中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2011年
21期
1457-1462
,共6页
王艳海%韩晓旭%安明晖%代娣%赵彬%沈书旭%楚振兴%徐俊杰%姜拥军%尚红
王豔海%韓曉旭%安明暉%代娣%趙彬%瀋書旭%楚振興%徐俊傑%薑擁軍%尚紅
왕염해%한효욱%안명휘%대제%조빈%침서욱%초진흥%서준걸%강옹군%상홍
HIV-1%感染%受体,CCR5%受体,CXCR4
HIV-1%感染%受體,CCR5%受體,CXCR4
HIV-1%감염%수체,CCR5%수체,CXCR4
HIV-1%Infection%Receptors,CCR5%Receptors,CXCR4
目的 观察HIV-1感染者在原发感染早期的不同阶段,体内病毒对CCR5和CXCR4辅助受体利用特点及演变情况.方法 以高危人群前瞻性队列研究的方式随访发现12例HIV-1原发感染者,分别收集感染最早期和病毒载量稳定期(病毒调定点)的血浆,提取病毒RNA,反转录(RT)-PCR,套式PCR扩增env gp160基因,连接到TA克隆载体,测序分析,以Geno2pheno、PSSMx4r5PSSMsinsi、SVM、Charge Rule 5种在线生物信息学工具预测病毒株CCB5和CXCR4辅助受体利用情况.结果 12例原发感染者感染早期病毒利用CCR5嗜性者有11例,利用CXCR4辅助受体1例;1例在随访10个月时检出辅助受体的转换;不同基因型预测方法的结果一致性88.3%.结论 HIV-1原发感染者早期病毒主要利用CCR5受体,也有利用CXCR4受体,原发感染者在感染1年内可能发生辅助受体的转换;以基因型预测CCB3或CXCR4辅助受体宜选用多种不同的分析方法.
目的 觀察HIV-1感染者在原髮感染早期的不同階段,體內病毒對CCR5和CXCR4輔助受體利用特點及縯變情況.方法 以高危人群前瞻性隊列研究的方式隨訪髮現12例HIV-1原髮感染者,分彆收集感染最早期和病毒載量穩定期(病毒調定點)的血漿,提取病毒RNA,反轉錄(RT)-PCR,套式PCR擴增env gp160基因,連接到TA剋隆載體,測序分析,以Geno2pheno、PSSMx4r5PSSMsinsi、SVM、Charge Rule 5種在線生物信息學工具預測病毒株CCB5和CXCR4輔助受體利用情況.結果 12例原髮感染者感染早期病毒利用CCR5嗜性者有11例,利用CXCR4輔助受體1例;1例在隨訪10箇月時檢齣輔助受體的轉換;不同基因型預測方法的結果一緻性88.3%.結論 HIV-1原髮感染者早期病毒主要利用CCR5受體,也有利用CXCR4受體,原髮感染者在感染1年內可能髮生輔助受體的轉換;以基因型預測CCB3或CXCR4輔助受體宜選用多種不同的分析方法.
목적 관찰HIV-1감염자재원발감염조기적불동계단,체내병독대CCR5화CXCR4보조수체이용특점급연변정황.방법 이고위인군전첨성대렬연구적방식수방발현12례HIV-1원발감염자,분별수집감염최조기화병독재량은정기(병독조정점)적혈장,제취병독RNA,반전록(RT)-PCR,투식PCR확증env gp160기인,련접도TA극륭재체,측서분석,이Geno2pheno、PSSMx4r5PSSMsinsi、SVM、Charge Rule 5충재선생물신식학공구예측병독주CCB5화CXCR4보조수체이용정황.결과 12례원발감염자감염조기병독이용CCR5기성자유11례,이용CXCR4보조수체1례;1례재수방10개월시검출보조수체적전환;불동기인형예측방법적결과일치성88.3%.결론 HIV-1원발감염자조기병독주요이용CCR5수체,야유이용CXCR4수체,원발감염자재감염1년내가능발생보조수체적전환;이기인형예측CCB3혹CXCR4보조수체의선용다충불동적분석방법.
Objective To evaluate the coreceptor usage characters and evolution of human immunodeficiency virus-1 ( HIV-1 ) primary infection. Methods A total of 12 HIV-1 primary infection cases were recruited from a high-risk population for this prospective cohort trial. The blood samples were collected at the earliest time after infection and after a viral setpoint respectively. RNA was extracted from plasma. The env genes were amplified through reverse transcription-PCR (RT-PCR) and nested PCR. And the env fragments were ligated to T vector and then sequenced. The pattern of coreceptor usage was predicted with five different online tools. Results Among them, 11 were predicted as CCR5 virus transmission and 1 as CXCR4 virus transmission. The viruses in 1 case were detected as a switch of coreceptor usage from CCR5 to CXCR4. The consistency of five different prediction rules was 88. 3%. Conclusion Although the CCR5 usage virus is predominant in primary infection through sexual transmission, CXCR4 usage virus can also be detected. A switch of coreceptor usage may happen within the first year post-infection. Adopting multiple rules may improve the efficiency and validity for the prediction of coreceptor usage based on genotypes.
