CAJ | 학술논문

测定了江苏太湖和广东汤溪水库7株铜绿微囊藻(M.aeruginosa)和1株水华微囊藻(M.flos-aquae)gyrB基因部分序尴旬(974bp),发现40个变异位点,17个简约信息位点,平均(G+C)%为47.8%,序列间相似性≥97.10%.在NJ分子系统树上,不同种类的微囊藻混杂分布,表明基因型聚类与表型无直接关系;不同地理来源的铜绿微囊藻间遗传变异小,没有明显的地理聚群,反映出地理差异并不影响遗传上的相似性;支持暂将不同藻种归为铜绿微囊藻复合种的分类处理.gyrB基因对微囊藻遗传差异的解析效果优于16S rRNA,与16S-23S ITS和cpcBA-IGS等效果相当,表明gyrB基因可能成为研究微囊藻分类和遗传变异新的良好分子标记.
측정료강소태호화엄동탕계수고7주동록미낭조(M.aeruginosa)화1주수화미낭조(M.flos-aquae)gyrB기인부분서감순(974bp),발현40개변이위점,17개간약신식위점,평균(G+C)%위47.8%,서렬간상사성≥97.10%.재NJ분자계통수상,불동충류적미낭조혼잡분포,표명기인형취류여표형무직접관계;불동지리래원적동록미낭조간유전변이소,몰유명현적지리취군,반영출지리차이병불영향유전상적상사성;지지잠장불동조충귀위동록미낭조복합충적분류처리.gyrB기인대미낭조유전차이적해석효과우우16S rRNA,여16S-23S ITS화cpcBA-IGS등효과상당,표명gyrB기인가능성위연구미낭조분류화유전변이신적량호분자표기.
In present study, partial sequences (974bp) of gyrB gene of 7 strains of M. aeruginosa and 1 strain of M. flos-aquae from Lake Taihu (Jiangsu Province ) and Tangxi Reservoir (Guangdong Province ) respectively were measured, and 40 variable points and 17 parsimony informative sites were found. The mean parameter of ( G +C)% was47.8%, and the sequence similarity among them were more than 97.10%. In the Neighbor-Joining Tree, different morphorspecies were intertwined and could not be discriminated clearly, indicating that genotypos clustering had no direcdy relations to phenotypes. The different geographical strains didn't clustered together either, indicating that geographic differences didn't affect genetic similarity, and supporting the taxonomic treatment that different species be classified as Microcystis aeruginosa complex-species temporarily. The resolution effect of gyrB gene to genetic diversity of Microcystis was better than 16S rRNA, and was similar to 16S -23S ITS and cpcBA-IGS, suggesting that gyrB gene may be a good molecular marker in studying the taxonomy and genetic variation of Microcystis.