四川省德格县2株疑似喜马拉雅旱獭鼠疫菌株的鉴定
사천성덕격현2주의사희마랍아한달서역균주적감정
Identification of 2 strains of suspected Yersinia pestis isolated from Marmot,,himalayana in Dege County,Sichuan Province
  • 万方数据
    中国地方病学杂志 중국지방병학잡지
    CHINESE JOURNAL OF ENDEMIOLOGY
    2009年 1期 48-53 ,共6页

    祁芝珍%罗志丹巴%段勇军%李敏%崔百忠%戴瑞霞%冯建萍%李存香%于守鸿%王祖郧%王虎%金星%赵海红%辛有全%任玲玲%张青雯%魏荣杰%金丽霞%金泳%熊浩明%罗孝林%泽仁桑珠%王洪%张珊瑚%郭文涛%王雪%丹巴泽里%翁丹%王代礼

    기지진%라지단파%단용군%리민%최백충%대서하%풍건평%리존향%우수홍%왕조운%왕호%금성%조해홍%신유전%임령령%장청문%위영걸%금려하%금영%웅호명%라효림%택인상주%왕홍%장산호%곽문도%왕설%단파택리%옹단%왕대례

    耶尔森菌,鼠疫%生物学鉴定%喜马拉雅旱獭%鼠疫自然疫源地 耶爾森菌,鼠疫%生物學鑒定%喜馬拉雅旱獺%鼠疫自然疫源地
    야이삼균,서역%생물학감정%희마랍아한달%서역자연역원지
    Yersinia pestis%Biologcal assay%Marmota hirnalayana%Plague natural focus
    目的 通过对四川省德格县自毙喜马拉雅旱獭体内分离出的2株疑似鼠疫菌株进行生化特征、毒力鉴定,确定四川省是否存在旱獭鼠疫自然疫源地.方法 菌株鉴定采用细菌形态染色、培养特性、鼠疫噬菌体裂解试验、生化及糖醇类酵解试验、毒力因子、毒力、营养需求、质粒、基因检测及基因分型方法进行.结果 光镜下,2株被检菌株均为革兰染色阴性,呈球杆状,两极浓染、两端钝圆;在赫氏普通琼脂上24-48 h,被检菌株形态呈中心发暗,有黄褐色粗糙颗粒,边缘不整呈锯齿状、薄而透明的花边;在赫氏血琼脂上,菌落隆起,色泽深,花边消失或残留锯齿状痕迹.2株被检菌株均能酵解阿胶糖、麦芽糖、甘油,不酵解鼠李糖、密二糖,脱氮阳性;毒力因子均为F1+、VW+、Pgm+,Pst I+;携带鼠疫菌常见的质粒,相对分子质量(Mr)为6.0×106、45.0×106、65.0×106,具有鼠疫菌的毒力相关质粒基因Pla、calfl、LcrG;对小鼠的毒力试验,绝对致死量(LD100)均为50个菌;营养需求上,对苯丙氨酸、甲硫氨酸依赖;基因型是Genomovar 5;与假结核菌(PTB)在鉴别培养基尿素、奥腾、枸橼酸盐上有明显区别,含鼠疫菌特有的Pst I和F1抗原,22℃下能被鼠疫噬菌体完全裂解.含有鼠疫菌特有的3a片段.结论 四川省德格县喜马拉雅早獭体内分离的2株疑似菌株均为鼠疫耶尔森菌,具有青藏高原喜马拉雅旱獭型鼠疫菌株的特性,四川省存在喜马拉雅旱獭鼠疫自然疫源地.
    목적 통과대사천성덕격현자폐희마랍아한달체내분리출적2주의사서역균주진행생화특정、독력감정,학정사천성시부존재한달서역자연역원지.방법 균주감정채용세균형태염색、배양특성、서역서균체렬해시험、생화급당순류효해시험、독력인자、독력、영양수구、질립、기인검측급기인분형방법진행.결과 광경하,2주피검균주균위혁란염색음성,정구간상,량겁농염、량단둔원;재혁씨보통경지상24-48 h,피검균주형태정중심발암,유황갈색조조과립,변연불정정거치상、박이투명적화변;재혁씨혈경지상,균락륭기,색택심,화변소실혹잔류거치상흔적.2주피검균주균능효해아효당、맥아당、감유,불효해서리당、밀이당,탈담양성;독력인자균위F1+、VW+、Pgm+,Pst I+;휴대서역균상견적질립,상대분자질량(Mr)위6.0×106、45.0×106、65.0×106,구유서역균적독력상관질립기인Pla、calfl、LcrG;대소서적독력시험,절대치사량(LD100)균위50개균;영양수구상,대분병안산、갑류안산의뢰;기인형시Genomovar 5;여가결핵균(PTB)재감별배양기뇨소、오등、구연산염상유명현구별,함서역균특유적Pst I화F1항원,22℃하능피서역서균체완전렬해.함유서역균특유적3a편단.결론 사천성덕격현희마랍아조달체내분리적2주의사균주균위서역야이삼균,구유청장고원희마랍아한달형서역균주적특성,사천성존재희마랍아한달서역자연역원지.
    Objective Throush identify biochemical characteristics and virulence factors of 2 strains suspected Yersinia pestis(Y.pestis)isolated from the dead Marmota himalayana(M.himalayana)to confirm the nature epidemic focus in Dege County,Sichuan Province.Methods Y.pestis was analyzed by specific staining and shape,culturing characteristics,splitting-test by bacteriophage,test of biochemical characteristics and glycolysis ability,virulence factors,virulence,nutritional requirement,plasmid,genetic test and genetic type. Results The tested strains were Gram staining bacilus.The main biochemical characteristics were Arabinose(+)、 Rhamnose(-),Maltose(+),Melibiose(-),Glycerol(+),Denitrification(+).The virulence factors with FI+.VW+, Pgm+,Pst I+;and with the common 6.0×106,45.0×106,65.0×106 plasmids,also with the virulence-relative plasmid gene.Both their absolutely lethal dose(LD100)in mice were 50 bacteria.The nutritional requirement appeared which were depended on Phenylalanine and Methionine.With the Genomovar 5 genotype characteristics of M.himalayana plague foci of Qinghai-Tibet plateau.The difference between tested strains and Yersinia pseudotubercuosis on the 3 different culture medium was obvious.The tested strains had a Y.pestis' specific 3a fragment,Pst I and FI-Ag,at 22 ℃,the strains could be split by bacteriophage completely.Conclusions According to the diagnostic criteria of plague in China,the 2 suspected strains isolated from Dege County,Sichuan Province ale confirmed as Y.pestis.both with powerful virulenceand with the characteristics of the Y.pestis of M.himahtyana in Qinghai-Tibet plateau plague natural focus.
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