CAJ | 학술논문

鸡血浆样品用甲醇沉淀蛋白后,过DikmacC_(18)柱(100 mm×4.6 mm,5 μm),柱温:室温;流动相:甲醇-0.02mol/L乙酸胺缓冲液(28:72),pH4.9;流速:1.0 mL/min;检测波长:254 nm;检测器:紫外检测器;进样量:10μL.结果表明,检测线性范围为0.5～32.0 μg/mL(r=0.9999);最低检测浓度为0.25 μg/mL;回收率分别为92.5%、96.4%、98.9%;日内和日间变异系数均小于5%.该方法操作简便、灵敏、重现性好,适用于头孢拉定的临床药代动力学研究.
계혈장양품용갑순침정단백후,과DikmacC_(18)주(100 mm×4.6 mm,5 μm),주온:실온;류동상:갑순-0.02mol/L을산알완충액(28:72),pH4.9;류속:1.0 mL/min;검측파장:254 nm;검측기:자외검측기;진양량:10μL.결과표명,검측선성범위위0.5～32.0 μg/mL(r=0.9999);최저검측농도위0.25 μg/mL;회수솔분별위92.5%、96.4%、98.9%;일내화일간변이계수균소우5%.해방법조작간편、령민、중현성호,괄용우두포랍정적림상약대동역학연구.
After the plasma protein was precipitated, Cefradine was separated well on a Dikmac C_(18) clumn (100 mm×4.6 mm,5 μm)under room temperature. The mobile phase was a mixture of methanol-0.02mol/L ammonium acetate buffer solution (28:72 ,pH 4.9)at a rate of 1.0 mL/min. The UV detection was set at 254 nm. The results showed that the calibration curve was linear in the range of 0.5～32.0 μg/mL (r=0.9999). The lowest limit was 0.25 μg/mL and the recoveryies were 92.5%,96.4%, 98.9%. The RSD values of whithin-day and between-day precision were less than 5%. This method is simple, sensitive, accurate and reproducile. It is applicable for pharmacokinetic study of Cefraddine.