中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2010年
8期
704-709
,共6页
王红%魏欣净%贾青%沈永年%吕桂霞%刘维达
王紅%魏訢淨%賈青%瀋永年%呂桂霞%劉維達
왕홍%위흔정%가청%침영년%려계하%류유체
着色芽生菌病%动物试验%细胞因子%Th2/Th1
著色芽生菌病%動物試驗%細胞因子%Th2/Th1
착색아생균병%동물시험%세포인자%Th2/Th1
Chromoblastomycosis%Murine model%Cytokines%Th2/Th1
目的 利用小鼠皮下着色芽生菌病模型,研究在不同的病程发展阶段其T细胞免疫功能的变化.方法 足垫局部皮下注射法建立小鼠的裴氏着色霉感染的着色芽生菌病模型,通过免疫组织化学技术,检测正常小鼠足垫皮肤皮损局部细胞因子IFN-γ、IL-4、IL-10、TNF-α的表达并作为对照组,观察免疫正常组和环磷酰胺免疫抑制处理的免疫抑制组小鼠分别在感染上述真菌后7 d、30 d时,皮损局部细胞因子水平变化,并与对照组比较.结果 在着色芽生菌病小鼠模型中,第7天时,免疫正常组IL-4、TNF-α和IL-10较正常对照组均出现了显著的升高(P＜0.01),表现为Th1和Th2型细胞免疫均增强的模式,并以Th2型为主;30 d时IL-10表达显著下降(P＜0.01),与同期正常对照组比较差异无统计学意义(P=0.52),IFN-γ、TNF-α水平比7 d时显著升高(P＜0.01),表现为Th2型细胞免疫减弱Th1型占主导的模式.免疫功能受损组第7天时IL-10、IL-4的水平升高与其他两组相比差异均有统计学意义(P＜0.01),IFN-γ表达水平则明显下降(P＜0.01),TNF-α的表达与正常对照组相比差异无统计学意义(P=0.39),表现为Th2型细胞免疫增强Th1功能受抑模式;30 d时,IL-10表达水平较7 d时显著减少,但仍然高于同期的免疫正常组和正常对照组,IFN-γ、TNF-α水平显著升高(P＜0.01),但却低于免疫正常组,表现为Th2型免疫模式渐弱Th1功能逐渐增强模式.结论 在不同免疫状态下小鼠着色芽生菌病感染的过程中,随着病情好转存在由Th2型细胞免疫为主导转化为Th1型细胞免疫功能占主导的过程,免疫抑制下主要表现为Th1反应受抑制,Th1型细胞因子在控制着色芽生菌病的发展过程中具有关键性的保护作用,Th2型细胞因子则可能与感染的发展进程相关.
目的 利用小鼠皮下著色芽生菌病模型,研究在不同的病程髮展階段其T細胞免疫功能的變化.方法 足墊跼部皮下註射法建立小鼠的裴氏著色黴感染的著色芽生菌病模型,通過免疫組織化學技術,檢測正常小鼠足墊皮膚皮損跼部細胞因子IFN-γ、IL-4、IL-10、TNF-α的錶達併作為對照組,觀察免疫正常組和環燐酰胺免疫抑製處理的免疫抑製組小鼠分彆在感染上述真菌後7 d、30 d時,皮損跼部細胞因子水平變化,併與對照組比較.結果 在著色芽生菌病小鼠模型中,第7天時,免疫正常組IL-4、TNF-α和IL-10較正常對照組均齣現瞭顯著的升高(P＜0.01),錶現為Th1和Th2型細胞免疫均增彊的模式,併以Th2型為主;30 d時IL-10錶達顯著下降(P＜0.01),與同期正常對照組比較差異無統計學意義(P=0.52),IFN-γ、TNF-α水平比7 d時顯著升高(P＜0.01),錶現為Th2型細胞免疫減弱Th1型佔主導的模式.免疫功能受損組第7天時IL-10、IL-4的水平升高與其他兩組相比差異均有統計學意義(P＜0.01),IFN-γ錶達水平則明顯下降(P＜0.01),TNF-α的錶達與正常對照組相比差異無統計學意義(P=0.39),錶現為Th2型細胞免疫增彊Th1功能受抑模式;30 d時,IL-10錶達水平較7 d時顯著減少,但仍然高于同期的免疫正常組和正常對照組,IFN-γ、TNF-α水平顯著升高(P＜0.01),但卻低于免疫正常組,錶現為Th2型免疫模式漸弱Th1功能逐漸增彊模式.結論 在不同免疫狀態下小鼠著色芽生菌病感染的過程中,隨著病情好轉存在由Th2型細胞免疫為主導轉化為Th1型細胞免疫功能佔主導的過程,免疫抑製下主要錶現為Th1反應受抑製,Th1型細胞因子在控製著色芽生菌病的髮展過程中具有關鍵性的保護作用,Th2型細胞因子則可能與感染的髮展進程相關.
