬挂颈内动脉,显微镜下将线栓盲插至颈内动脉大脑中动脉分叉处.分别检测三组模型的死亡率、神经功能评分、梗死体积、手术时间.结果第3组制作动物模型的方法所花费时间平均为17.5 min,死亡率较低,神经功能评分及梗死体积稳定.结论采用第3组手术方法可以缩短手术时间,提高手术效率,能够高效地制作出更加稳定的可用于临床实验的大鼠脑缺血模型.
목적 비교삼충불동수술방법 제작대서영구성뇌결혈모형적효과,포괄사망솔、신경공능평분、뇌경사체적、수술효솔.방법 장채용불동수술방법 제비뇌결혈모형적대서수궤분위삼조.1조재술중분별결찰경총동맥(CCA)、경외동맥(ECA)、침동맥、익악동맥,병차용동맥협대경내동맥(ICA)진행림시협폐;2조재술중분별결찰경총동맥、경외동맥,폭로침동맥화익악동맥단불결찰,용사선현괘경내동맥이불시용동맥협협폐,선전재현미경직시하삽입경내동맥월과익악동맥기시점지대뇌중동맥분차처;3조지폭로경총동맥、경외동맥화경내동맥,결찰경총동맥、경외동맥,사선현괘경내동맥,현미경하장선전맹삽지경내동맥대뇌중동맥분차처.분별검측삼조모형적사망솔、신경공능평분、경사체적、수술시간.결과 제3조제작동물모형적방법 소화비시간평균위17.5 min,사망솔교저,신경공능평분급경사체적은정.결론 채용제3조수술방법 가이축단수술시간,제고수술효솔,능구고효지제작출경가은정적가용우림상실험적대서뇌결혈모형.
Objective To compare and evaluate the efficacy of three operating procedures to produce permanent rat models of cerebral ischemia, including mortality, neurological evaluation score, infarction volume of ischemia and time consumed in the operation. Methods The rats were randomly divided into three groups. Group 1: The common carotid artery (CCA) and external carotid artery (ECA) were ligated during the operation and ICA was clipped temporarily by artery clamp. Group 2: The CCA and ECA were ligated and the superior thyroid artery and pterygopalatine artery were exposed but not ligated. Silk suture was utilized to hang the internal carotid artery (ICA). Plastic suture was inserted into ICA and stepped over the initiation point of pterygopalatine artery under microscopic observation. Group 3: The CCA and ECA were ligated and ICA was hanged with a silk suture, and the pterygopalatine artery was not exposed, but during the period of inserting plastic suture, the proximal part of the suture was pressed to make the suture's round distal end elevated, and then pass the initial point of pterygopalatine artery. Mortality, neurological score, volume of ischemic infarction and operation time consumed of the three groups were compared after the operation. Results The procedure to prepare the model was most efficient in the group 3, taking only 17.5 min to complete, significantly less than that in the group 1 (50 min) and group 2 (40 min), (P<0.05), and with a lower mortality and more steady neurological evaluation score and infarction volume. Conclusion The use of the third operating method can shorten the operation time and improve the efficacy of operation. Using this method, more consistent and repetitive focal cerebral ischemia models can be produced effectively, and meet the demands of clinical trials.