CAJ | 학술논문

鱼类的耐低温性状是一种重要的经济性状.为了初步分析大黄鱼的耐低温性状,文章采用15对荧光微卫星标记,以SSR-PCR方法对大黄鱼低温耐受组和正常对照组F_1代共40个个体进行了耐低温性状遗传差异分析.结果显示,标记LYC0002在两组样品中共扩增出5个等位基因(片段大小分别为112 bp、110 bp、108 bp、106bp和104 bp),其中LYC0002_(112 bp)等位基因在低温耐受组的出现频率达60%,而在正常对照组中的频率为零,表明该等位基因对大黄鱼的温度敏感特性有较明显的偏好性,可能与某种耐低温基因存在一定的连锁关系.此外,对LYC0002_(106 bp)、LYC0002_(108 bp)、LYC0002_(110 bp)和LYC0002_(112 bp)4个等位基因分别进行了回收、克隆及测序.序列比对结果显示,LYC0002_(112 bp)等位基因含有10个(CA)重复单元,而其他3个等位基因依次缺失1个(CA)重复单元,说明LYC0002在本研究样本中的突变方式为微卫星逐步突变模型(Stepwise mutation model,SMM).
어류적내저온성상시일충중요적경제성상.위료초보분석대황어적내저온성상,문장채용15대형광미위성표기,이SSR-PCR방법대대황어저온내수조화정상대조조F_1대공40개개체진행료내저온성상유전차이분석.결과현시,표기LYC0002재량조양품중공확증출5개등위기인(편단대소분별위112 bp、110 bp、108 bp、106bp화104 bp),기중LYC0002_(112 bp)등위기인재저온내수조적출현빈솔체60%,이재정상대조조중적빈솔위령,표명해등위기인대대황어적온도민감특성유교명현적편호성,가능여모충내저온기인존재일정적련쇄관계.차외,대LYC0002_(106 bp)、LYC0002_(108 bp)、LYC0002_(110 bp)화LYC0002_(112 bp)4개등위기인분별진행료회수、극륭급측서.서렬비대결과현시,LYC0002_(112 bp)등위기인함유10개(CA)중복단원,이기타3개등위기인의차결실1개(CA)중복단원,설명LYC0002재본연구양본중적돌변방식위미위성축보돌변모형(Stepwise mutation model,SMM).
Cold tolerance is one of the major economic characters in fish.In order to discuss the cold tolerance of large yellow croaker (Pseudosciaena crocea R.),fifteen fluorescent dye-labeled microsatellite markers were applied to detect genetic differences between F_1 offsprings of cold tolerance group and normal group of large yellow croaker by SSR-PCR.Each group contained 20 randomly and separately sampled individuals.As a result,marker LYC0002 five alleles (LYC0002_(104bp),LYC0002_(106bp),LYC0002_(108bp),LYC0002_(110bp),and LYC0002_(112bp)) were amplified with marker LYC0002 in both groups and 60% (12/20) of individuals had allele LYC0002_(112bp) in cold tolerance group exclusively,which indicated that this allele is probably sensitive to temperature and associated with gene for cold tolerance.In addition,four alleles (LYC0002_(106bp),LYC0002_(108 bp),LYC0002_(110 bp),and LYC0002_(112bp)) were sequenced individually.Sequence alignments showed that LYC0002_(112 bp) allele contains 10 (CA) repeats,the remaining three alleles lacked one (CA) one by one,corresponding to the stepwise mutation model (SMM) of microsatellite.