목적 이용소서피하착색아생균병모형,연구재불동적병정발전계단기T세포면역공능적변화.방법 족점국부피하주사법건립소서적배씨착색매감염적착색아생균병모형,통과면역조직화학기술,검측정상소서족점피부피손국부세포인자IFN-γ、IL-4、IL-10、TNF-α적표체병작위대조조,관찰면역정상조화배린선알면역억제처리적면역억제조소서분별재감염상술진균후7 d、30 d시,피손국부세포인자수평변화,병여대조조비교.결과 재착색아생균병소서모형중,제7천시,면역정상조IL-4、TNF-α화IL-10교정상대조조균출현료현저적승고(P＜0.01),표현위Th1화Th2형세포면역균증강적모식,병이Th2형위주;30 d시IL-10표체현저하강(P＜0.01),여동기정상대조조비교차이무통계학의의(P=0.52),IFN-γ、TNF-α수평비7 d시현저승고(P＜0.01),표현위Th2형세포면역감약Th1형점주도적모식.면역공능수손조제7천시IL-10、IL-4적수평승고여기타량조상비차이균유통계학의의(P＜0.01),IFN-γ표체수평칙명현하강(P＜0.01),TNF-α적표체여정상대조조상비차이무통계학의의(P=0.39),표현위Th2형세포면역증강Th1공능수억모식;30 d시,IL-10표체수평교7 d시현저감소,단잉연고우동기적면역정상조화정상대조조,IFN-γ、TNF-α수평현저승고(P＜0.01),단각저우면역정상조,표현위Th2형면역모식점약Th1공능축점증강모식.결론 재불동면역상태하소서착색아생균병감염적과정중,수착병정호전존재유Th2형세포면역위주도전화위Th1형세포면역공능점주도적과정,면역억제하주요표현위Th1반응수억제,Th1형세포인자재공제착색아생균병적발전과정중구유관건성적보호작용,Th2형세포인자칙가능여감염적발전진정상관.
Objective To evaluate the cell-mediated immune tissue reactions of mice model of chromoblastomycosis. Methods First we developed a murine model of chromoblastomycosis subcutaneous infection with F. pedrosoi inoculated into the footpads using immunocompetent and immunocompromised mice immuned by CTX, and then by immunohistochemistry methods analyzed the expression of cytokines IL-4, IL-10, TNF-α and IFN-γ at the seventh and thirtieth day, the expression of these cytokines at the same time in the healthy mice footpads were used as control. Results In the immunocompetent mice, The expression of IL-4, IL-10 and TNF-α was significantly higher when compared with healthy control at the seventh day,showing an up-regulation pattern of Th2 and Th1 type cellular immune functions with a predominance of the Th2 response. At the 30th day, the expression of IL-10 was significantly lower when compared with the 7th day and no difference with healthy controls, while IFN-γand TNF-α were gradually increased, the T cellmediated immune drives to Th1 response from Th2. In the immunocompromised mice, the expression of IL4 and IL-10 were significantly higher, meanwhile IFN-γwas lower than those in immunocompetent or healty mice, the levels of TNF-α was not significantly different fiom healty control at the 7th day, it showed that Th2 response was more increased with the Th1 responses was significantly inhibited in the early immune reactions. At the 30th day, the Th1 type cytokines IFN-γ and TNF-α were highly significantly higher than early stage but still lower than immunocompetent levels, the level of Th2 type cytokine IL-10 rradually decreased although it was still above the other two rroups. Conclusion In different immune state, there was an immune defence translation from the predominance of Th2 type cellular immunity to Th1 type in the process of murine model of chromoblastomycosis. Thl type cytokines which favors resistance to fungal disease, played a major role at controlling the development of chromoblastomycosis.
