中华医学美学美容杂志
中華醫學美學美容雜誌
중화의학미학미용잡지
CHINESE JOURNAL OF MEDICAL AESTHETICS AND COSMETOLOGY
2012年
4期
282-285
,共4页
徐子寒%赵天兰%余道江%谢晓明%冀宇
徐子寒%趙天蘭%餘道江%謝曉明%冀宇
서자한%조천란%여도강%사효명%기우
窄蒂皮瓣%地拉罗司%缺氧诱导因子-1α%血管内皮细胞生长因子%微血管密度
窄蒂皮瓣%地拉囉司%缺氧誘導因子-1α%血管內皮細胞生長因子%微血管密度
착체피판%지랍라사%결양유도인자-1α%혈관내피세포생장인자%미혈관밀도
Narrow pedicle flap%Deferasirox%Hypoxia inducible factor-1a (HIF-1α)%Vascular endothelial growth factor (VEGF)%Microvessel density (MVD)
目的 探索术前应用地拉罗司(deferasirox)对大鼠乒乓拍样狭长窄蒂缺氧皮瓣模型微血管生成的促进作用及与用药时间的相关性.方法 成年雄性SD大鼠40只,随机分为对照组(8只),实验组(32只,分为1、3、5、7d组,每组8只).实验组:地拉罗司100 mg/kg分别连续灌胃1、3、5、7d;对照组:生理盐水100 mg/kg连续灌胃7d,灌胃结束后,次日行乒乓拍样窄蒂任意型皮瓣成形术,均于术后第7天取材,应用免疫组织化学、Western印迹及实时荧光定量聚合酶链式反应(PCR)法检测CD34表达,应用实时荧光定量PCR检测低氧诱导因子-1α(HIF-1α)和血管内皮生长因子(VEGF)的表达.结果 实验组较对照组皮瓣成活率明显提高,随着用药时间的增加,皮瓣微血管密度(MVD)、HIF-1α和VEGF均逐渐增高,7d达最高.结论 地拉罗司能诱导HIF-1α的分泌,促进VEGF产生,增加MVD表达,明显具有提高皮瓣对低氧的耐受,减少内皮细胞低氧后的坏死率,对缺血缺氧的窄蒂皮瓣具有较好的保护作用.
目的 探索術前應用地拉囉司(deferasirox)對大鼠乒乓拍樣狹長窄蒂缺氧皮瓣模型微血管生成的促進作用及與用藥時間的相關性.方法 成年雄性SD大鼠40隻,隨機分為對照組(8隻),實驗組(32隻,分為1、3、5、7d組,每組8隻).實驗組:地拉囉司100 mg/kg分彆連續灌胃1、3、5、7d;對照組:生理鹽水100 mg/kg連續灌胃7d,灌胃結束後,次日行乒乓拍樣窄蒂任意型皮瓣成形術,均于術後第7天取材,應用免疫組織化學、Western印跡及實時熒光定量聚閤酶鏈式反應(PCR)法檢測CD34錶達,應用實時熒光定量PCR檢測低氧誘導因子-1α(HIF-1α)和血管內皮生長因子(VEGF)的錶達.結果 實驗組較對照組皮瓣成活率明顯提高,隨著用藥時間的增加,皮瓣微血管密度(MVD)、HIF-1α和VEGF均逐漸增高,7d達最高.結論 地拉囉司能誘導HIF-1α的分泌,促進VEGF產生,增加MVD錶達,明顯具有提高皮瓣對低氧的耐受,減少內皮細胞低氧後的壞死率,對缺血缺氧的窄蒂皮瓣具有較好的保護作用.
목적 탐색술전응용지랍라사(deferasirox)대대서핑퐁박양협장착체결양피판모형미혈관생성적촉진작용급여용약시간적상관성.방법 성년웅성SD대서40지,수궤분위대조조(8지),실험조(32지,분위1、3、5、7d조,매조8지).실험조:지랍라사100 mg/kg분별련속관위1、3、5、7d;대조조:생리염수100 mg/kg련속관위7d,관위결속후,차일행핑퐁박양착체임의형피판성형술,균우술후제7천취재,응용면역조직화학、Western인적급실시형광정량취합매련식반응(PCR)법검측CD34표체,응용실시형광정량PCR검측저양유도인자-1α(HIF-1α)화혈관내피생장인자(VEGF)적표체.결과 실험조교대조조피판성활솔명현제고,수착용약시간적증가,피판미혈관밀도(MVD)、HIF-1α화VEGF균축점증고,7d체최고.결론 지랍라사능유도HIF-1α적분비,촉진VEGF산생,증가MVD표체,명현구유제고피판대저양적내수,감소내피세포저양후적배사솔,대결혈결양적착체피판구유교호적보호작용.
Objective To study the function of hypoxia mimics of different exposure time of deferasirox in improving the growth of micrangium in a narrow pedicle flap.Methods Fourty male SD rats were divided into 2 groups:experimental group was fed with deferasirox 100 mg/kg per day from 1d,3d,5d and 7d,respectively,before the surgery of transferring the narrow pedicle flap,while control group just fed with saline.After 7 days,the immunohistochemistry,Western blot and quantitative reverse transcription-PCR (qPCR) were performed to examine the expression of CD34.qPCR was used to examine the expression of HIF-1α,VEGF in order to investigate the regulatory effect of deferasirox in improving the growth of micrangium and the distinction among the different exposed time of deferasirox in the narrow pedicle flap.Results The deferasirox group exhibited a marked improvement in flap healing time,and with the increasing administration time of deferasirox,the expression of MVD,HIF-1α and VEGF was improved in each treated group (P＜0.05).Conclusions Deferasirox can induce HIF-1α secretion and increase CD34 expression,and so deferasirox can protect endothelial cells from hypoxic and ischaemic injury.
