CAJ | 학술논문

背景:目前国外的研究认为骨改建的过程可能部分地受神经肽调控.目的:观察低强度激光照射对大鼠实验性牙齿移动牙髓与牙周组织中P物质表达的影响.设计:随机对照的研究.地点和材料:于吉林大学第二临床学院动物室对45只Wistar大鼠进行动物实验.干预:利用免疫组化ABC染色,结合计算机图像分析,测定45只Wistar大鼠正畸牙齿移动在0,,6,2,4和72 h的实验侧和对照侧牙髓P物质免疫组化(SP-like immumoreactivity,P-IR);以及在0(0 h),(24 h),(72 h),,14和21 d时实验侧和对照侧牙周组织中SP-IR的灰度值.实验动物被分为9组,每组5只.于上颌第一磨牙和切牙之间拴结镍钛闭合螺旋弹簧,力值为40 g.左侧为实验侧,右侧为对照侧.实验侧为低强度激光照射侧,采用He-Ne激光与C02激光同时照射的方法.主要观察指标:①牙髓中SP-IR灰度值变化.②牙周膜压力区SP-IR灰度值变化.③牙周膜张力区SP-IR灰度值变化.④实验侧和对照侧的牙周膜压力区与张力区SP-IR灰度值变化的比较.结果:实验侧牙髓中SP-IR免疫组化灰度值低于对照侧,以第12,4 h明显(P＜0.01);实验侧牙周膜压力区SP-IR免疫组化灰度值在7 d时明显高于对照侧(P＜0.01);在14 d时较高于对照侧(P＜0.05).实验侧牙周膜张力区SP-IR免疫组化灰度值在14 d时较高于对照侧(P＜0.05).结论:低强度激光照射对正畸初始牙髓中P物质的表达及牙周组织中P物质的表达均有影响.
배경:목전국외적연구인위골개건적과정가능부분지수신경태조공.목적:관찰저강도격광조사대대서실험성아치이동아수여아주조직중P물질표체적영향.설계:수궤대조적연구.지점화재료:우길림대학제이림상학원동물실대45지Wistar대서진행동물실험.간예:이용면역조화ABC염색,결합계산궤도상분석,측정45지Wistar대서정기아치이동재0,,6,2,4화72 h적실험측화대조측아수P물질면역조화(SP-like immumoreactivity,P-IR);이급재0(0 h),(24 h),(72 h),,14화21 d시실험측화대조측아주조직중SP-IR적회도치.실험동물피분위9조,매조5지.우상합제일마아화절아지간전결얼태폐합라선탄황,력치위40 g.좌측위실험측,우측위대조측.실험측위저강도격광조사측,채용He-Ne격광여C02격광동시조사적방법.주요관찰지표:①아수중SP-IR회도치변화.②아주막압력구SP-IR회도치변화.③아주막장력구SP-IR회도치변화.④실험측화대조측적아주막압력구여장력구SP-IR회도치변화적비교.결과:실험측아수중SP-IR면역조화회도치저우대조측,이제12,4 h명현(P＜0.01);실험측아주막압력구SP-IR면역조화회도치재7 d시명현고우대조측(P＜0.01);재14 d시교고우대조측(P＜0.05).실험측아주막장력구SP-IR면역조화회도치재14 d시교고우대조측(P＜0.05).결론:저강도격광조사대정기초시아수중P물질적표체급아주조직중P물질적표체균유영향.
BACKGROUND: As believed by recent domestic and international researches,the bone reconstruction process might be partly modulated by neuropeptides.OBJECTIVE: To investigate the effect of low power laser irradiation on the expression of substance P (SP) in dental pulp and periodontium during experimental orthodontic tooth movement in rats.DESIGN: A randomized controlled trial SETTING and MATERIALS: Forty-five Wistar rats were selected in the study in the animal laboratory of the Second Clinic Medicine College of Jilin University.INTERVENTIONS: The immunohistochemical reactivity of SP(SP-IR) in dental pulp on both study side and control side of 45 Wistar rats with orthodontic tooth movement was assayed at 0,3,6,12,24,and 72 hours,as well as the gray scale of SP-IR in periodontium on both study side and control side at 0 (0 hour),1(24 hours),3(72 hours),7,14,and 21 days by immunohistochemical ABC staining combined with computerized image analysis system. Forty-five Wistar rats were allocated into 9 groups according to experimental time of 0,3,6,12,24 hours,3 days,7 days,14 days,and 21 days with 5 rats each. A NiTi coil spring with 40g connected the maxillary upper first molar to the upper incisor and fixed bilaterally by a ligature wire. The left side was set as study side and the right side was set as control side. Study side received low energy laser irradiation irradiated by He-Ne laser and CO2laser simultaneously.gray scale alterations at periodontal pressure and tension side between study side and control side.RESULTS: The dental pulp SP-IR gray scale was lower in study side than that of control side,with most significant alteration at 12 hours,and 24hours ( P ＜ 0. 01 ). Periodontal pressure side SP-IR gray scale in study side was significantly higher than that of control side at the 7th day ( P ＜ 0.01 )and higher than control side at the 14th day ( P ＜ 0.05). At tension sides of study sides,the periodontal SP-IR gray scale was higher than that of control sides at the 14th day ( P ＜ 0.05).CONCLUSION: Low energy laser affects SP expressions in both dental pulp and periodontium during the initial orthodontic tooth movement.