CAJ | 학술논문

目的以狂犬病病毒为载体,构建表达丙型肝炎病毒包膜糖蛋白E1E2的狂犬/丙型肝炎嵌合病毒,为发展新型丙肝载体疫苗奠定基础.方法在狂犬病病毒反向遗传系统CTN-GFP的基础上,通过传统分子克隆方法,将HCV E1E2基因分别克隆人复制型和复制缺陷型狂犬病病毒载体,构建狂犬/丙肝嵌合病毒CTN-HCV E1E2和CTNΔG-HCV E1E2.结果免疫荧光(DFA)和反转录聚合酶链式反应(RT-PCR)结果显示嵌合病毒拯救成功,嵌合病毒能够再次感染正常细胞并且能够在mRNA水平检测到HCV E1E2基因的表达.结论本研究成功构建了表达丙型肝炎病毒包膜糖蛋白E1E2的狂犬/丙肝嵌合病毒,提示以狂犬病病毒为载体发展新型丙肝载体疫苗在理论和技术上都是可行的.
목적 이광견병병독위재체,구건표체병형간염병독포막당단백E1E2적광견/병형간염감합병독,위발전신형병간재체역묘전정기출.방법 재광견병병독반향유전계통CTN-GFP적기출상,통과전통분자극륭방법,장HCV E1E2기인분별극륭인복제형화복제결함형광견병병독재체,구건광견/병간감합병독CTN-HCV E1E2화CTNΔG-HCV E1E2.결과 면역형광(DFA)화반전록취합매련식반응(RT-PCR)결과 현시감합병독증구성공,감합병독능구재차감염정상세포병차능구재mRNA수평검측도HCV E1E2기인적표체.결론 본연구성공구건료표체병형간염병독포막당단백E1E2적광견/병간감합병독,제시이광견병병독위재체발전신형병간재체역묘재이론화기술상도시가행적.
Objective Construction and recovery of chimeric rabies virus expressing HCV envelop proteins E1E2. Methods On the basis of the previously established reverse genetic system CTN-CFP, HCV E1 E2 genes were cloned to both replication competent and replication constrained viral vectors based on CTN181 strain and the chimeric viruses CTN-HCV E1E2 and CTNAG-HCV E1E2 were recovered. Results The result demonstrated that both the chimeric viruses were rescued successfully, had the ability to reinfect normal sensitive cell lines and express HCV E1E2 genes detected in the level of mRNA. Conclusion The establishment of chimeric RVs expressing HCV E1E2 genes provides the evidence that it is feasible to develop novel HCV vaccines based on viral vectors in theory and in practice.